MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1789 423.2047 422.1974
1790 423.2373 422.2300
1791 423.2376 422.2303
1792 423.2376 422.2303
1793 423.2379 422.2306
1794 423.2381 422.2308
1795 423.2699 422.2626
1796 423.2938 422.2865
1797 425.0021 423.9948
1798 425.1392 424.1319
1799 425.1583 424.1510
1800 425.1590 424.1517
1801 425.1787 424.1714
1802 425.1788 424.1715
1803 425.1788 424.1715
1804 425.1789 424.1716
1805 425.1790 424.1717
1806 425.1796 424.1723
1807 425.1799 424.1726
1808 425.1802 424.1729
1809 425.2145 424.2072
1810 425.2148 424.2075
1811 425.2151 424.2078
1812 425.2153 424.2080
1813 425.2155 424.2082
1814 425.2157 424.2084
1815 425.2159 424.2086
1816 425.2162 424.2089
1817 425.2163 424.2090
1818 425.2173 424.2100
1819 425.2174 424.2101
1820 426.1776 425.1703
1821 426.2705 425.2632
1822 426.2717 425.2644
1823 427.0121 426.0048
1824 427.1731 426.1658
1825 427.1734 426.1661
1826 427.1942 426.1869
1827 427.1945 426.1872
1828 427.1946 426.1873
1829 427.1960 426.1887
1830 427.2327 426.2254
1831 427.2917 426.2844
1832 429.1453 428.1380
1833 429.1804 428.1731
1834 429.2065 428.1992
1835 429.2833 428.2760
1836 430.2774 429.2701
1837 430.2781 429.2708
1838 431.0648 430.0575
1839 431.0651 430.0578
1840 431.2256 430.2183
1844 432.1136 431.1063
1845 432.2203 431.2130
1846 432.2808 431.2735
1847 433.1834 432.1761
1848 433.1854 432.1781
1849 433.1858 432.1785
1850 433.1858 432.1785
1851 433.2259 432.2186
1852 434.2224 433.2151
1853 434.2415 433.2342
1854 435.1999 434.1926
1855 435.1999 434.1926
1856 435.2000 434.1927
1857 435.2001 434.1928
1858 435.2003 434.1930
1859 435.2003 434.1930
1860 435.2003 434.1930
1861 435.2005 434.1932
1862 435.2008 434.1935
1863 435.2010 434.1937
1864 435.2012 434.1939
1865 435.2014 434.1941
1866 435.2016 434.1943
1867 435.2023 434.1950
1868 435.2026 434.1953
1869 435.2386 434.2313
1870 436.2317 435.2244
1871 437.0858 436.0785
1872 437.0900 436.0827
1873 437.1377 436.1304
1874 437.1790 436.1717
1875 437.1792 436.1719
1876 437.1794 436.1721
1877 437.1795 436.1722
1878 437.1795 436.1722
1879 437.1796 436.1723
1880 437.1801 436.1728
1881 437.1803 436.1730
1882 437.2148 436.2075
1883 437.2154 436.2081
1884 437.2158 436.2085
1885 437.2159 436.2086
1886 437.2159 436.2086
1887 437.2160 436.2087
1888 437.2161 436.2088
1889 437.2164 436.2091
1890 437.2165 436.2092
1891 437.2167 436.2094
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