MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1389 387.0998 386.0925
1390 387.1354 386.1281
1391 387.1789 386.1716
1392 387.1821 386.1748
1393 387.1990 386.1917
1394 387.2031 386.1958
1395 388.0975 387.0902
1396 388.1629 387.1556
1397 388.2420 387.2347
1398 388.2545 387.2472
1399 388.2567 387.2494
1400 389.0904 388.0831
1401 389.1587 388.1514
1402 389.1589 388.1516
1403 389.1959 388.1886
1404 389.1963 388.1890
1405 389.1969 388.1896
1406 389.1987 388.1914
1407 389.2172 388.2099
1408 389.2510 388.2437
1409 389.2511 388.2438
1410 389.2524 388.2451
1411 389.2528 388.2455
1412 391.0559 390.0486
1413 391.1380 390.1307
1414 391.1740 390.1667
1415 391.1741 390.1668
1416 391.1742 390.1669
1417 391.1742 390.1669
1418 391.1743 390.1670
1419 391.1743 390.1670
1420 391.1745 390.1672
1421 391.1746 390.1673
1422 391.1748 390.1675
1423 391.1752 390.1679
1424 391.1752 390.1679
1425 391.1755 390.1682
1426 391.1764 390.1691
1427 391.2103 390.2030
1428 391.2105 390.2032
1429 391.2109 390.2036
1430 391.2112 390.2039
1431 391.2113 390.2040
1432 391.2126 390.2053
1433 391.2142 390.2069
1434 391.2142 390.2069
1435 391.2854 390.2781
1436 393.0587 392.0514
1437 393.0726 392.0653
1438 393.1165 392.1092
1439 393.1524 392.1451
1440 393.1526 392.1453
1441 393.1527 392.1454
1442 393.1529 392.1456
1443 393.1529 392.1456
1444 393.1530 392.1457
1445 393.1532 392.1459
1446 393.1535 392.1462
1447 393.1537 392.1464
1448 393.1537 392.1464
1449 393.1539 392.1466
1450 393.1891 392.1818
1451 393.1891 392.1818
1452 393.1894 392.1821
1453 393.1895 392.1822
1454 393.1895 392.1822
1455 393.1895 392.1822
1456 393.1896 392.1823
1457 393.1896 392.1823
1458 393.1896 392.1823
1459 393.1902 392.1829
1460 393.1905 392.1832
1461 393.1906 392.1833
1462 393.1907 392.1834
1463 393.1908 392.1835
1464 393.1910 392.1837
1465 393.1919 392.1846
1466 393.2097 392.2024
1467 393.2298 392.2225
1468 393.2973 392.2900
1469 393.2977 392.2904
1470 393.2978 392.2905
1471 393.2979 392.2906
1472 393.2979 392.2906
1473 393.2979 392.2906
1474 393.2980 392.2907
1475 393.2980 392.2907
1476 393.2980 392.2907
1477 393.2981 392.2908
1478 393.2981 392.2908
1479 393.2982 392.2909
1480 393.2982 392.2909
1481 393.2982 392.2909
1482 393.2985 392.2912
1483 393.2987 392.2914
1484 393.2987 392.2914
1485 393.2989 392.2916
1486 393.2989 392.2916
1487 393.2989 392.2916
1488 393.2990 392.2917
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