MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 91–100 (out of 105)


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+91

Accession Score Description
1 Q88HP3_PSEPK 21 Oxidoreductase, large subunit, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3310 PE=4 SV=1

+92

Accession Score Description
1 Q88CI3_PSEPK 21 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_5198 PE=4 SV=1

+93

Accession Score Description
1 NADE_PSEPK 20 NH(3)-dependent NAD(+) synthetase OS=Pseudomonas putida (strain KT2440) GN=nadE PE=3 SV=1

+94

Accession Score Description
1 Q88G62_PSEPK 19 Phage tail protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3863 PE=4 SV=1

+95

Accession Score Description
1 Q88HU7_PSEPK 18 Glycosyl transferase, group 2 family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3256 PE=4 SV=1

+96

Accession Score Description
1 Q88LP6_PSEPK 18 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1883 PE=4 SV=1

+97

Accession Score Description
1 Q88HT7_PSEPK 18 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_3266 PE=4 SV=1

+98

Accession Score Description
1 Q88KT6_PSEPK 18 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2203 PE=4 SV=1

-99

Accession Score Description
1 J5JYK1_BEAB2 17 Polyketide synthase OS=Beauveria bassiana (strain ARSEF 2860) OX=655819 GN=BBA_03810 PE=4 SV=1
Score Mass Matches Sequences emPAI
99.1 J5JYK1_BEAB2 17 261176 8 (1) 7 (1) 0.01
Polyketide synthase OS=Beauveria bassiana (strain ARSEF 2860) OX=655819 GN=BBA_03810 PE=4 SV=1

-8 peptide matches (8 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2162   530.3309 529.3236 529.2972 49.9 0 8 0.95 +1Score > 25 indicates identity
Score > 20 indicates homology
R.AAVNR.K
2360   647.3150 646.3077 646.3286 -32.3 0 3 1.7 +7Score > 22 indicates identity
Score > 18 indicates homology
U R.GGIASNK.V + Deamidated (NQ)
2380   657.4045 656.3972 656.3969 0.45 0 17 0.1 +6Score > 19 indicates identity U R.AILANR.L
2584   414.2719 826.5292 826.5025 32.4 1 9 0.55 +2Score > 19 indicates identity U K.RLGPGVTK.F
2591   838.5389 837.5316 837.5324 -0.90 1 17 0.019 +1Score > 13 indicates identity U K.LVTKLPAP.-
2620   858.5053 857.4980 857.4858 14.2 0 16 0.29 +3Score > 23 indicates identity U K.ALVLEEGK.D
2621   429.7567 857.4988 857.4858 15.2 0 6 1.5 +3Score > 23 indicates identity
Score > 20 indicates homology
U K.ALVLEEGK.D
3214   647.8670 1293.7194 1293.7405 -16.2 1 1 1 +1Score > 20 indicates identity
Score > 13 indicates homology
U K.GRAVVALEPPASK.W

+100

Accession Score Description
1 Q88FW8_PSEPK 16 Voltage-gated chloride channel family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3959 PE=4 SV=1
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