MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 4218)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2547 803.4284 802.4211 802.3821 48.6 0 24 0.062 +1Score > 24 indicates identity GLDVDQR + Deamidated (NQ)
2200 559.3206 558.3133 558.3125 1.39 0 23 0.11 +1Score > 26 indicates identity INGVR + Deamidated (NQ)
2165 531.3045 530.2972 530.2813 30.1 0 23 0.15 +1Score > 27 indicates identity QGIGR + Deamidated (NQ)
2252 301.6674 601.3202 601.3336 -22.2 0 23 0.06 +1Score > 23 indicates identity LAWGR
2220 575.3156 574.3083 574.3074 1.52 0 22 0.095 +1Score > 25 indicates identity QLSAR + Deamidated (NQ)
2620 858.5053 857.4980 857.4607 43.6 0 22 0.069 +1Score > 23 indicates identity VALVDGQR + Deamidated (NQ)
2774 991.4849 990.4776 990.4982 -20.7 0 21 0.07 +1Score > 22 indicates identity LQSTIGSQR + 2 Deamidated (NQ)
2664 457.7821 913.5496 913.5596 -10.9 0 21 0.058 +1Score > 21 indicates identity LLSAILER
3048 393.5437 1177.6093 1177.6277 -15.7 1 20 0.087 +1Score > 22 indicates identity LNRMFLIDR + Deamidated (NQ)
2802 504.2440 1006.4734 1006.4389 34.3 0 20 0.084 +1Score > 22 indicates identity LGQGQMAER + Oxidation (M); 2 Deamidated (NQ)
2621 429.7567 857.4988 857.4607 44.5 0 20 0.064 +1Score > 23 indicates identity
Score > 20 indicates homology		 VALVDGQR + Deamidated (NQ)
2828 511.2527 1020.4908 1020.5240 -32.5 0 20 0.087 +1Score > 22 indicates identity LQEFLQSR + Deamidated (NQ)
2921 539.2688 1076.5230 1076.5251 -1.88 0 20 0.092 +1Score > 22 indicates identity EFNQGQVVR + Deamidated (NQ)
2186 544.3220 543.3147 543.3129 3.39 0 19 0.087 +1Score > 21 indicates identity AIGQR
2430 687.4056 686.3983 686.4188 -29.8 1 19 0.13 +1Score > 24 indicates identity
Score > 23 indicates homology		 GRVVTR
2625 862.4059 861.3986 861.4127 -16.3 0 19 0.11 +1Score > 22 indicates identity TACVAGAGR
3304 465.2533 1392.7381 1392.6772 43.7 0 19 0.092 +1Score > 21 indicates identity
Score > 21 indicates homology		 LANAYIQQVDEK + 2 Deamidated (NQ)
2499 372.2425 742.4704 742.4701 0.46 0 19 0.12 +1Score > 23 indicates identity LATVALR
2184 544.3214 543.3141 543.3129 2.29 0 19 0.097 +1Score > 21 indicates identity AIGQR
2323 317.6696 633.3246 633.3082 26.0 0 19 0.1 +1Score > 21 indicates identity SNSGLR + Deamidated (NQ)
2221 575.3165 574.3092 574.3075 3.06 0 19 0.25 +1Score > 25 indicates identity QITGR + Deamidated (NQ)
2315 316.1706 630.3266 630.3085 28.8 0 19 0.14 +1Score > 23 indicates identity AGQQAR + Deamidated (NQ)
2589 419.2394 836.4642 836.4439 24.3 1 19 0.068 +1Score > 19 indicates identity HKLMHR + Oxidation (M)
2141 515.3306 514.3233 514.3227 1.17 0 18 0.21 +1Score > 24 indicates identity LAGVR
2359 646.3326 645.3253 645.3235 2.89 0 18 0.24 +1Score > 25 indicates identity DWLGR
