MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
888 351.1422 350.1349
889 351.1422 350.1349
890 351.1423 350.1350
891 351.1423 350.1350
892 351.1425 350.1352
893 351.1425 350.1352
894 351.1425 350.1352
895 351.1425 350.1352
896 351.1426 350.1353
897 351.1427 350.1354
898 351.1428 350.1355
899 351.1431 350.1358
900 351.1436 350.1363
901 351.1780 350.1707
902 351.1784 350.1711
903 351.1784 350.1711
904 351.1786 350.1713
905 351.1786 350.1713
906 351.1787 350.1714
907 351.1787 350.1714
908 351.1787 350.1714
909 351.1789 350.1716
910 351.1789 350.1716
911 351.1789 350.1716
912 351.1791 350.1718
913 351.1792 350.1719
914 351.1793 350.1720
915 351.1796 350.1723
916 351.1796 350.1723
917 351.1798 350.1725
918 351.1798 350.1725
919 351.1808 350.1735
920 351.1824 350.1751
921 351.2155 350.2082
922 351.2163 350.2090
923 351.2169 350.2096
924 351.2189 350.2116
925 353.0254 352.0181
926 353.0842 352.0769
927 353.1008 352.0935
928 353.1028 352.0955
929 353.1043 352.0970
930 353.1207 352.1134
931 353.1208 352.1135
932 353.1212 352.1139
933 353.1214 352.1141
934 353.1214 352.1141
935 353.1219 352.1146
936 353.1220 352.1147
937 353.1222 352.1149
938 353.1225 352.1152
939 353.1227 352.1154
940 353.1266 352.1193
941 353.1571 352.1498
942 353.1575 352.1502
943 353.1575 352.1502
944 353.1576 352.1503
945 353.1577 352.1504
946 353.1577 352.1504
947 353.1578 352.1505
948 353.1578 352.1505
949 353.1583 352.1510
950 353.1583 352.1510
951 353.1584 352.1511
952 353.1584 352.1511
953 353.1584 352.1511
954 353.1586 352.1513
955 353.1588 352.1515
956 353.1589 352.1516
957 353.1596 352.1523
958 353.1941 352.1868
959 353.1946 352.1873
960 353.1948 352.1875
961 353.1956 352.1883
962 354.2144 353.2071
963 355.0703 354.0630
964 355.0817 354.0744
965 355.1067 354.0994
966 355.1067 354.0994
967 355.1067 354.0994
968 355.1194 354.1121
969 355.1360 354.1287
970 355.1365 354.1292
971 355.1368 354.1295
972 355.1369 354.1296
973 355.1371 354.1298
974 355.1372 354.1299
975 355.1372 354.1299
976 355.1373 354.1300
977 355.1377 354.1304
978 355.1380 354.1307
979 355.1730 354.1657
980 355.1734 354.1661
981 355.1736 354.1663
982 355.1739 354.1666
983 355.1740 354.1667
984 355.1743 354.1670
985 355.1743 354.1670
986 355.1744 354.1671
987 355.1767 354.1694
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