MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
488 327.0753 326.0680
489 327.0803 326.0730
490 327.0850 326.0777
491 327.0854 326.0781
492 327.0862 326.0789
493 327.0866 326.0793
494 327.1050 326.0977
495 327.1051 326.0978
496 327.1053 326.0980
497 327.1057 326.0984
498 327.1058 326.0985
499 327.1059 326.0986
500 327.1063 326.0990
501 327.1209 326.1136
502 327.1210 326.1137
503 327.1214 326.1141
504 327.1224 326.1151
505 327.1414 326.1341
506 327.1416 326.1343
507 327.1425 326.1352
508 327.1425 326.1352
509 327.1425 326.1352
510 327.1426 326.1353
511 327.1426 326.1353
512 327.1431 326.1358
513 327.1431 326.1358
514 327.1432 326.1359
515 327.1434 326.1361
516 327.1776 326.1703
517 327.1782 326.1709
518 327.1783 326.1710
519 327.1784 326.1711
520 327.1785 326.1712
521 327.1790 326.1717
522 327.1790 326.1717
523 327.1790 326.1717
524 327.1793 326.1720
525 327.2023 326.1950
526 328.0173 327.0100
527 329.0672 328.0599
528 329.1211 328.1138
529 329.1371 328.1298
530 329.1384 328.1311
531 329.1572 328.1499
532 329.1576 328.1503
533 329.1580 328.1507
534 329.1581 328.1508
535 329.1581 328.1508
536 329.1582 328.1509
537 329.1584 328.1511
538 329.1594 328.1521
539 329.1594 328.1521
540 329.1931 328.1858
541 329.1937 328.1864
542 329.1938 328.1865
543 330.2292 329.2219
544 331.0707 330.0634
545 331.0850 330.0777
546 331.1154 330.1081
547 331.1157 330.1084
548 331.1161 330.1088
549 331.1162 330.1089
550 331.1163 330.1090
551 331.1528 330.1455
552 331.1529 330.1456
553 331.1529 330.1456
554 331.1530 330.1457
555 331.1534 330.1461
556 331.1535 330.1462
557 331.1535 330.1462
558 331.1543 330.1470
559 331.1556 330.1483
560 331.1751 330.1678
561 331.1754 330.1681
562 331.1760 330.1687
563 332.0301 331.0228
564 332.1708 331.1635
565 332.1867 331.1794
566 333.0549 332.0476
567 333.0551 332.0478
568 333.0551 332.0478
569 333.0562 332.0489
570 333.1017 332.0944
571 333.1313 332.1240
572 333.1316 332.1243
573 333.1316 332.1243
574 333.1317 332.1244
575 333.1317 332.1244
576 333.1319 332.1246
577 333.1322 332.1249
578 333.1322 332.1249
579 333.1324 332.1251
580 333.1325 332.1252
581 333.1331 332.1258
582 333.1373 332.1300
583 333.1512 332.1439
584 333.1526 332.1453
585 333.1679 332.1606
586 333.1680 332.1607
587 333.1681 332.1608
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