MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
188 309.1684 308.1611
189 309.1686 308.1613
190 309.1686 308.1613
191 309.1690 308.1617
192 309.1694 308.1621
193 309.1695 308.1622
194 309.1712 308.1639
195 309.2060 308.1987
196 309.2072 308.1999
197 310.0135 309.0062
198 310.1122 309.1049
199 311.0564 310.0491
200 311.0743 310.0670
201 311.0893 310.0820
202 311.0895 310.0822
203 311.0914 310.0841
204 311.1104 310.1031
205 311.1104 310.1031
206 311.1104 310.1031
207 311.1106 310.1033
208 311.1107 310.1034
209 311.1107 310.1034
210 311.1107 310.1034
211 311.1108 310.1035
212 311.1111 310.1038
213 311.1118 310.1045
214 311.1118 310.1045
215 311.1468 310.1395
216 311.1471 310.1398
217 311.1472 310.1399
218 311.1473 310.1400
219 311.1473 310.1400
220 311.1474 310.1401
221 311.1474 310.1401
222 311.1474 310.1401
223 311.1476 310.1403
224 311.1478 310.1405
225 311.1479 310.1406
226 311.1479 310.1406
227 311.1480 310.1407
228 311.1485 310.1412
229 311.1489 310.1416
230 311.1831 310.1758
231 311.1845 310.1772
232 311.1849 310.1776
233 311.1888 310.1815
234 312.0536 311.0463
235 313.0587 312.0514
236 313.0600 312.0527
237 313.0604 312.0531
238 313.0605 312.0532
239 313.0904 312.0831
240 313.0906 312.0833
241 313.0963 312.0890
242 313.0967 312.0894
243 313.1060 312.0987
244 313.1063 312.0990
245 313.1070 312.0997
246 313.1264 312.1191
247 313.1264 312.1191
248 313.1264 312.1191
249 313.1267 312.1194
250 313.1270 312.1197
251 313.1271 312.1198
252 313.1628 312.1555
253 313.1631 312.1558
254 313.1634 312.1561
255 313.1638 312.1565
256 313.1642 312.1569
257 313.1643 312.1570
258 313.1646 312.1573
259 313.1648 312.1575
260 313.1651 312.1578
261 313.1652 312.1579
262 313.1654 312.1581
263 313.2006 312.1933
264 314.1834 313.1761
265 314.1969 313.1896
266 314.1974 313.1901
267 315.0534 314.0461
268 315.1061 314.0988
269 315.1118 314.1045
270 315.1129 314.1056
271 315.1199 314.1126
272 315.1209 314.1136
273 315.1209 314.1136
274 315.1217 314.1144
275 315.1218 314.1145
276 315.1420 314.1347
277 315.1423 314.1350
278 315.1424 314.1351
279 315.1425 314.1352
280 315.1431 314.1358
281 315.1438 314.1365
282 315.1439 314.1366
283 315.1445 314.1372
284 315.1446 314.1373
285 315.1573 314.1500
286 315.1595 314.1522
287 315.1789 314.1716
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