MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
388 321.1680 320.1607
389 321.1682 320.1609
390 321.1682 320.1609
391 321.1685 320.1612
392 321.1687 320.1614
393 321.1688 320.1615
394 321.1689 320.1616
395 321.1690 320.1617
396 321.1690 320.1617
397 321.1693 320.1620
398 321.1694 320.1621
399 321.1695 320.1622
400 321.1698 320.1625
401 321.1707 320.1634
402 321.2057 320.1984
403 322.0764 321.0691
404 322.9829 321.9756
405 322.9836 321.9763
406 323.0174 322.0101
407 323.0737 322.0664
408 323.1103 322.1030
409 323.1104 322.1031
410 323.1104 322.1031
411 323.1104 322.1031
412 323.1105 322.1032
413 323.1108 322.1035
414 323.1110 322.1037
415 323.1110 322.1037
416 323.1110 322.1037
417 323.1112 322.1039
418 323.1112 322.1039
419 323.1113 322.1040
420 323.1267 322.1194
421 323.1468 322.1395
422 323.1469 322.1396
423 323.1469 322.1396
424 323.1470 322.1397
425 323.1470 322.1397
426 323.1471 322.1398
427 323.1472 322.1399
428 323.1472 322.1399
429 323.1472 322.1399
430 323.1472 322.1399
431 323.1473 322.1400
432 323.1473 322.1400
433 323.1474 322.1401
434 323.1474 322.1401
435 323.1474 322.1401
436 323.1474 322.1401
437 323.1477 322.1404
438 323.1481 322.1408
439 323.1484 322.1411
440 323.1484 322.1411
441 323.1835 322.1762
442 323.1844 322.1771
443 323.1846 322.1773
444 323.1847 322.1774
445 323.1851 322.1778
446 323.1852 322.1779
447 323.1852 322.1779
448 323.1853 322.1780
449 323.1865 322.1792
450 323.9669 322.9596
451 324.9509 323.9436
452 324.9841 323.9768
453 325.0600 324.0527
454 325.0897 324.0824
455 325.0898 324.0825
456 325.0899 324.0826
457 325.0903 324.0830
458 325.1053 324.0980
459 325.1059 324.0986
460 325.1259 324.1186
461 325.1260 324.1187
462 325.1262 324.1189
463 325.1262 324.1189
464 325.1263 324.1190
465 325.1263 324.1190
466 325.1263 324.1190
467 325.1265 324.1192
468 325.1266 324.1193
469 325.1268 324.1195
470 325.1268 324.1195
471 325.1269 324.1196
472 325.1271 324.1198
473 325.1271 324.1198
474 325.1627 324.1554
475 325.1628 324.1555
476 325.1628 324.1555
477 325.1630 324.1557
478 325.1631 324.1558
479 325.1632 324.1559
480 325.1635 324.1562
481 325.1636 324.1563
482 325.1638 324.1565
483 325.1639 324.1566
484 325.1642 324.1569
485 325.1996 324.1923
486 325.2004 324.1931
487 325.2044 324.1971
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