MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2683a |
| MS data file | : | Andy2683a.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 2 Aug 2026 at 00:54:18 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,770 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 2469 |
357.1380 | 712.2614 | 712.2850 | -33.1 | 0 | 10 | 0.094 | 1Score > 13 indicates identity |
MQYDR + Deamidated (NQ) |
| 3219 |
433.5802 | 1297.7188 | 1297.6667 | 40.2 | 0 | 10 | 0.66 | 1Score > 21 indicates identity |
TFDTNITAIFR |
| 2179 |
539.3553 | 538.3480 | 538.3227 | 47.0 | 1 | 10 | 0.094 | 1Score > 13 indicates identity |
IKAAH |
| 2630 |
434.2748 | 866.5350 | 866.5086 | 30.5 | 0 | 10 | 0.17 | 1Score > 15 indicates identity |
KPRPVNR + Deamidated (NQ) |
| 2923 |
1078.4128 | 1077.4055 | 1077.4574 | -48.2 | 0 | 10 | 0.094 | 1Score > 13 indicates identity |
TADATQEANR + 2 Deamidated (NQ) |
| 2369 |
650.3281 | 649.3208 | 649.3006 | 31.2 | 0 | 10 | 0.37 | 1Score > 21 indicates identity Score > 18 indicates homology |
ASWMR |
| 2119 |
499.2876 | 498.2803 | 498.2914 | -22.3 | 0 | 10 | 0.24 | 1Score > 17 indicates identity |
LGPGR |
| 2324 |
634.3320 | 633.3247 | 633.3082 | 26.1 | 0 | 10 | 0.73 | 1Score > 21 indicates identity |
SNSGLR + Deamidated (NQ) |
| 3827 |
728.3721 | 2182.0945 | 2182.0212 | 33.6 | 1 | 10 | 0.91 | 1Score > 23 indicates identity Score > 22 indicates homology |
AYMSIIIDDLGQSPERDSR + Oxidation (M); Deamidated (NQ) |
| 2327 |
634.3342 | 633.3269 | 633.3082 | 29.6 | 1 | 10 | 0.18 | 1Score > 21 indicates identity Score > 15 indicates homology |
ESKDR |
| 2910 |
533.2731 | 1064.5316 | 1064.4855 | 43.3 | 1 | 10 | 0.53 | 1Score > 22 indicates identity Score > 20 indicates homology |
RIEMCANR + Oxidation (M) |
| 2804 |
1007.4819 | 1006.4746 | 1006.5084 | -33.5 | 0 | 10 | 0.64 | 1Score > 22 indicates identity Score > 21 indicates homology |
QLVFTDAGR + Deamidated (NQ) |
| 3878 |
1116.5690 | 2231.1234 | 2231.0892 | 15.4 | 1 | 10 | 0.79 | 1Score > 23 indicates identity Score > 22 indicates homology |
ILDNAFLMSSSRNYIDSLR + Oxidation (M); Deamidated (NQ) |
| 2785 |
498.3047 | 994.5948 | 994.5923 | 2.52 | 0 | 10 | 0.18 | 1Score > 15 indicates identity |
LQPLLAGQR |
| 3232 |
656.3876 | 1310.7606 | 1310.7030 | 44.0 | 1 | 10 | 0.42 | 1Score > 19 indicates identity |
VGSIMHRLGWR |
| 3837 |
734.3759 | 2200.1059 | 2200.1483 | -19.3 | 1 | 10 | 0.22 | 1Score > 23 indicates identity Score > 16 indicates homology |
DKTLEMPVLILPSVQVNMR + Oxidation (M); 2 Deamidated (NQ) |
| 2639 |
441.2447 | 880.4748 | 880.4477 | 30.9 | 0 | 10 | 0.42 | 1Score > 19 indicates identity |
FMALVER + Oxidation (M) |
| 3542 |
854.3736 | 1706.7326 | 1706.8145 | -47.9 | 1 | 10 | 0.4 | 1Score > 19 indicates identity |
SCLQQRGEVQTALSK + 3 Deamidated (NQ) |
| 3522 |
843.4602 | 1684.9058 | 1684.9294 | -14.0 | 1 | 10 | 0.79 | 1Score > 22 indicates identity |
APLSNLMGKAQVALTR + Oxidation (M) |
| 2389 |