2632 435.7686 869.5226 869.5520 -33.8 1 18 0.064 +1Score > 19 indicates identity LMPLLRK
2427 344.1935 686.3724 686.3711 1.91 0 18 0.15 +1Score > 22 indicates identity QIGVDR
2183 544.3105 543.3032 543.3129 -17.8 0 18 0.11 +1Score > 21 indicates identity AAQVR
3091 610.3200 1218.6254 1218.6278 -1.92 0 18 0.2 +1Score > 23 indicates identity LAMLDAATELR + Oxidation (M)
2321 634.2952 633.2879 633.3082 -32.0 0 18 0.1 +1Score > 20 indicates identity VSESGR
2717 478.7388 955.4630 955.4611 2.08 0 18 0.084 +1Score > 19 indicates identity LPEDTPER
3007 566.2739 1130.5332 1130.5356 -2.11 0 18 0.14 +1Score > 22 indicates identity EDIQSVFHR + Deamidated (NQ)
3316 469.2500 1404.7282 1404.7613 -23.6 1 18 0.16 +1Score > 22 indicates identity QVAVLFKDVTER + Deamidated (NQ)
2496 743.4442 742.4369 742.4337 4.30 0 18 0.19 +1Score > 23 indicates identity GLTLSPR
3438 765.9090 1529.8034 1529.7824 13.7 0 18 0.17 -1Score > 23 indicates identity
Score > 22 indicates homology		 GAEEVLVESELVEK
2658 453.7346 905.4546 905.4858 -34.4 0 17 0.13 +1Score > 23 indicates identity
Score > 21 indicates homology		 LLQAFGEK + Deamidated (NQ)
2890 1053.5216 1052.5143 1052.5138 0.48 0 17 0.14 +1Score > 21 indicates identity AFTTQTNLR + 2 Deamidated (NQ)
2973 551.7913 1101.5680 1101.5553 11.6 0 17 0.24 +1Score > 24 indicates identity LNLQAGQINK + 4 Deamidated (NQ)
2622 859.4561 858.4488 858.4487 0.14 0 17 0.23 +1Score > 23 indicates identity GWLLEQI + Deamidated (NQ)
3325 470.5591 1408.6555 1408.6517 2.65 1 17 0.17 +1Score > 22 indicates identity MPHTPSSHSEKR + Oxidation (M)
2584 414.2719 826.5292 826.5388 -11.6 1 17 0.081 +1Score > 19 indicates identity LQLRIGK
2950 1089.5996 1088.5923 1088.5502 38.7 0 17 0.18 +1Score > 22 indicates identity APDFAEQLAK
1843 431.2373 430.2300 430.2288 2.80 0 17 0.12 +1Score > 20 indicates identity GGAAR
2219 575.2935 574.2862 574.3075 -37.0 0 17 0.35 +1Score > 25 indicates identity QITGR + Deamidated (NQ)
2824 509.7354 1017.4562 1017.4978 -40.9 0 17 0.12 +1Score > 20 indicates identity AGVLDSEAEK
2822 508.7600 1015.5054 1015.5410 -35.0 1 17 0.24 +1Score > 23 indicates identity QIGRLSANR + 2 Deamidated (NQ)
2190 547.3010 546.2937 546.2762 32.2 0 17 0.28 +1Score > 24 indicates identity SNIGR + Deamidated (NQ)
1841 431.2369 430.2296 430.2288 1.87 0 17 0.11 +1Score > 20 indicates identity
Score > 20 indicates homology		 GGAAR
2409 672.4050 671.3977 671.4078 -15.1 1 17 0.23 +1Score > 23 indicates identity DRLIR
2232 589.3679 588.3606 588.3595 1.92 0 17 0.11 +1Score > 20 indicates identity LTSIR
2322 317.6692 633.3238 633.3082 24.7 0 17 0.16 +1Score > 21 indicates identity SNSGLR + Deamidated (NQ)
3068 597.8161 1193.6176 1193.6148 2.38 0 17 0.16 +1Score > 21 indicates identity IGLLMDMTLR + 2 Oxidation (M)
