MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2683a |
| MS data file | : | Andy2683a.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 2 Aug 2026 at 00:54:18 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,770 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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71| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88EP4_PSEPK | 24 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4410 PE=4 SV=1 |
72| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PKS12_MYCTU | 24 | Mycoketide-CoA synthase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=pks12 PE=1 SV=1 |
73| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88DC1_PSEPK | 24 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4906 PE=4 SV=1 |
74| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88ET2_PSEPK | 24 | Response regulator FleR OS=Pseudomonas putida (strain KT2440) GN=fleR PE=4 SV=1 |
75| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88LP7_PSEPK | 24 | description |
76| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PE1_PSEPK | 23 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0909 PE=4 SV=1 |
77| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88FD9_PSEPK | 23 | Histidine kinase OS=Pseudomonas putida (strain KT2440) GN=kdpD PE=4 SV=1 |
78| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q7WTF3_9ACTN | 23 | NanA3 OS=Streptomyces nanchangensis OX=204925 GN=nanA3 PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 78.1 | Q7WTF3_9ACTN |
23 | 420476 | 11 (1) | 10 (1) | 0.01 | |
| NanA3 OS=Streptomyces nanchangensis OX=204925 GN=nanA3 PE=4 SV=1 | |||||||
11 peptide matches (10 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2091 |
489.2434 | 488.2361 | 488.2343 | 3.74 | 0 | 11 | 0.71 | 4Score > 22 indicates identity |
U | R.DAGAR.R | |
| 2160 |
1 |
529.3467 | 528.3394 | 528.3384 | 2.01 | 0 | 14 | 0.31 | 6Score > 22 indicates identity |
R.LVAAR.G | |
| 2215 |
572.3058 | 571.2985 | 571.3078 | -16.2 | 0 | 8 | 0.46 | 1Score > 17 indicates identity |
U | R.ASAPAR.L | |
| 2330 |
640.3187 | 639.3114 | 639.3201 | -13.5 | 1 | 1 | 1.6 | 1Score > 16 indicates identity |
U | R.RNGHR.V + Deamidated (NQ) | |
| 2606 |
422.2690 | 842.5234 | 842.5086 | 17.6 | 1 | 11 | 0.47 | 4Score > 20 indicates identity |
U | R.LLARGASR.L | |
| 2644 |
444.2383 | 886.4620 | 886.4621 | -0.0034 | 1 | 23 | 0.054 | 1Score > 23 indicates identity |
U | R.QLRDAGAR.R + Deamidated (NQ) | |
| 2682 |
468.7128 | 935.4110 | 935.4573 | -49.4 | 0 | 5 | 1.7 | 10Score > 20 indicates identity |
U | R.AAQAEHPGR.I | |
| 2743 |
487.2411 | 972.4676 | 972.5101 | -43.6 | 1 | 14 | 0.33 | 4Score > 22 indicates identity Score > 21 indicates homology |
U | R.QRGLAADSR.C | |
| 2760 |
491.2607 | 980.5068 | 980.5080 | -1.14 | 0 | 1 | 2 | 3Score > 20 indicates identity Score > 17 indicates homology |
U | R.YGPAFTGLR.T | |
| 3001 |
564.7742 | 1127.5338 | 1127.5571 | -20.6 | 0 | 6 | 1.9 | 4Score > 21 indicates identity |
U | R.EALATDPQQR.L |
79| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88G33_PSEPK | 23 | Phage DNA helicase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3893 PE=4 SV=1 |
80| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88L71_PSEPK | 23 | Multidrug efflux RND membrane fusion protein OS=Pseudomonas putida (strain KT2440) GN=PP_2064 PE=4 SV=1 |
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