MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Quantitation overview (109 proteins)

Score Mass Matches Sequences emPAI
Score Mass Matches Sequences emPAI
1 3FLAG_TEV_PP_2683 7938 66327 301 (265) 62 (55) 559.31 Fusion_protein_N_term_3FLAGTEV_in_frame_with_PP_2683
2 Q88JG7_PSEPK 1226 41896 41 (34) 24 (21) 12.26 Alcohol dehydrogenase, iron-containing OS=Pseudomonas putida (strain KT2440) GN=PP_2682 PE=4 SV=1
3 TRYP_PIG 210 25078 7 (6) 4 (4) 1.12 Trypsin OS=Sus scrofa PE=1 SV=1
4 CH60_PSEPK 142 56765 9 (7) 9 (7) 0.48 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
5 Q88M80_PSEPK 82 55707 18 (5) 7 (2) 0.19 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1694 PE=4 SV=1
6 RS4_PSEPK 69 23270 1 (1) 1 (1) 0.14 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1
7 Q88I44_PSEPK 60 28118 2 (2) 1 (1) 0.25 Inositol monophosphatase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3157 PE=4 SV=1
8 Q88IY0_PSEPK 50 71164 6 (2) 4 (1) 0.05 Oxidoreductase, FMN-binding OS=Pseudomonas putida (strain KT2440) GN=PP_2869 PE=4 SV=1
9.1 Q88JT1_PSEPK 46 190223 17 (3) 10 (2) 0.05 Leucine-rich repeat domain protein OS=Pseudomonas putida (strain KT2440) GN=PP_2566 PE=4 SV=1
9.2 Q88BY3_PSEPK 38 50447 2 (2) 2 (2) 0.14 Transposition protein, TnsC-related protein OS=Pseudomonas putida (strain KT2440) GN=PP_5404 PE=4 SV=1
10 Q88L42_PSEPK 44 21645 1 (1) 1 (1) 0.16 Response regulator NasT OS=Pseudomonas putida (strain KT2440) GN=nasT PE=4 SV=1
11 Q88KQ0_PSEPK 44 70282 9 (2) 4 (1) 0.10 ABC efflux transporter, permease/ATP-binding protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2240 PE=3 SV=1
12.1 Q88JM2_PSEPK 43 135367 7 (2) 5 (2) 0.05 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2627 PE=4 SV=1
12.2 Q88RE4_PSEPK 40 0 3 (3) 2 (2) 0.40 description
12.3 KRT84_HUMAN 34 65942 6 (2) 3 (2) 0.10 Keratin, type II cuticular Hb4 OS=Homo sapiens GN=KRT84 PE=2 SV=2
13 Q88E50_PSEPK 43 30420 8 (4) 1 (1) 0.11 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4618 PE=4 SV=1
14 K1H1_HUMAN 42 48633 6 (1) 4 (1) 0.07 Keratin, type I cuticular Ha1 OS=Homo sapiens GN=KRT31 PE=2 SV=3
15 Q88PC1_PSEPK 42 13579 2 (1) 2 (1) 0.25 Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_0929 PE=3 SV=1
16 Q88JV3_PSEPK 40 7216 10 (3) 1 (1) 0.49 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2544 PE=4 SV=1
17 KRA3A_SHEEP 38 15709 1 (1) 1 (1) 0.22 Keratin, high-sulfur matrix protein, IIIA3A OS=Ovis aries PE=1 SV=1
18 Q88ND7_PSEPK 38 17352 3 (1) 2 (1) 0.20 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1275 PE=4 SV=1
19 Q88GM9_PSEPK 38 98841 8 (2) 6 (2) 0.07 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_3692 PE=4 SV=1
20 Q88D01_PSEPK 37 36909 2 (1) 1 (1) 0.09 Proline iminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pip PE=3 SV=1
21 DNAK_PSEPK 36 68873 4 (1) 4 (1) 0.05 Chaperone protein DnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=3 SV=1
22 Q88M23_PSEPK 35 0 3 (2) 3 (2) 0.12 description
23 Q88F78_PSEPK 33 288971 15 (1) 8 (1) 0.01 Non-ribosomal peptide synthetase domain protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4220 PE=4 SV=1
24 Q88L67_PSEPK 33 46116 3 (1) 2 (1) 0.07 Multidrug efflux MFS membrane fusion protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2068 PE=4 SV=1
