MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 105)

Page: 1 2 3 4 5 6 … 11 Next 

+1

Accession Score Description
1 3FLAG_TEV_PP_2683 7938 Fusion_protein_N_term_3FLAGTEV_in_frame_with_PP_2683

+2

Accession Score Description
1 Q88JG7_PSEPK 1226 Alcohol dehydrogenase, iron-containing OS=Pseudomonas putida (strain KT2440) GN=PP_2682 PE=4 SV=1

+3

Accession Score Description
1 TRYP_PIG 210 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 CH60_PSEPK 142 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

+5

Accession Score Description
1 Q88M80_PSEPK 82 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1694 PE=4 SV=1

+6

Accession Score Description
1 RS4_PSEPK 69 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1

+7

Accession Score Description
1 Q88I44_PSEPK 60 Inositol monophosphatase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3157 PE=4 SV=1

-8

Accession Score Description
1 Q88IY0_PSEPK 50 Oxidoreductase, FMN-binding OS=Pseudomonas putida (strain KT2440) GN=PP_2869 PE=4 SV=1
Score Mass Matches Sequences emPAI
8.1 Q88IY0_PSEPK 50 71164 6 (2) 4 (1) 0.05
Oxidoreductase, FMN-binding OS=Pseudomonas putida (strain KT2440) GN=PP_2869 PE=4 SV=1
3 samesets of Q88IY0_PSEPK
Q88KN3_PSEPK 50 0 4 (2) 3 (1) 0.06
description
PVDQ_PSEPK 50 0 6 (2) 4 (1) 0.04
description
Q88PQ5_PSEPK 50 0 3 (2) 2 (1) 0.03
description

-6 peptide matches (5 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2226 +1 587.3521 586.3448 586.3438 1.67 0 38 0.0023 +1Score > 24 indicates identity U R.ALVER.E
2547   803.4284 802.4211 802.3821 48.6 0 9 1.9 +6Score > 24 indicates identity U R.GQVEDVR.A + Deamidated (NQ)
2973   551.7913 1101.5680 1101.5675 0.53 0 13 0.72 +4Score > 24 indicates identity U R.IMIAGGGPAGMK.A
2974   368.1967 1101.5683 1101.5675 0.74 0 12 0.51 +1Score > 24 indicates identity
Score > 22 indicates homology		 U R.IMIAGGGPAGMK.A
D:\Xcalibur\Data\Jennifer\PRT1487 Andy\Andy2683a.raw

Score > 20 indicates identity
-34.6 1 14 0.28 1 REVPTYVMQQR   + Oxidation (M); Deamidated (NQ)
3334   712.3289 1422.6432 1422.6851 -29.4 1 11 0.51 -2Score > 20 indicates identity U R.RSEFGGASTNLQR.E + Deamidated (NQ)
-34.6 1 7 1.3 3 REVPTYVMQQR   + Oxidation (M); Deamidated (NQ)
-32.3 1 7 1.3 4 FSRLDEPFQQR   + Deamidated (NQ)
-29.4 1 7 1.3 4 KHPGQLSGGQQQR   + 3 Deamidated (NQ)
19.3 1 0 6 6 MENDANGASASGKR   + Oxidation (M)

+9

Accession Score Description
Family member distances as a dendrogram 1 Q88JT1_PSEPK 46 Leucine-rich repeat domain protein OS=Pseudomonas putida (strain KT2440) GN=PP_2566 PE=4 SV=1
2 Q88BY3_PSEPK 38 Transposition protein, TnsC-related protein OS=Pseudomonas putida (strain KT2440) GN=PP_5404 PE=4 SV=1

+10

Accession Score Description
1 Q88L42_PSEPK 44 Response regulator NasT OS=Pseudomonas putida (strain KT2440) GN=nasT PE=4 SV=1
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