2776 496.2471 990.4796 990.4982 -18.7 0 17 0.2 +1Score > 22 indicates identity LQSTIGSQR + 2 Deamidated (NQ)
2998 1124.5577 1123.5504 1123.5622 -10.4 1 17 0.17 +1Score > 21 indicates identity TERFSQSLR + Deamidated (NQ)
2191 547.3010 546.2937 546.2762 32.2 0 17 0.29 +1Score > 24 indicates identity SNIGR + Deamidated (NQ)
1842 431.2371 430.2298 430.2288 2.33 0 17 0.13 +1Score > 20 indicates identity GGAAR
2231 589.3677 588.3604 588.3595 1.58 0 17 0.12 +1Score > 20 indicates identity LTSIR
2601 842.4994 841.4921 841.4658 31.3 0 16 0.16 +1Score > 21 indicates identity VTEPALGR
3409 747.8579 1493.7012 1493.7613 -40.2 0 16 0.18 +1Score > 21 indicates identity ISQLNGSINIFSAK + 3 Deamidated (NQ)
2203 560.2733 559.2660 559.2714 -9.65 0 16 0.12 +1Score > 20 indicates identity
Score > 19 indicates homology		 VGNDR
4187 821.7569 2462.2489 2462.2111 15.3 1 16 0.22 +1Score > 22 indicates identity LPSIGGVNLSQMGNAQGAAEKQFK + Oxidation (M); 2 Deamidated (NQ)
2672 464.2854 926.5562 926.5913 -37.8 1 16 0.11 +1Score > 20 indicates identity
Score > 19 indicates homology		 KVLAELVR
2628 434.2113 866.4080 866.4102 -2.50 1 16 0.15 +1Score > 20 indicates identity MIRMER + 2 Oxidation (M)
2980 556.3298 1110.6450 1110.6549 -8.90 0 16 0.086 +1Score > 18 indicates identity AILNALPPFR
2580 413.2647 824.5148 824.4868 34.0 0 16 0.053 +1Score > 16 indicates identity QLPLAQR
3157 630.8183 1259.6220 1259.5993 18.0 0 16 0.25 +1Score > 22 indicates identity ASIQNEADNGLK + Deamidated (NQ)
2088 488.2834 487.2761 487.2754 1.41 0 16 0.43 +1Score > 25 indicates identity NAGVK
2675 464.7897 927.5648 927.5250 43.0 1 16 0.13 +1Score > 20 indicates identity QRLLNQR + Deamidated (NQ)
2408 672.3688 671.3615 671.3602 1.95 0 16 0.15 +1Score > 20 indicates identity NLTPAR + Deamidated (NQ)
2143 516.3147 515.3074 515.3180 -20.5 1 16 0.48 +1Score > 25 indicates identity GKGVR
3168 633.3239 1264.6332 1264.6420 -6.92 1 16 0.23 +1Score > 23 indicates identity
Score > 22 indicates homology		 IAHYMKAIMR + 2 Oxidation (M)
2879 1045.5906 1044.5833 1044.5451 36.6 0 16 0.26 +1Score > 22 indicates identity EELGELLSR
2414 338.1827 674.3508 674.3460 7.24 1 16 0.33 +1Score > 23 indicates identity GSRAER
2891 527.2838 1052.5530 1052.5978 -42.5 0 15 0.13 +1Score > 21 indicates identity
Score > 19 indicates homology		 LVANPQLAAR + Deamidated (NQ)
2849 516.2245 1030.4344 1030.4791 -43.4 1 15 0.062 +1Score > 16 indicates identity QRNEEQAR + Deamidated (NQ)
2367 650.3268 649.3195 649.3217 -3.40 0 15 0.19 +1Score > 21 indicates identity ALGMSR + Oxidation (M)
2987 372.8530 1115.5372 1115.5645 -24.5 0 15 0.24 +1Score > 22 indicates identity SMPPEILSAR + Oxidation (M)
