MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 105)


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+71

Accession Score Description
1 Q88EP4_PSEPK 24 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4410 PE=4 SV=1

+72

Accession Score Description
1 PKS12_MYCTU 24 Mycoketide-CoA synthase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=pks12 PE=1 SV=1

+73

Accession Score Description
1 Q88DC1_PSEPK 24 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4906 PE=4 SV=1

+74

Accession Score Description
1 Q88ET2_PSEPK 24 Response regulator FleR OS=Pseudomonas putida (strain KT2440) GN=fleR PE=4 SV=1

+75

Accession Score Description
1 Q88LP7_PSEPK 24 description

-76

Accession Score Description
1 Q88PE1_PSEPK 23 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0909 PE=4 SV=1
Score Mass Matches Sequences emPAI
76.1 Q88PE1_PSEPK 23 38528 3 (1) 2 (1) 0.09
Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0909 PE=4 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2200   559.3206 558.3133 558.3125 1.39 0 21 0.16 +4Score > 26 indicates identity U K.NLVGR.Q + Deamidated (NQ)
3014   568.3090 1134.6034 1134.5669 32.2 1 8 1 +1Score > 21 indicates identity
Score > 20 indicates homology
U K.ARADFLNAQK.A + 2 Deamidated (NQ)
3015   379.2086 1134.6040 1134.5669 32.7 1 23 0.035 +1Score > 21 indicates identity U K.ARADFLNAQK.A + 2 Deamidated (NQ)

+77

Accession Score Description
1 Q88FD9_PSEPK 23 Histidine kinase OS=Pseudomonas putida (strain KT2440) GN=kdpD PE=4 SV=1

+78

Accession Score Description
1 Q7WTF3_9ACTN 23 NanA3 OS=Streptomyces nanchangensis OX=204925 GN=nanA3 PE=4 SV=1

+79

Accession Score Description
1 Q88G33_PSEPK 23 Phage DNA helicase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3893 PE=4 SV=1

+80

Accession Score Description
1 Q88L71_PSEPK 23 Multidrug efflux RND membrane fusion protein OS=Pseudomonas putida (strain KT2440) GN=PP_2064 PE=4 SV=1
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