659.4357 | 658.4284 | 658.4377 | -14.1 | 1 | 10 | 0.19 | 1Score > 15 indicates identity |
LISKAK |
| 4209 |
498.0505 | 2485.2161 | 2485.1972 | 7.62 | 1 | 10 | 1 | 1Score > 23 indicates identity Score > 22 indicates homology |
NEETLEAHTYLEKLAPDINQR + 2 Deamidated (NQ) |
| 3427 |
504.5965 | 1510.7677 | 1510.7238 | 29.0 | 1 | 10 | 0.3 | 1Score > 22 indicates identity Score > 17 indicates homology |
EPMELFVDFRGR + Oxidation (M) |
| 2204 |
560.2736 | 559.2663 | 559.2714 | -9.12 | 0 | 10 | 0.29 | 1Score > 20 indicates identity Score > 17 indicates homology |
VGNDR |
| 3319 |
469.8958 | 1406.6656 | 1406.7041 | -27.4 | 1 | 10 | 0.62 | 1Score > 22 indicates identity Score > 20 indicates homology |
QFNDATLAELKR + 2 Deamidated (NQ) |
| 3357 |
720.3142 | 1438.6138 | 1438.6432 | -20.4 | 0 | 10 | 0.39 | 1Score > 18 indicates identity |
QMSPMSAEATVVR + 2 Oxidation (M); Deamidated (NQ) |
| 3643 |
909.9744 | 1817.9342 | 1817.8997 | 19.0 | 0 | 10 | 1 | 1Score > 23 indicates identity Score > 22 indicates homology |
LLALFPAVMCYWYR + Oxidation (M) |
| 3549 |
856.4269 | 1710.8392 | 1710.7631 | 44.5 | 1 | 10 | 0.62 | 1Score > 22 indicates identity Score > 20 indicates homology |
ESQVFADSIRDCER |
| 2977 |
553.3215 | 1104.6284 | 1104.5928 | 32.3 | 0 | 10 | 0.58 | 1Score > 20 indicates identity |
AIFVLDTNGR |
| 2515 |
383.7147 | 765.4148 | 765.4358 | -27.4 | 1 | 10 | 0.39 | 1Score > 18 indicates identity |
GIRGHAR |
| 3060 |
594.8034 | 1187.5922 | 1187.6510 | -49.4 | 1 | 10 | 0.61 | 1Score > 23 indicates identity Score > 20 indicates homology |
TQKDNLLSLR + Deamidated (NQ) |
| 3335 |
475.2224 | 1422.6454 | 1422.6483 | -2.05 | 0 | 10 | 0.19 | 1Score > 20 indicates identity Score > 15 indicates homology |
QMSPMSAEATVVR + Oxidation (M); Deamidated (NQ) |
| 3071 |
401.2239 | 1200.6499 | 1200.5921 | 48.1 | 1 | 10 | 0.64 | 1Score > 23 indicates identity Score > 20 indicates homology |
RADMLLDNPR + Deamidated (NQ) |
| 3198 |
641.7890 | 1281.5634 | 1281.6248 | -47.9 | 1 | 10 | 0.51 | 1Score > 19 indicates identity |
DRHCLESLPR |
| 2429 |
344.2059 | 686.3972 | 686.4188 | -31.3 | 1 | 10 | 0.66 | 1Score > 23 indicates identity Score > 20 indicates homology |
GRVVTR |
| 2477 |
720.3489 | 719.3416 | 719.3385 | 4.41 | 0 | 9 | 0.88 | 1Score > 21 indicates identity |
SICQGR |
| 3235 |
658.8702 | 1315.7258 | 1315.6731 | 40.1 | 1 | 9 | 0.28 | 1Score > 22 indicates identity Score > 16 indicates homology |
QAKASENLGELR + Deamidated (NQ) |
| 3408 |
747.8379 | 1493.6612 | 1493.6932 | -21.4 | 1 | 9 | 0.17 | 1Score > 20 indicates identity Score > 14 indicates homology |
ADGEAFMARQQLR + 2 Deamidated (NQ) |
| 2378 |
657.2997 | 656.2924 | 656.3170 | -37.4 | 0 | 9 | 0.18 | 1Score > 14 indicates identity |
FFESK |
| 2392 |
331.6670 | 661.3194 | 661.3395 | -30.3 | 0 | 9 | 0.93 | 1Score > 23 indicates identity Score > 22 indicates homology |
SATVER |
| 3470 |
791.8986 | 1581.7826 | 1581.8038 | -13.4 | 0 | 9 | 0.66 | 1Score > 22 indicates identity Score > 20 indicates homology |
