MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4218)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
288 315.1794 314.1721
289 315.1807 314.1734
290 315.1808 314.1735
291 316.1391 315.1318
292 316.1524 315.1451
293 316.1758 315.1685
294 316.1761 315.1688
295 316.1763 315.1690
296 316.1984 315.1911
297 316.1986 315.1913
298 317.0331 316.0258
299 317.1000 316.0927
300 317.1002 316.0929
301 317.1026 316.0953
302 317.1363 316.1290
303 317.1364 316.1291
304 317.1367 316.1294
305 317.1374 316.1301
306 317.1377 316.1304
307 317.1379 316.1306
308 317.1385 316.1312
309 317.1594 316.1521
310 317.1745 316.1672
311 317.1748 316.1675
312 317.1751 316.1678
313 317.1758 316.1685
314 317.1760 316.1687
315 317.1765 316.1692
316 317.1940 316.1867
317 318.1192 317.1119
318 318.1906 317.1833
319 318.1914 317.1841
320 318.1917 317.1844
321 318.1918 317.1845
322 318.3036 317.2963
323 319.0404 318.0331
324 319.0412 318.0339
325 319.0946 318.0873
326 319.1154 318.1081
327 319.1155 318.1082
328 319.1158 318.1085
329 319.1158 318.1085
330 319.1158 318.1085
331 319.1160 318.1087
332 319.1160 318.1087
333 319.1160 318.1087
334 319.1161 318.1088
335 319.1164 318.1091
336 319.1165 318.1092
337 319.1371 318.1298
338 319.1523 318.1450
339 319.1524 318.1451
340 319.1525 318.1452
341 319.1526 318.1453
342 319.1530 318.1457
343 319.1530 318.1457
344 319.1531 318.1458
345 319.1533 318.1460
346 319.1535 318.1462
347 319.1536 318.1463
348 319.1538 318.1465
349 319.1541 318.1468
350 319.1664 318.1591
351 319.1732 318.1659
352 319.1896 318.1823
353 319.1897 318.1824
354 319.1916 318.1843
355 319.9933 318.9860
356 319.9942 318.9869
357 320.0247 319.0174
358 320.1349 319.1276
359 320.1704 319.1631
360 320.1709 319.1636
361 321.0631 320.0558
362 321.0948 320.0875
363 321.0950 320.0877
364 321.0955 320.0882
365 321.0956 320.0883
366 321.1312 320.1239
367 321.1312 320.1239
368 321.1312 320.1239
369 321.1313 320.1240
370 321.1313 320.1240
371 321.1313 320.1240
372 321.1315 320.1242
373 321.1315 320.1242
374 321.1315 320.1242
375 321.1315 320.1242
376 321.1317 320.1244
377 321.1317 320.1244
378 321.1318 320.1245
379 321.1318 320.1245
380 321.1319 320.1246
381 321.1319 320.1246
382 321.1321 320.1248
383 321.1322 320.1249
384 321.1323 320.1250
385 321.1327 320.1254
386 321.1676 320.1603
387 321.1680 320.1607
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