MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2683a |
| MS data file | : | Andy2683a.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 2 Aug 2026 at 00:54:18 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,770 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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81| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL7_PSEPK | 23 | 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain KT2440) GN=rplL PE=3 SV=2 |
82| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88QZ7_PSEPK | 23 | Sensory box protein OS=Pseudomonas putida (strain KT2440) GN=PP_0337 PE=4 SV=1 |
83| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPK | 22 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 83.1 | RPOB_PSEPK |
22 | 151468 | 14 (1) | 9 (1) | 0.02 | |
| DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1 | |||||||
14 peptide matches (11 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 2218 |
573.3383 | 572.3310 | 572.3282 | 4.93 | 0 | 7 | 1.6 | 4Score > 25 indicates identity Score > 21 indicates homology |
U | K.AQVVR.I + Deamidated (NQ) | |
| 2234 |
1 |
591.3105 | 590.3032 | 590.3024 | 1.42 | 0 | 11 | 0.68 | 4Score > 22 indicates identity |
K.DTSLR.V | |
| 2504 |
374.7274 | 747.4402 | 747.4531 | -17.1 | 0 | 1 | 2.1 | 4Score > 17 indicates identity |
U | R.LIIFDK.E | |
| 2633 |
435.7742 | 869.5338 | 869.5447 | -12.5 | 1 | 3 | 1.9 | 4Score > 19 indicates identity |
U | K.VAKAQVVR.I | |
| 2673 |
927.5637 | 926.5564 | 926.5661 | -10.5 | 1 | 16 | 0.13 | 2Score > 20 indicates identity |
U | R.VGLVRVER.A | |
| 2837 |
1027.5902 | 1026.5829 | 1026.5822 | 0.71 | 0 | 9 | 0.89 | 6Score > 21 indicates identity |
U | R.VSALGPGGLTR.E | |
| 2839 |
2 |
514.2996 | 1026.5846 | 1026.5822 | 2.39 | 0 | 15 | 0.21 | 2Score > 21 indicates identity |
U | R.VSALGPGGLTR.E |
| 2926 |
541.2579 | 1080.5012 | 1080.5101 | -8.19 | 0 | 9 | 0.64 | 2Score > 20 indicates identity |
U | R.DVHPTHYGR.V | |
| 3051 |
592.3491 | 1182.6836 | 1182.6833 | 0.29 | 1 | 22 | 0.027 | 1Score > 19 indicates identity |
U | R.RVSALGPGGLTR.E | |
| 3052 |
395.2356 | 1182.6850 | 1182.6833 | 1.41 | 1 | 9 | 0.53 | 2Score > 19 indicates identity |
U | R.RVSALGPGGLTR.E | |
| 3467 |
525.5972 | 1573.7698 | 1573.7923 | -14.3 | 1 | 5 | 1 | 1Score > 23 indicates identity Score > 18 indicates homology |
U | K.GVPMATPVFDGAKER.E |
84| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88F84_PSEPK | 22 | Aminotransferase, class V OS=Pseudomonas putida (strain KT2440) GN=PP_4214 PE=3 SV=1 |
85| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NE9_PSEPK | 22 | Fusaric acid resistance protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_1263 PE=3 SV=1 |
86| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88IZ2_PSEPK | 22 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2857 PE=4 SV=1 |
87| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88J56_PSEPK | 22 | description |
88| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PDXH_PSEPK | 22 | Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Pseudomonas putida (strain KT2440) GN=pdxH PE=3 SV=1 |
89| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | A0A1Q2SRM5_STRLA | 21 | Indigoidine synthase OS=Streptomyces lavendulae OX=1914 GN=lbpA PE=4 SV=1 |
90| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NS1_PSEPK | 21 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1134 PE=4 SV=1 |
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