25 Q88PP4_PSEPK 33 79623 4 (1) 3 (1) 0.04 Protein secretion ABC efflux system, permease and ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_0804 PE=3 SV=1
26 Q88MS2_PSEPK 32 26512 4 (1) 4 (1) 0.13 Transcriptional regulator, TetR family OS=Pseudomonas putida (strain KT2440) GN=PP_1497 PE=4 SV=1
27 Q88IR6_PSEPK 32 31240 3 (1) 2 (1) 0.11 Glutathione S-transferase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_2933 PE=4 SV=1
28.1 Q88NY5_PSEPK 32 28416 4 (2) 4 (2) 0.25 Amino acid ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_1068 PE=3 SV=1
28.2 LOLD_PSEPK 26 0 2 (2) 2 (2) 0.28 description
29 HSCA_PSEPK 32 66019 2 (1) 2 (1) 0.05 Chaperone protein HscA homolog OS=Pseudomonas putida (strain KT2440) GN=hscA PE=3 SV=1
30 SYGA_PSEPK 32 36115 2 (1) 1 (1) 0.09 Glycine--tRNA ligase alpha subunit OS=Pseudomonas putida (strain KT2440) GN=glyQ PE=3 SV=1
31 Q88QF9_PSEPK 31 79219 1 (1) 1 (1) 0.04 Outer membrane ferric siderophore receptor OS=Pseudomonas putida (strain KT2440) GN=PP_0535 PE=3 SV=1
32 RSGA_PSEPK 31 0 4 (1) 3 (1) 0.09 description
33 Q88GG6_PSEPK 31 45874 3 (1) 2 (1) 0.07 Response regulator/sensor histidine kinase OS=Pseudomonas putida (strain KT2440) GN=PP_3758 PE=4 SV=1
34 Q88LF0_PSEPK 30 128606 4 (1) 4 (1) 0.03 Sensory box protein OS=Pseudomonas putida (strain KT2440) GN=PP_1983 PE=4 SV=1
35 Q88Q18_PSEPK 30 89907 7 (2) 3 (1) 0.07 ATP-dependent protease, putative OS=Pseudomonas putida (strain KT2440) GN=PP_0680 PE=4 SV=1
36 Q88R76_PSEPK 30 15606 1 (1) 1 (1) 0.22 LysM domain protein OS=Pseudomonas putida (strain KT2440) GN=PP_0258 PE=4 SV=1
37 Q88H31_PSEPK 30 31933 5 (1) 4 (1) 0.10 Transcriptional regulator, LysR family OS=Pseudomonas putida (strain KT2440) GN=PP_3534 PE=4 SV=1
38 Q88EG1_PSEPK 30 15786 7 (2) 3 (2) 0.48 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4504 PE=4 SV=1
39 Q88QC4_PSEPK 30 178535 6 (1) 3 (1) 0.02 Extracellular protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_0570 PE=4 SV=1
40 Q88LJ7_PSEPK 30 25322 3 (2) 2 (1) 0.13 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1934 PE=4 SV=1
41 Q88CD1_PSEPK 29 0 4 (1) 2 (1) 0.07 description
42 Q88CY6_PSEPK 29 53948 2 (1) 1 (1) 0.06 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_5043 PE=4 SV=1
43 Q88JT4_PSEPK 28 101542 4 (1) 3 (1) 0.03 Antibiotic biosynthesis protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2563 PE=4 SV=1
44 Q88LY2_PSEPK 28 48686 2 (1) 2 (1) 0.07 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1794 PE=4 SV=1
45 Q88IQ9_PSEPK 28 10661 2 (1) 2 (1) 0.32 Antitoxin OS=Pseudomonas putida (strain KT2440) GN=PP_2940 PE=3 SV=1
46 Q88JL1_PSEPK 28 129595 8 (2) 8 (2) 0.05 Cellulose synthase operon C protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2638 PE=4 SV=1
47 Q88IV6_PSEPK 28 131249 14 (2) 5 (1) 0.02 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2893 PE=4 SV=1
48 DEF1_PSEPK 28 19569 1 (1) 1 (1) 0.17 Peptide deformylase 1 OS=Pseudomonas putida (strain KT2440) GN=def1 PE=3 SV=1
49 Q88EE9_PSEPK 27 79958 5 (2) 4 (1) 0.08 ATP-dependent DNA helicase RecQ OS=Pseudomonas putida (strain KT2440) GN=recQ PE=4 SV=1
50 Q88QJ6_PSEPK 27 35063 1 (1) 1 (1) 0.09 L-asparaginase, type I OS=Pseudomonas putida (strain KT2440) GN=ansB PE=4 SV=1
51 Q88L52_PSEPK 27 36176 2 (1) 1 (1) 0.09 Lipase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2083 PE=4 SV=1