2636 877.3998 876.3925 876.4123 -22.6 0 15 0.15 +1Score > 19 indicates identity MLNAEQR + Oxidation (M)
3009 377.8687 1130.5843 1130.5567 24.4 0 15 0.24 +1Score > 23 indicates identity
Score > 21 indicates homology		 LQSEQQQLR + 2 Deamidated (NQ)
2850 516.2561 1030.4976 1030.4791 18.0 1 15 0.29 +1Score > 22 indicates identity QRNEEQAR + Deamidated (NQ)
2807 505.2301 1008.4456 1008.4699 -24.0 0 15 0.13 +1Score > 19 indicates identity GAMPFDLSR + Oxidation (M)
2657 452.2138 902.4130 902.4570 -48.7 1 15 0.18 +1Score > 20 indicates identity NDTLRER
2258 607.2637 606.2564 606.2795 -38.1 0 15 0.12 +1Score > 18 indicates identity SMVDR
3545 569.9738 1706.8996 1706.8886 6.42 1 15 0.27 +1Score > 22 indicates identity
Score > 22 indicates homology		 LHMQVPGAGLRQVQR + Oxidation (M); 2 Deamidated (NQ)
2626 432.2477 862.4808 862.4661 17.1 0 15 0.14 +1Score > 20 indicates identity
Score > 19 indicates homology		 AGLLNGYR
3093 612.3296 1222.6446 1222.6492 -3.72 0 15 0.2 +1Score > 22 indicates identity
Score > 20 indicates homology		 QIINLHTMPR + Deamidated (NQ)
2556 812.4004 811.3931 811.3824 13.2 0 15 0.1 +1Score > 17 indicates identity DHAQDVK
2853 516.7612 1031.5078 1031.5400 -31.1 0 15 0.081 +1Score > 23 indicates identity
Score > 16 indicates homology		 AVQQYGIPR + Deamidated (NQ)
3358 480.5455 1438.6147 1438.6432 -19.8 0 15 0.13 +1Score > 18 indicates identity QMSPMSAEATVVR + 2 Oxidation (M); Deamidated (NQ)
2197 557.3666 556.3593 556.3697 -18.6 0 15 0.22 +1Score > 20 indicates identity LAVVR
2509 755.4672 754.4599 754.4337 34.7 0 15 0.09 +1Score > 17 indicates identity AQAPLAGK
2647 445.7291 889.4436 889.4141 33.2 0 15 0.34 +1Score > 22 indicates identity QANQALSR + 3 Deamidated (NQ)
2665 458.7239 915.4332 915.4297 3.82 0 15 0.16 +1Score > 21 indicates identity
Score > 19 indicates homology		 LEQPSQGR + 2 Deamidated (NQ)
2743 487.2411 972.4676 972.5128 -46.4 0 14 0.28 +1Score > 22 indicates identity
Score > 21 indicates homology		 LIDAIDQGK + Deamidated (NQ)
2649 445.7352 889.4558 889.4505 6.05 1 14 0.36 +1Score > 23 indicates identity ETAANAKGK + Deamidated (NQ)
2293 622.2623 621.2550 621.2541 1.55 0 14 0.063 +1Score > 15 indicates identity GEGGMR + Oxidation (M)
2536 395.1914 788.3682 788.4028 -43.8 1 14 0.35 +1Score > 22 indicates identity NQIQRK + 3 Deamidated (NQ)
3252 669.3466 1336.6786 1336.6809 -1.67 1 14 0.32 +1Score > 22 indicates identity
Score > 22 indicates homology		 LMEARNQFLAK + Oxidation (M); Deamidated (NQ)
2754 490.2467 978.4788 978.4440 35.6 0 14 0.22 +1Score > 22 indicates identity
Score > 20 indicates homology		 ELEQMASR + Oxidation (M)
2410 336.7123 671.4100 671.4078 3.29 1 14 0.26 +1Score > 23 indicates identity
Score > 21 indicates homology		 RIEVR
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