MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1689 411.1997 410.1924
1690 411.1998 410.1925
1691 411.2000 410.1927
1692 411.2003 410.1930
1693 413.1588 412.1515
1694 413.1796 412.1723
1695 413.1800 412.1727
1696 413.2150 412.2077
1697 413.2163 412.2090
1698 413.2164 412.2091
1699 415.0911 414.0838
1700 415.0935 414.0862
1701 415.1069 414.0996
1702 415.1304 414.1231
1703 415.1549 414.1476
1704 415.1947 414.1874
1705 415.2546 414.2473
1706 416.2618 415.2545
1707 416.2621 415.2548
1708 416.2623 415.2550
1709 416.2628 415.2555
1710 416.2869 415.2796
1711 417.1907 416.1834
1712 417.1907 416.1834
1713 417.1914 416.1841
1714 417.2078 416.2005
1715 417.2460 416.2387
1716 417.2466 416.2393
1717 418.2058 417.1985
1718 419.1694 418.1621
1719 419.1696 418.1623
1720 419.1699 418.1626
1721 419.2050 418.1977
1722 419.2051 418.1978
1723 419.2056 418.1983
1724 419.2057 418.1984
1725 419.2058 418.1985
1726 419.2061 418.1988
1727 419.2064 418.1991
1728 419.2067 418.1994
1729 419.2071 418.1998
1730 419.2083 418.2010
1731 419.2100 418.2027
1732 419.2461 418.2388
1733 420.2494 419.2421
1734 421.0592 420.0519
1735 421.1840 420.1767
1736 421.1841 420.1768
1737 421.1841 420.1768
1738 421.1843 420.1770
1739 421.1844 420.1771
1740 421.1844 420.1771
1741 421.1844 420.1771
1742 421.1845 420.1772
1743 421.1847 420.1774
1744 421.1850 420.1777
1745 421.1853 420.1780
1746 421.1857 420.1784
1747 421.1860 420.1787
1748 421.1864 420.1791
1749 421.1872 420.1799
1750 421.2197 420.2124
1751 421.2202 420.2129
1752 421.2203 420.2130
1753 421.2208 420.2135
1754 421.2209 420.2136
1755 421.2219 420.2146
1756 421.2223 420.2150
1757 421.2223 420.2150
1758 421.2229 420.2156
1759 421.2234 420.2161
1760 421.2237 420.2164
1761 421.2256 420.2183
1762 423.1447 422.1374
1763 423.1631 422.1558
1764 423.1631 422.1558
1765 423.1636 422.1563
1766 423.1638 422.1565
1767 423.1639 422.1566
1768 423.1639 422.1566
1769 423.1642 422.1569
1770 423.1997 422.1924
1771 423.1997 422.1924
1772 423.1998 422.1925
1773 423.2000 422.1927
1774 423.2002 422.1929
1775 423.2002 422.1929
1776 423.2004 422.1931
1777 423.2004 422.1931
1778 423.2004 422.1931
1779 423.2005 422.1932
1780 423.2006 422.1933
1781 423.2007 422.1934
1782 423.2007 422.1934
1783 423.2011 422.1938
1784 423.2014 422.1941
1785 423.2014 422.1941
1786 423.2014 422.1941
1787 423.2016 422.1943
1788 423.2021 422.1948
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