MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
688 337.1638 336.1565
689 337.1639 336.1566
690 337.1640 336.1567
691 337.1642 336.1569
692 337.1645 336.1572
693 337.1645 336.1572
694 337.1652 336.1579
695 337.1998 336.1925
696 337.2028 336.1955
697 338.0334 337.0261
698 338.0555 337.0482
699 339.0758 338.0685
700 339.1047 338.0974
701 339.1049 338.0976
702 339.1059 338.0986
703 339.1060 338.0987
704 339.1061 338.0988
705 339.1062 338.0989
706 339.1076 338.1003
707 339.1117 338.1044
708 339.1122 338.1049
709 339.1208 338.1135
710 339.1414 338.1341
711 339.1416 338.1343
712 339.1419 338.1346
713 339.1420 338.1347
714 339.1420 338.1347
715 339.1421 338.1348
716 339.1421 338.1348
717 339.1422 338.1349
718 339.1422 338.1349
719 339.1423 338.1350
720 339.1423 338.1350
721 339.1424 338.1351
722 339.1424 338.1351
723 339.1424 338.1351
724 339.1427 338.1354
725 339.1427 338.1354
726 339.1597 338.1524
727 339.1784 338.1711
728 339.1785 338.1712
729 339.1786 338.1713
730 339.1787 338.1714
731 339.1788 338.1715
732 339.1792 338.1719
733 339.1792 338.1719
734 339.1793 338.1720
735 339.1800 338.1727
736 339.1801 338.1728
737 339.1802 338.1729
738 339.1805 338.1732
739 339.1811 338.1738
740 340.1620 339.1547
741 340.2597 339.2524
742 340.2599 339.2526
743 341.1001 340.0928
744 341.1014 340.0941
745 341.1210 340.1137
746 341.1210 340.1137
747 341.1211 340.1138
748 341.1214 340.1141
749 341.1217 340.1144
750 341.1217 340.1144
751 341.1219 340.1146
752 341.1372 340.1299
753 341.1374 340.1301
754 341.1380 340.1307
755 341.1382 340.1309
756 341.1575 340.1502
757 341.1576 340.1503
758 341.1577 340.1504
759 341.1578 340.1505
760 341.1579 340.1506
761 341.1579 340.1506
762 341.1579 340.1506
763 341.1581 340.1508
764 341.1582 340.1509
765 341.1583 340.1510
766 341.1584 340.1511
767 341.1585 340.1512
768 341.1717 340.1644
769 341.1953 340.1880
770 341.1964 340.1891
771 341.1982 340.1909
772 342.9134 341.9061
773 343.0358 342.0285
774 343.1162 342.1089
775 343.1368 342.1295
776 343.1375 342.1302
777 343.1520 342.1447
778 343.1743 342.1670
779 343.2108 342.2035
780 343.2993 342.2920
781 344.2076 343.2003
782 344.2290 343.2217
783 345.0278 344.0205
784 345.1315 344.1242
785 345.1316 344.1243
786 345.1316 344.1243
787 345.1322 344.1249
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