52 Q88PE9_PSEPK 27 25644 2 (1) 2 (1) 0.13 DNA-binding response regulator ColR OS=Pseudomonas putida (strain KT2440) GN=colR PE=4 SV=1
53 Q88DJ5_PSEPK 27 42821 1 (1) 1 (1) 0.08 Precorrin-6Y C5,15-methyltransferase (Decarboxylating) OS=Pseudomonas putida (strain KT2440) GN=cobL PE=4 SV=1
54 TRPC_PSEPK 27 30528 3 (1) 2 (1) 0.11 Indole-3-glycerol phosphate synthase OS=Pseudomonas putida (strain KT2440) GN=trpC PE=3 SV=1
55 Q88L17_PSEPK 27 60354 3 (1) 3 (1) 0.05 Methyl-accepting chemotaxis transducer OS=Pseudomonas putida (strain KT2440) GN=PP_2120 PE=4 SV=1
56 Q88IM1_PSEPK 27 14701 1 (1) 1 (1) 0.23 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2978 PE=4 SV=1
57 Q88M86_PSEPK 26 34686 3 (1) 3 (1) 0.10 Transcriptional regulator, LysR family OS=Pseudomonas putida (strain KT2440) GN=PP_1688 PE=4 SV=1
58 Q88GH8_PSEPK 26 54105 3 (1) 2 (1) 0.06 Glycolate oxidase, subunit GlcD OS=Pseudomonas putida (strain KT2440) GN=glcD PE=4 SV=1
59 Q88IQ8_PSEPK 26 28910 2 (1) 2 (1) 0.12 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2941 PE=4 SV=1
60 Q88RG4_PSEPK 26 51085 5 (1) 2 (1) 0.06 HlyD family secretion protein OS=Pseudomonas putida (strain KT2440) GN=PP_0166 PE=4 SV=1
61 Q88RR0_PSEPK 26 38753 2 (1) 1 (1) 0.09 Smf protein OS=Pseudomonas putida (strain KT2440) GN=PP_0069 PE=4 SV=1
62 Q88LF7_PSEPK 26 42280 5 (1) 4 (1) 0.08 HlyD family secretion protein OS=Pseudomonas putida (strain KT2440) GN=PP_1976 PE=4 SV=1
63 Q88PM9_PSEPK 25 21278 8 (1) 2 (1) 0.16 Acetyltransferase, GNAT family OS=Pseudomonas putida (strain KT2440) GN=PP_0820 PE=4 SV=1
64 Q88E45_PSEPK 25 0 1 (1) 1 (1) 0.03 description
65 Q88KW4_PSEPK 25 24860 3 (1) 2 (1) 0.13 3-oxoacyl-(Acyl-carrier-protein) reductase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2175 PE=3 SV=1
66 Q88K56_PSEPK 25 42553 4 (1) 3 (1) 0.08 Cysteine desulfurase OS=Pseudomonas putida (strain KT2440) GN=iscS-2 PE=4 SV=1
67 K2C1_HUMAN 25 66170 4 (1) 3 (1) 0.05 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6
68 Q88IC2_PSEPK 25 38542 2 (1) 1 (1) 0.09 ABC transporter, permease protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3077 PE=3 SV=1
69 ANXA5_HUMAN 25 35971 2 (1) 2 (1) 0.09 Annexin A5 OS=Homo sapiens GN=ANXA5 PE=1 SV=2
70 Q88RE9_PSEPK 25 8675 1 (1) 1 (1) 0.40 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0181 PE=4 SV=1
71 Q88EP4_PSEPK 24 127253 5 (1) 4 (1) 0.03 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4410 PE=4 SV=1
72 PKS12_MYCTU 24 432536 3 (1) 3 (1) 0.01 Mycoketide-CoA synthase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=pks12 PE=1 SV=1
73 Q88DC1_PSEPK 24 57983 2 (1) 2 (1) 0.06 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4906 PE=4 SV=1
74 Q88ET2_PSEPK 24 49256 3 (1) 2 (1) 0.07 Response regulator FleR OS=Pseudomonas putida (strain KT2440) GN=fleR PE=4 SV=1
75 Q88LP7_PSEPK 24 0 7 (1) 7 (1) 0.02 description
76 Q88PE1_PSEPK 23 38528 3 (1) 2 (1) 0.09 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0909 PE=4 SV=1
77 Q88FD9_PSEPK 23 96926 2 (1) 2 (1) 0.03 Histidine kinase OS=Pseudomonas putida (strain KT2440) GN=kdpD PE=4 SV=1
78 Q7WTF3_9ACTN 23 420476 11 (1) 10 (1) 0.01 NanA3 OS=Streptomyces nanchangensis OX=204925 GN=nanA3 PE=4 SV=1
79 Q88G33_PSEPK 23 50229 3 (1) 3 (1) 0.07 Phage DNA helicase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3893 PE=4 SV=1
