MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 105)

Page: 1 2 3 4 5 6 … 11 Next 

+1

Accession Score Description
1 3FLAG_TEV_PP_2683 7938 Fusion_protein_N_term_3FLAGTEV_in_frame_with_PP_2683

+2

Accession Score Description
1 Q88JG7_PSEPK 1226 Alcohol dehydrogenase, iron-containing OS=Pseudomonas putida (strain KT2440) GN=PP_2682 PE=4 SV=1

+3

Accession Score Description
1 TRYP_PIG 210 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 CH60_PSEPK 142 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

+5

Accession Score Description
1 Q88M80_PSEPK 82 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1694 PE=4 SV=1

+6

Accession Score Description
1 RS4_PSEPK 69 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1

+7

Accession Score Description
1 Q88I44_PSEPK 60 Inositol monophosphatase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3157 PE=4 SV=1

+8

Accession Score Description
1 Q88IY0_PSEPK 50 Oxidoreductase, FMN-binding OS=Pseudomonas putida (strain KT2440) GN=PP_2869 PE=4 SV=1

-9

Accession Score Description
Family member distances as a dendrogram 1 Q88JT1_PSEPK 46 Leucine-rich repeat domain protein OS=Pseudomonas putida (strain KT2440) GN=PP_2566 PE=4 SV=1
2 Q88BY3_PSEPK 38 Transposition protein, TnsC-related protein OS=Pseudomonas putida (strain KT2440) GN=PP_5404 PE=4 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
Q88JT1_PSEPK 46 190223 17 (3) 10 (2) 0.05
Leucine-rich repeat domain protein OS=Pseudomonas putida (strain KT2440) GN=PP_2566 PE=4 SV=1
Q88BY3_PSEPK 38 50447 2 (2) 2 (2) 0.14
Transposition protein, TnsC-related protein OS=Pseudomonas putida (strain KT2440) GN=PP_5404 PE=4 SV=1

-18 peptide matches (14 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
2395 +1 664.3417 663.3344 663.3374 -4.44 0 11 0.63 +2Score > 22 indicates identity U X R.LMSASR.R
2397   332.6746 663.3346 663.3374 -4.11 0 11 0.5 +1Score > 22 indicates identity
Score > 20 indicates homology		 U X R.LMSASR.R
2445   350.2295 698.4444 698.4187 36.8 1 1 2.1 +8Score > 17 indicates identity U X R.SAPRLR.E
2521   775.4313 774.4240 774.4500 -33.6 1 17 0.24 +10Score > 23 indicates identity U X R.FRSPLR.L
2687   938.4938 937.4865 937.5093 -24.3 1 12 0.41 +5Score > 20 indicates identity U X R.QEAGKHLR.S
2746   974.4813 973.4740 973.4828 -9.05 0 31 0.0084 +3Score > 23 indicates identity U X R.LAQSAEAQR.D + Deamidated (NQ)
2747 +2 487.7444 973.4742 973.4828 -8.82 0 27 0.025 +3Score > 23 indicates identity U X R.LAQSAEAQR.D + Deamidated (NQ)
2796   502.2475 1002.4804 1002.5280 -47.4 1 7 0.66 +1Score > 22 indicates identity
Score > 17 indicates homology		 U X R.LEGLQMRR.L + Deamidated (NQ)
2797   502.2567 1002.4988 1002.5280 -29.1 1 27 0.019 +1Score > 23 indicates identity U X -.MNRIIEAR.F + Deamidated (NQ)
2827 +1 510.2441 1018.4736 1018.5229 -48.4 1 14 0.32 +2Score > 21 indicates identity U X R.LEGLQMRR.L + Oxidation (M); Deamidated (NQ)
D:\Xcalibur\Data\Jennifer\PRT1487 Andy\Andy2683a.raw

Score > 23 indicates identity

Score > 15 indicates homology
3661   615.6342 1843.8808 1843.9098 -15.7 0 6 0.42 -1Score > 23 indicates identity
Score > 15 indicates homology		 U X R.LQLDNQTQQIINCVR.H + 2 Deamidated (NQ)
-15.7 0 6 0.42 1 X R.LQLDNQTQQIINCVR.H + 2 Deamidated (NQ)
-15.7 0 6 0.42 1 X R.LQLDNQTQQIINCVR.H + 2 Deamidated (NQ)
-15.7 0 6 0.42 1 X R.LQLDNQTQQIINCVR.H + 2 Deamidated (NQ)
-41.6 1 2 1.1 5 LGAMQVQRQADALSALR   + Oxidation (M); Deamidated (NQ)
-41.6 1 2 1.1 5 LGAMQVQRQADALSALR   + Oxidation (M); Deamidated (NQ)
-15.7 0 2 1.1 7 X R.LQLDNQTQQIINCVR.H + 2 Deamidated (NQ)
-15.7 0 2 1.1 7 X R.LQLDNQTQQIINCVR.H + 2 Deamidated (NQ)
-2.09 1 2 1.2 9 DSRDIEGELRPCIER  
3739   1001.5269 2001.0392 2001.1371 -48.9 1 2 1 +2Score > 23 indicates identity
Score > 14 indicates homology		 U X R.RAQAAGLPPPALLIDTLQR.L + Deamidated (NQ)
4259   639.5519 2554.1785 2554.2883 -43.0 1 1 1 +2Score > 21 indicates identity
Score > 14 indicates homology		 U X K.EMVTMTTPTPLHQNQITRSLPK.W + 2 Oxidation (M)

1 subset or intersection (3 subset proteins in total)

Score Mass Subset of
Q88E57_PSEPK 34 0 9.1
description
2 samesets of Q88E57_PSEPK
Q88PD3_PSEPK 34 0
description
Q88JK4_PSEPK 34 0
description

+10

Accession Score Description
1 Q88L42_PSEPK 44 Response regulator NasT OS=Pseudomonas putida (strain KT2440) GN=nasT PE=4 SV=1
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