MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 4218)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1489 393.2990 392.2917
1490 393.2991 392.2918
1491 393.2991 392.2918
1492 393.2992 392.2919
1493 393.2992 392.2919
1494 393.2993 392.2920
1495 393.2994 392.2921
1496 393.2997 392.2924
1497 393.3001 392.2928
1498 393.3007 392.2934
1499 393.3022 392.2949
1500 393.3026 392.2953
1501 395.0744 394.0671
1502 395.0756 394.0683
1503 395.1072 394.0999
1504 395.1328 394.1255
1505 395.1485 394.1412
1506 395.1682 394.1609
1507 395.1682 394.1609
1508 395.1683 394.1610
1509 395.1683 394.1610
1510 395.1684 394.1611
1511 395.1686 394.1613
1512 395.1686 394.1613
1513 395.1687 394.1614
1514 395.1687 394.1614
1515 395.1688 394.1615
1516 395.1689 394.1616
1517 395.1692 394.1619
1518 395.1694 394.1621
1519 395.1696 394.1623
1520 395.1696 394.1623
1521 395.1697 394.1624
1522 395.1701 394.1628
1523 395.1716 394.1643
1524 395.2040 394.1967
1525 395.2043 394.1970
1526 395.2045 394.1972
1527 395.2045 394.1972
1528 395.2047 394.1974
1529 395.2047 394.1974
1530 395.2048 394.1975
1531 395.2048 394.1975
1532 395.2049 394.1976
1533 395.2050 394.1977
1534 395.2053 394.1980
1535 395.2054 394.1981
1536 395.2055 394.1982
1537 395.2056 394.1983
1538 395.2057 394.1984
1539 395.2066 394.1993
1540 395.2073 394.2000
1541 397.1311 396.1238
1542 397.1479 396.1406
1543 397.1487 396.1414
1544 397.1491 396.1418
1545 397.1836 396.1763
1546 397.1837 396.1764
1547 397.1839 396.1766
1548 397.1840 396.1767
1549 397.1840 396.1767
1550 397.1842 396.1769
1551 397.1845 396.1772
1552 397.1846 396.1773
1553 397.1848 396.1775
1554 397.1848 396.1775
1555 397.1851 396.1778
1556 397.1852 396.1779
1557 397.1853 396.1780
1558 397.1853 396.1780
1559 397.1858 396.1785
1560 397.2204 396.2131
1561 397.2207 396.2134
1562 397.2213 396.2140
1563 397.2223 396.2150
1564 398.2765 397.2692
1565 399.1278 398.1205
1566 399.1337 398.1264
1567 399.1458 398.1385
1568 399.1470 398.1397
1569 399.1642 398.1569
1570 399.1647 398.1574
1571 399.1687 398.1614
1572 399.2001 398.1928
1573 399.2010 398.1937
1574 399.2361 398.2288
1575 400.2311 399.2238
1576 400.2313 399.2240
1577 401.1133 400.1060
1578 401.1595 400.1522
1579 401.1787 400.1714
1580 401.2876 400.2803
1581 401.2878 400.2805
1582 402.2173 401.2100
1583 402.2463 401.2390
1584 403.0701 402.0628
1585 403.1743 402.1670
1586 403.1747 402.1674
1587 403.1747 402.1674
1588 403.1748 402.1675
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