MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 105)


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+81

Accession Score Description
1 RL7_PSEPK 23 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain KT2440) GN=rplL PE=3 SV=2

+82

Accession Score Description
1 Q88QZ7_PSEPK 23 Sensory box protein OS=Pseudomonas putida (strain KT2440) GN=PP_0337 PE=4 SV=1

+83

Accession Score Description
1 RPOB_PSEPK 22 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1

-84

Accession Score Description
1 Q88F84_PSEPK 22 Aminotransferase, class V OS=Pseudomonas putida (strain KT2440) GN=PP_4214 PE=3 SV=1
Score Mass Matches Sequences emPAI
84.1 Q88F84_PSEPK 22 47889 3 (1) 3 (1) 0.07
Aminotransferase, class V OS=Pseudomonas putida (strain KT2440) GN=PP_4214 PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2299   312.6770 623.3394 623.3503 -17.5 0 22 0.01 +1Score > 15 indicates identity U R.LAQHR.A
2997   561.3045 1120.5944 1120.5989 -3.94 1 7 1.7 +10Score > 21 indicates identity U R.NILAYRSQR.D + Deamidated (NQ)
3760   1028.0304 2054.0462 2054.0354 5.28 0 1 1 +1Score > 23 indicates identity
Score > 14 indicates homology
U R.QIALTGSTTEGLGMIYGGLR.L + Oxidation (M); Deamidated (NQ)

+85

Accession Score Description
1 Q88NE9_PSEPK 22 Fusaric acid resistance protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1263 PE=3 SV=1

+86

Accession Score Description
1 Q88IZ2_PSEPK 22 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2857 PE=4 SV=1

+87

Accession Score Description
1 Q88J56_PSEPK 22 description

+88

Accession Score Description
1 PDXH_PSEPK 22 Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Pseudomonas putida (strain KT2440) GN=pdxH PE=3 SV=1

+89

Accession Score Description
1 A0A1Q2SRM5_STRLA 21 Indigoidine synthase OS=Streptomyces lavendulae OX=1914 GN=lbpA PE=4 SV=1

+90

Accession Score Description
1 Q88NS1_PSEPK 21 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1134 PE=4 SV=1
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