MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1589 403.1748 402.1675
1590 403.2141 402.2068
1591 403.2298 402.2225
1592 403.2303 402.2230
1593 403.2304 402.2231
1594 403.2305 402.2232
1595 403.2307 402.2234
1596 403.2307 402.2234
1597 403.6829 402.6756
1598 405.1532 404.1459
1599 405.1533 404.1460
1600 405.1533 404.1460
1601 405.1896 404.1823
1602 405.1896 404.1823
1603 405.1896 404.1823
1604 405.1899 404.1826
1605 405.1900 404.1827
1606 405.1905 404.1832
1607 405.1905 404.1832
1608 405.1908 404.1835
1609 405.1910 404.1837
1610 405.1910 404.1837
1611 405.1915 404.1842
1612 405.1940 404.1867
1613 405.2099 404.2026
1614 405.2266 404.2193
1615 405.2288 404.2215
1616 405.2302 404.2229
1617 407.1684 406.1611
1618 407.1686 406.1613
1619 407.1686 406.1613
1620 407.1689 406.1616
1621 407.1689 406.1616
1622 407.1692 406.1619
1623 407.1692 406.1619
1624 407.1694 406.1621
1625 407.1694 406.1621
1626 407.1697 406.1624
1627 407.1697 406.1624
1628 407.2048 406.1975
1629 407.2049 406.1976
1630 407.2052 406.1979
1631 407.2053 406.1980
1632 407.2055 406.1982
1633 407.2056 406.1983
1634 407.2056 406.1983
1635 407.2061 406.1988
1636 407.2062 406.1989
1637 407.2063 406.1990
1638 407.2066 406.1993
1639 407.2072 406.1999
1640 407.2077 406.2004
1641 407.2087 406.2014
1642 407.2097 406.2024
1643 407.2436 406.2363
1644 409.0374 408.0301
1645 409.1486 408.1413
1646 409.1643 408.1570
1647 409.1646 408.1573
1648 409.1659 408.1586
1649 409.1832 408.1759
1650 409.1837 408.1764
1651 409.1839 408.1766
1652 409.1841 408.1768
1653 409.1841 408.1768
1654 409.1842 408.1769
1655 409.1843 408.1770
1656 409.1843 408.1770
1657 409.1845 408.1772
1658 409.1846 408.1773
1659 409.1848 408.1775
1660 409.1848 408.1775
1661 409.1850 408.1777
1662 409.1854 408.1781
1663 409.1860 408.1787
1664 409.1860 408.1787
1665 409.2208 408.2135
1666 409.2210 408.2137
1667 409.2210 408.2137
1668 409.2211 408.2138
1669 409.2220 408.2147
1670 409.2221 408.2148
1671 409.2254 408.2181
1672 410.0260 409.0187
1673 410.1965 409.1892
1674 411.0129 410.0056
1675 411.0493 410.0420
1676 411.0704 410.0631
1677 411.1631 410.1558
1678 411.1633 410.1560
1679 411.1634 410.1561
1680 411.1636 410.1563
1681 411.1637 410.1564
1682 411.1637 410.1564
1683 411.1638 410.1565
1684 411.1644 410.1571
1685 411.1991 410.1918
1686 411.1993 410.1920
1687 411.1993 410.1920
1688 411.1995 410.1922
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