MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
88 305.1375 304.1302
89 305.1376 304.1303
90 305.1378 304.1305
91 305.1381 304.1308
92 305.1577 304.1504
93 305.1745 304.1672
94 305.1750 304.1677
95 305.1750 304.1677
96 305.1757 304.1684
97 306.1092 305.1019
98 306.1093 305.1020
99 306.1550 305.1477
100 306.1550 305.1477
101 306.1555 305.1482
102 306.1557 305.1484
103 306.1558 305.1485
104 307.0100 306.0027
105 307.0100 306.0027
106 307.0196 306.0123
107 307.0202 306.0129
108 307.0798 306.0725
109 307.0799 306.0726
110 307.0946 306.0873
111 307.0952 306.0879
112 307.0954 306.0881
113 307.1153 306.1080
114 307.1155 306.1082
115 307.1156 306.1083
116 307.1156 306.1083
117 307.1157 306.1084
118 307.1157 306.1084
119 307.1158 306.1085
120 307.1158 306.1085
121 307.1159 306.1086
122 307.1161 306.1088
123 307.1161 306.1088
124 307.1162 306.1089
125 307.1162 306.1089
126 307.1163 306.1090
127 307.1163 306.1090
128 307.1520 306.1447
129 307.1521 306.1448
130 307.1523 306.1450
131 307.1523 306.1450
132 307.1523 306.1450
133 307.1525 306.1452
134 307.1525 306.1452
135 307.1526 306.1453
136 307.1527 306.1454
137 307.1528 306.1455
138 307.1529 306.1456
139 307.1530 306.1457
140 307.1530 306.1457
141 307.1533 306.1460
142 307.1534 306.1461
143 307.1535 306.1462
144 307.1536 306.1463
145 307.1547 306.1474
146 307.1898 306.1825
147 307.1903 306.1830
148 307.1905 306.1832
149 307.9941 306.9868
150 308.1490 307.1417
151 308.9783 307.9710
152 309.0512 308.0439
153 309.0945 308.0872
154 309.0945 308.0872
155 309.0948 308.0875
156 309.0951 308.0878
157 309.0951 308.0878
158 309.0951 308.0878
159 309.0956 308.0883
160 309.1108 308.1035
161 309.1304 308.1231
162 309.1309 308.1236
163 309.1312 308.1239
164 309.1313 308.1240
165 309.1313 308.1240
166 309.1313 308.1240
167 309.1315 308.1242
168 309.1316 308.1243
169 309.1316 308.1243
170 309.1316 308.1243
171 309.1317 308.1244
172 309.1317 308.1244
173 309.1317 308.1244
174 309.1318 308.1245
175 309.1319 308.1246
176 309.1319 308.1246
177 309.1320 308.1247
178 309.1320 308.1247
179 309.1322 308.1249
180 309.1324 308.1251
181 309.1679 308.1606
182 309.1680 308.1607
183 309.1681 308.1608
184 309.1681 308.1608
185 309.1683 308.1610
186 309.1683 308.1610
187 309.1683 308.1610
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