MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2102 493.2455 492.2382
2103 493.2479 492.2406
2104 494.2039 493.1966
2105 495.0799 494.0726
2106 495.2578 494.2505
2107 495.2585 494.2512
2108 495.2590 494.2517
2109 495.2594 494.2521
2110 495.2603 494.2530
2111 495.2684 494.2611
2112 496.2876 495.2803
2113 497.1704 496.1631
2114 497.2191 496.2118
2115 497.2366 496.2293
2116 497.2738 496.2665
2117 497.2750 496.2677
2118 499.1068 498.0995
2120 502.2892 501.2819
2121 502.3352 501.3279
2123 503.2472 502.2399
2124 503.3084 502.3011
2126 507.0790 506.0717
2127 507.2198 506.2125
2128 508.2778 507.2705
2129 508.2878 507.2805
2130 508.2887 507.2814
2131 508.2887 507.2814
2132 509.0923 508.0850
2133 509.1978 508.1905
2134 509.3449 508.3376
2135 510.2361 509.2288
2136 511.2549 510.2476
2137 512.3199 511.3126
2138 513.1426 512.1353
2140 515.0997 514.0924
2142 516.2576 515.2503
2147 517.2635 516.2562
2149 520.2302 519.2229
2150 520.3340 519.3267
2151 523.0746 522.0673
2152 524.3183 523.3110
2153 525.3223 524.3150
2157 529.0911 528.0838
2161 530.2953 529.2880
2163 531.1753 530.1680
2164 531.2224 530.2151
2167 535.0914 534.0841
2168 535.2508 534.2435
2169 537.0892 536.0819
2170 537.1159 536.1086
2171 537.2321 536.2248
2172 538.0376 537.0303
2173 538.1516 537.1443
2174 538.2970 537.2897
2175 538.2993 537.2920
2181 541.2946 540.2873
2182 543.2689 542.2616
2189 547.1705 546.1632
2192 547.3329 546.3256
2194 555.0272 554.0199
2195 555.1053 554.0980
2198 558.2678 557.2605
2199 559.2859 558.2786
2201 560.0685 559.0612
2205 563.1416 562.1343
2206 563.2845 562.2772
2207 564.3599 563.3526
2208 567.2417 566.2344
2209 567.2418 566.2345
2210 568.3467 567.3394
2211 569.3153 568.3080
2212 569.3662 568.3589
2213 571.3583 570.3510
2217 573.1129 572.1056
2224 581.2581 580.2508
2225 583.2721 582.2648
2228 588.0989 587.0916
2230 589.1838 588.1765
2239 594.3979 593.3906
2240 595.2726 594.2653
2241 595.2731 594.2658
2242 596.3519 595.3446
2243 596.3773 595.3700
2244 598.2258 597.2185
2246 300.5279 599.0412
2248 300.6666 599.3186
2249 301.1292 600.2438
2253 302.0840 602.1534
2255 604.0934 603.0861
2257 302.6645 603.3144
2261 609.3369 608.3296
2263 307.1019 612.1892
2264 307.1659 612.3172
2265 307.5927 613.1708
2266 307.6330 613.2514
2267 614.2591 613.2518
2268 307.6674 613.3202
2269 307.6982 613.3818
2275 309.0158 616.0170
2276 309.0417 616.0688
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