80 Q88L71_PSEPK 23 38337 4 (1) 4 (1) 0.09 Multidrug efflux RND membrane fusion protein OS=Pseudomonas putida (strain KT2440) GN=PP_2064 PE=4 SV=1
81 RL7_PSEPK 23 12592 1 (1) 1 (1) 0.27 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain KT2440) GN=rplL PE=3 SV=2
82 Q88QZ7_PSEPK 23 101376 3 (2) 3 (2) 0.07 Sensory box protein OS=Pseudomonas putida (strain KT2440) GN=PP_0337 PE=4 SV=1
83 RPOB_PSEPK 22 151468 14 (1) 9 (1) 0.02 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1
84 Q88F84_PSEPK 22 47889 3 (1) 3 (1) 0.07 Aminotransferase, class V OS=Pseudomonas putida (strain KT2440) GN=PP_4214 PE=3 SV=1
85 Q88NE9_PSEPK 22 55085 1 (1) 1 (1) 0.06 Fusaric acid resistance protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1263 PE=3 SV=1
86 Q88IZ2_PSEPK 22 19816 1 (1) 1 (1) 0.17 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2857 PE=4 SV=1
87 Q88J56_PSEPK 22 0 4 (2) 2 (1) 0.08 description
88 PDXH_PSEPK 22 24679 1 (1) 1 (1) 0.14 Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Pseudomonas putida (strain KT2440) GN=pdxH PE=3 SV=1
89 A0A1Q2SRM5_STRLA 21 141468 4 (1) 2 (1) 0.02 Indigoidine synthase OS=Streptomyces lavendulae OX=1914 GN=lbpA PE=4 SV=1
90 Q88NS1_PSEPK 21 58533 1 (1) 1 (1) 0.06 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1134 PE=4 SV=1
91 Q88HP3_PSEPK 21 79974 2 (1) 1 (1) 0.04 Oxidoreductase, large subunit, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3310 PE=4 SV=1
92 Q88CI3_PSEPK 21 18337 1 (1) 1 (1) 0.18 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_5198 PE=4 SV=1
93 NADE_PSEPK 20 29426 2 (1) 1 (1) 0.11 NH(3)-dependent NAD(+) synthetase OS=Pseudomonas putida (strain KT2440) GN=nadE PE=3 SV=1
94 Q88G62_PSEPK 19 40547 4 (3) 2 (1) 0.17 Phage tail protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3863 PE=4 SV=1
95 Q88HU7_PSEPK 18 24082 2 (1) 1 (1) 0.14 Glycosyl transferase, group 2 family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3256 PE=4 SV=1
96 Q88LP6_PSEPK 18 70511 4 (1) 3 (1) 0.05 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1883 PE=4 SV=1
97 Q88HT7_PSEPK 18 40010 3 (1) 1 (1) 0.08 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_3266 PE=4 SV=1
98 Q88KT6_PSEPK 18 15481 1 (1) 1 (1) 0.22 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2203 PE=4 SV=1
99 J5JYK1_BEAB2 17 261176 8 (1) 7 (1) 0.01 Polyketide synthase OS=Beauveria bassiana (strain ARSEF 2860) OX=655819 GN=BBA_03810 PE=4 SV=1
100 Q88FW8_PSEPK 16 47841 1 (1) 1 (1) 0.07 Voltage-gated chloride channel family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3959 PE=4 SV=1
101 Q88EZ5_PSEPK 16 36670 2 (1) 1 (1) 0.09 Periplasmic thiosulfate-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_4305 PE=4 SV=1
102 Q88PB2_PSEPK 15 138455 3 (1) 3 (1) 0.02 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0938 PE=4 SV=1
103 Q88F76_PSEPK 15 37109 1 (1) 1 (1) 0.09 SyrP protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4222 PE=4 SV=1
104 SYP_PSEPK 14 63755 4 (1) 3 (1) 0.05 Proline--tRNA ligase OS=Pseudomonas putida (strain KT2440) GN=proS PE=3 SV=1
105 Q88J48_PSEPK 14 51329 3 (1) 1 (1) 0.06 Aldehyde dehydrogenase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_2801 PE=3 SV=1

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