TFLEQNLQVYGLR + 2 Deamidated (NQ) |
| 2931 |
361.8318 | 1082.4736 | 1082.4880 | -13.3 | 0 | 9 | 0.47 | 1Score > 18 indicates identity |
QTEDYITGR + Deamidated (NQ) |
| 3415 |
749.9177 | 1497.8208 | 1497.7609 | 40.0 | 0 | 9 | 0.55 | 1Score > 22 indicates identity Score > 19 indicates homology |
NSQILVIMTNHAR + 2 Deamidated (NQ) |
| 2342 |
642.2943 | 641.2870 | 641.2881 | -1.73 | 0 | 9 | 0.12 | 1Score > 13 indicates identity |
DGSAHR |
| 3723 |
646.0198 | 1935.0376 | 1935.0975 | -31.0 | 1 | 9 | 0.28 | 1Score > 22 indicates identity Score > 16 indicates homology |
MVVIDILERLQQLPPR + Oxidation (M) |
| 3655 |
915.4658 | 1828.9170 | 1828.9279 | -5.93 | 1 | 9 | 0.25 | 1Score > 23 indicates identity Score > 16 indicates homology |
GVSSLLDQVNQQVRQR + 3 Deamidated (NQ) |
| 2651 |
447.2528 | 892.4910 | 892.4555 | 39.8 | 0 | 9 | 0.35 | 1Score > 20 indicates identity Score > 17 indicates homology |
SANLFWR |
| 2821 |
508.2722 | 1014.5298 | 1014.4982 | 31.2 | 0 | 9 | 0.24 | 1Score > 23 indicates identity Score > 16 indicates homology |
NGVASNPDLK + Deamidated (NQ) |
| 2869 |
347.8430 | 1040.5072 | 1040.5251 | -17.2 | 0 | 9 | 0.3 | 1Score > 20 indicates identity Score > 16 indicates homology |
HSDNLSVAAK |
| 3249 |
666.8758 | 1331.7370 | 1331.7119 | 18.9 | 0 | 9 | 0.73 | 1Score > 21 indicates identity Score > 20 indicates homology |
ILLMSATLEGER |
| 2303 |
625.4027 | 624.3954 | 624.3707 | 39.6 | 0 | 9 | 0.12 | 1Score > 13 indicates identity |
QPRPK |
| 3045 |
390.8690 | 1169.5852 | 1169.6040 | -16.1 | 1 | 9 | 0.7 | 1Score > 22 indicates identity Score > 20 indicates homology |
KQQPLQQAAR + 3 Deamidated (NQ) |
| 3389 |
738.9017 | 1475.7888 | 1475.7845 | 2.97 | 1 | 9 | 0.23 | 1Score > 22 indicates identity Score > 15 indicates homology |
LRPVQSGGGSRFSK + Deamidated (NQ) |
| 3069 |
601.2949 | 1200.5752 | 1200.6098 | -28.8 | 1 | 9 | 0.52 | 1Score > 22 indicates identity Score > 19 indicates homology |
GANLANQDLRK + 2 Deamidated (NQ) |
| 2093 |
491.2303 | 490.2230 | 490.2136 | 19.3 | 0 | 9 | 0.4 | 1Score > 20 indicates identity Score > 18 indicates homology |
DGGSR |
| 2304 |
313.2054 | 624.3962 | 624.3707 | 40.9 | 0 | 9 | 0.12 | 1Score > 13 indicates identity |
LHLSR |
| 4013 |
1166.1079 | 2330.2012 | 2330.1808 | 8.79 | 1 | 9 | 1 | 1Score > 23 indicates identity Score > 22 indicates homology |
KGATLAFSHGFSIHYNQVVPR + 2 Deamidated (NQ) |
| 2247 |
600.2896 | 599.2823 | 599.2915 | -15.3 | 0 | 9 | 0.22 | 1Score > 15 indicates identity |
LQGNPA + Deamidated (NQ) |
| 2517 |
770.3848 | 769.3775 | 769.4082 | -39.9 | 0 | 9 | 0.39 | 1Score > 17 indicates identity |
LQAPDAR |
| 2943 |
543.7700 | 1085.5254 | 1085.5618 | -33.5 | 0 | 9 | 0.78 | 1Score > 21 indicates identity Score > 20 indicates homology |
QVEWLQQR |
| 2325 |
634.3326 | 633.3253 | 633.3235 | 2.95 | 0 | 9 | 0.43 | 1Score > 21 indicates identity Score > 18 indicates homology |
GFPSAR |
| 3139 |
625.3534 | 1248.6922 | 1248.6826 | 7.69 | 1 | 9 | 0.21 | 1Score > 21 indicates identity Score > 15 indicates homology |
