MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
588 333.1684 332.1611
589 333.1684 332.1611
590 333.1685 332.1612
591 333.1685 332.1612
592 333.1686 332.1613
593 333.1690 332.1617
594 333.1690 332.1617
595 333.1690 332.1617
596 333.1696 332.1623
597 333.1698 332.1625
598 333.1712 332.1639
599 333.1885 332.1812
600 333.1887 332.1814
601 333.1891 332.1818
602 333.1894 332.1821
603 333.2074 332.2001
604 333.2136 332.2063
605 334.0091 333.0018
606 334.0400 333.0327
607 334.1508 333.1435
608 335.0146 334.0073
609 335.0307 334.0234
610 335.0901 334.0828
611 335.0902 334.0829
612 335.1103 334.1030
613 335.1104 334.1031
614 335.1109 334.1036
615 335.1112 334.1039
616 335.1112 334.1039
617 335.1113 334.1040
618 335.1114 334.1041
619 335.1120 334.1047
620 335.1126 334.1053
621 335.1469 334.1396
622 335.1469 334.1396
623 335.1470 334.1397
624 335.1471 334.1398
625 335.1471 334.1398
626 335.1472 334.1399
627 335.1472 334.1399
628 335.1476 334.1403
629 335.1476 334.1403
630 335.1477 334.1404
631 335.1477 334.1404
632 335.1479 334.1406
633 335.1479 334.1406
634 335.1479 334.1406
635 335.1480 334.1407
636 335.1480 334.1407
637 335.1483 334.1410
638 335.1484 334.1411
639 335.1490 334.1417
640 335.1493 334.1420
641 335.1834 334.1761
642 335.1836 334.1763
643 335.1838 334.1765
644 335.1841 334.1768
645 335.1847 334.1774
646 335.1847 334.1774
647 335.1848 334.1775
648 335.1849 334.1776
649 335.1851 334.1778
650 335.1852 334.1779
651 335.1853 334.1780
652 335.1858 334.1785
653 335.1869 334.1796
654 335.1869 334.1796
655 335.1975 334.1902
656 335.9657 334.9584
657 337.0331 336.0258
658 337.0358 336.0285
659 337.1060 336.0987
660 337.1068 336.0995
661 337.1257 336.1184
662 337.1261 336.1188
663 337.1262 336.1189
664 337.1262 336.1189
665 337.1262 336.1189
666 337.1263 336.1190
667 337.1263 336.1190
668 337.1266 336.1193
669 337.1267 336.1194
670 337.1268 336.1195
671 337.1268 336.1195
672 337.1269 336.1196
673 337.1276 336.1203
674 337.1625 336.1552
675 337.1625 336.1552
676 337.1627 336.1554
677 337.1628 336.1555
678 337.1628 336.1555
679 337.1628 336.1555
680 337.1631 336.1558
681 337.1633 336.1560
682 337.1633 336.1560
683 337.1634 336.1561
684 337.1634 336.1561
685 337.1635 336.1562
686 337.1635 336.1562
687 337.1636 336.1563
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