MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1993 457.0576 456.0503
1994 457.1392 456.1319
1996 458.3099 457.3026
1997 458.9089 457.9016
1998 459.2088 458.2015
1999 459.2211 458.2138
2000 459.2813 458.2740
2001 461.2506 460.2433
2002 462.0872 461.0799
2003 462.2315 461.2242
2004 462.2319 461.2246
2005 463.2327 462.2254
2006 463.2329 462.2256
2007 463.2336 462.2263
2008 463.2344 462.2271
2009 463.2368 462.2295
2010 465.2104 464.2031
2011 465.2106 464.2033
2012 465.2107 464.2034
2013 465.2107 464.2034
2014 465.2116 464.2043
2015 465.2118 464.2045
2016 465.2119 464.2046
2017 465.2501 464.2428
2018 465.2525 464.2452
2019 466.0434 465.0361
2020 467.1651 466.1578
2021 467.2255 466.2182
2022 467.2262 466.2189
2023 467.2264 466.2191
2024 467.2266 466.2193
2025 467.2268 466.2195
2026 467.2271 466.2198
2027 467.2274 466.2201
2028 467.2278 466.2205
2029 467.2719 466.2646
2030 468.0727 467.0654
2031 468.2686 467.2613
2032 468.2924 467.2851
2033 469.0527 468.0454
2034 469.2062 468.1989
2035 469.2420 468.2347
2036 469.2426 468.2353
2037 469.2433 468.2360
2038 470.2986 469.2913
2039 470.4219 469.4146
2040 471.0735 470.0662
2041 471.0749 470.0676
2042 471.1555 470.1482
2043 471.2462 470.2389
2044 472.2161 471.2088
2045 472.2531 471.2458
2046 472.2881 471.2808
2047 473.1530 472.1457
2048 473.1611 472.1538
2049 473.2505 472.2432
2050 473.2729 472.2656
2053 475.3264 474.3191
2054 476.2832 475.2759
2055 476.2834 475.2761
2056 476.2836 475.2763
2057 476.3081 475.3008
2058 477.2118 476.2045
2059 477.2133 476.2060
2060 477.2520 476.2447
2061 479.2269 478.2196
2062 479.2269 478.2196
2063 479.2272 478.2199
2064 479.2274 478.2201
2065 479.2281 478.2208
2066 479.2293 478.2220
2067 479.2646 478.2573
2068 479.2652 478.2579
2069 479.2652 478.2579
2070 479.2689 478.2616
2071 481.0639 480.0566
2072 481.2064 480.1991
2073 481.2064 480.1991
2074 481.2086 480.2013
2075 481.2293 480.2220
2076 481.2435 480.2362
2077 481.2435 480.2362
2078 481.2635 480.2562
2079 481.3135 480.3062
2080 483.1818 482.1745
2081 484.0692 483.0619
2082 484.2769 483.2696
2083 484.2889 483.2816
2084 485.2157 484.2084
2085 485.2950 484.2877
2086 487.1279 486.1206
2089 489.0820 488.0747
2094 491.2689 490.2616
2095 493.0178 492.0105
2096 493.0205 492.0132
2097 493.1962 492.1889
2098 493.2428 492.2355
2099 493.2430 492.2357
2100 493.2433 492.2360
2101 493.2438 492.2365
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