QVKVFSLDTGR |
| 2587 |
418.2184 | 834.4222 | 834.4235 | -1.55 | 0 | 9 | 0.71 | 1Score > 22 indicates identity Score > 20 indicates homology |
YNTAPIR + Deamidated (NQ) |
| 2770 |
495.2875 | 988.5604 | 988.5441 | 16.6 | 0 | 9 | 0.83 | 1Score > 21 indicates identity Score > 21 indicates homology |
QVLATEISK + Deamidated (NQ) |
| 3612 |
591.9916 | 1772.9530 | 1772.9971 | -24.9 | 1 | 9 | 0.51 | 1Score > 22 indicates identity Score > 18 indicates homology |
NQLGLKAVVIFINMGR + Deamidated (NQ) |
| 2783 |
994.5327 | 993.5254 | 993.5719 | -46.8 | 1 | 9 | 0.5 | 1Score > 22 indicates identity Score > 18 indicates homology |
RLGQLHAAK + Deamidated (NQ) |
| 3774 |
692.7131 | 2075.1175 | 2075.0218 | 46.1 | 1 | 9 | 1 | 1Score > 21 indicates identity |
VLIDNLDVSHQPMHGRAR + Oxidation (M); 2 Deamidated (NQ) |
| 2051 |
473.2838 | 472.2765 | 472.2758 | 1.59 | 0 | 9 | 0.73 | 1Score > 23 indicates identity Score > 20 indicates homology |
LAGGR |
| 2637 |
439.2372 | 876.4598 | 876.4817 | -25.0 | 1 | 9 | 1 | 1Score > 21 indicates identity Score > 21 indicates homology |
KQINFAR + Deamidated (NQ) |
| 2886 |
525.2900 | 1048.5654 | 1048.5157 | 47.4 | 1 | 9 | 0.8 | 1Score > 21 indicates identity Score > 20 indicates homology |
RMVEMLNR + Deamidated (NQ) |
| 3310 |
467.2614 | 1398.7624 | 1398.7579 | 3.18 | 1 | 9 | 0.3 | 1Score > 23 indicates identity Score > 16 indicates homology |
DDQGRLLLGSLGR |
| 3426 |
378.6985 | 1510.7649 | 1510.7238 | 27.2 | 1 | 9 | 0.66 | 1Score > 22 indicates identity Score > 19 indicates homology |
EPMELFVDFRGR + Oxidation (M) |
| 2724 |
481.7390 | 961.4634 | 961.4440 | 20.3 | 0 | 9 | 0.84 | 1Score > 22 indicates identity Score > 20 indicates homology |
HMIDAGYR |
| 2767 |
493.2873 | 984.5600 | 984.5240 | 36.6 | 1 | 9 | 0.68 | 1Score > 21 indicates identity Score > 19 indicates homology |
REILDDPK |
| 3996 |
578.5617 | 2310.2177 | 2310.1323 | 37.0 | 1 | 9 | 0.54 | 1Score > 23 indicates identity Score > 18 indicates homology |
GIMLGPGQLFMVDAKATGWMR + 2 Oxidation (M) |
| 2703 |
474.2680 | 946.5214 | 946.5269 | -5.81 | 1 | 9 | 0.72 | 1Score > 22 indicates identity Score > 20 indicates homology |
MKIALQAR + Oxidation (M); Deamidated (NQ) |
| 2399 |
664.3440 | 663.3367 | 663.3340 | 4.08 | 0 | 9 | 0.56 | 1Score > 22 indicates identity Score > 18 indicates homology |
FNLDR |
| 3228 |
653.3339 | 1304.6532 | 1304.6724 | -14.7 | 1 | 8 | 0.26 | 1Score > 23 indicates identity Score > 15 indicates homology |
AFQAQVNEIKR + 2 Deamidated (NQ) |
| 2549 |
805.4300 | 804.4227 | 804.4355 | -15.8 | 1 | 8 | 0.8 | 1Score > 21 indicates identity Score > 20 indicates homology |
RAAGQFR |
| 2245 |
300.1952 | 598.3758 | 598.3802 | -7.30 | 1 | 8 | 0.14 | 1Score > 13 indicates identity |
NKIPK |
| 3623 |
600.3441 | 1798.0105 | 1797.9771 | 18.6 | 1 | 8 | 0.68 | 1Score > 19 indicates identity |
IGVMGAEQAAGVLAQVKR + Deamidated (NQ) |
| 3391 |
370.1986 | 1476.7653 | 1476.8314 | -44.7 | 1 | 8 | 0.46 | 1Score > 23 indicates identity Score > 17 indicates homology |
SVTHLPRVLYHR |
| 2641 |
442.2415 | 882.4684 | 882.4923 | -27.0 | 0 | 8 | 0.49 | 1Score > 20 indicates identity Score > 18 indicates homology |
LGAPLEQR |
| 2707 |
476.7643 | 951.5140 | 951.4960 | 19.0 | 1 | 8 | 0.44 | 1Score > 20 indicates identity Score > 17 indicates homology |
LNCKVYR |
| 3418 |
750.9168 | 1499.8190 | 1499.7692 | 33.2 | 1 | 8 | 0.31 | 1Score > 22 indicates identity Score > 16 indicates homology |
QIAGDLARGNGDVSK |
| 2873 |
349.1480 | 1044.4222 | 1044.4117 | 10.00 | 0 | 8 | 0.19 | 1Score > 14 indicates identity |
MTCGSGFGGR + Oxidation (M) |
| 3212 |
647.8377 | 1293.6608 | 1293.6421 | 14.5 | 1 | 8 | 0.84 | 1Score > 22 indicates identity Score > 20 indicates homology |
VSTREMAEMLK |
| 3284 |
689.8792 | 1377.7438 | 1377.6929 | 37.0 | 0 | 8 | 0.41 | 1Score > 21 indicates identity Score > 17 indicates homology |
APDGLGGELFFQK |
| 3211 |
646.8119 | 1291.6092 | 1291.6628 | -41.5 | 1 | 8 | 1 | 1Score > 21 indicates identity Score > 21 indicates homology |
KMLVAMVDDVR + Oxidation (M) |
| 2202 |
560.2722 | 559.2649 | 559.2714 | -11.6 | 0 | 8 | 0.18 | 1Score > 20 indicates identity Score > 13 indicates homology |
VGNDR |
| 2884 |
524.7572 | 1047.4998 | 1047.5461 | -44.2 | 1 | 8 | 0.82 | 1Score > 22 indicates identity Score > 20 indicates homology |
NIAVDGFRR + Deamidated (NQ) |
| 2972 |
367.1817 | 1098.5233 | 1098.5597 | -33.2 | 0 | 8 | 0.66 | 1Score > 20 indicates identity Score > 19 indicates homology |
YPGDEIVPPL |
| 2451 |
702.4515 | 701.4442 | 701.4323 | 17.0 | 0 | 8 | 0.3 | 1Score > 15 indicates identity |
LELLSK |
| 2814 |
506.2389 | 1010.4632 | 1010.4603 | 2.88 | 1 | 8 | 0.62 | 1Score > 21 indicates identity Score > 19 indicates homology |
MNNRFQGK + Oxidation (M); Deamidated (NQ) |
| 3839 |
734.3915 | 2200.1527 | 2200.1483 | 1.99 | 1 | 8 | 0.24 | 1Score > 22 indicates identity Score > 15 indicates homology |
DKTLEMPVLILPSVQVNMR + Oxidation (M); 2 Deamidated (NQ) |
| 2576 |
411.6937 | 821.3728 | 821.4032 | -36.9 | 0 | 8 | 0.91 | 1Score > 20 indicates identity |
AEGGFVSR |
| 3537 |
853.3661 | 1704.7176 | 1704.6809 | 21.5 | 0 | 8 | 0.42 | 1Score > 17 indicates identity |
ASTSSSSSSSSNQQTEK + 3 Deamidated (NQ) |
| 2755 |
490.7260 | 979.4374 | 979.4723 | -35.6 | 0 | 8 | 0.88 | 1Score > 20 indicates identity |
VATQGYQGR + Deamidated (NQ) |
| 3662 |
461.9789 | 1843.8865 | 1843.9237 | -20.2 | 0 | 8 | 0.55 | 1Score > 23 indicates identity Score > 18 indicates homology |
MLGDGQLDELLEEIIR + Deamidated (NQ) |
| 3992 |
1153.1090 | 2304.2034 | 2304.1148 | 38.5 | 1 | 8 | 0.38 | 1Score > 22 indicates identity Score > 16 indicates homology |
RLFHTSDWHLGQSLHGQER + Deamidated (NQ) |
| 4029 |
585.8196 | 2339.2493 | 2339.2559 | -2.81 | 0 | 8 | 1 | 1Score > 21 indicates identity Score > 20 indicates homology |
LPEEATQIYLGMPAPLAVAALR + Oxidation (M) |
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