MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 105)


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+71

Accession Score Description
1 Q88EP4_PSEPK 24 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4410 PE=4 SV=1

+72

Accession Score Description
1 PKS12_MYCTU 24 Mycoketide-CoA synthase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=pks12 PE=1 SV=1

+73

Accession Score Description
1 Q88DC1_PSEPK 24 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4906 PE=4 SV=1

+74

Accession Score Description
1 Q88ET2_PSEPK 24 Response regulator FleR OS=Pseudomonas putida (strain KT2440) GN=fleR PE=4 SV=1

-75

Accession Score Description
1 Q88LP7_PSEPK 24 description
Score Mass Matches Sequences emPAI
75.1 Q88LP7_PSEPK 24 0 7 (1) 7 (1) 0.02
description
3 samesets of Q88LP7_PSEPK
Q88L55_PSEPK 24 187556 4 (1) 4 (1) 0.02
Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2080 PE=4 SV=1
Q88K55_PSEPK 24 0 2 (1) 2 (1) 0.10
description
TRMD_PSEPK 24 0 3 (1) 1 (1) 0.12
description

-7 peptide matches (7 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2165   531.3045 530.2972 530.2813 30.1 0 18 0.47 +4Score > 27 indicates identity R.DALGR.S
2233   591.3099 590.3026 590.3024 0.41 0 24 0.034 +1Score > 21 indicates identity U R.STVQR.R + Deamidated (NQ)
2585   831.4612 830.4539 830.4498 5.00 0 23 0.069 +4Score > 24 indicates identity U K.DDLLLSR.T
2624   861.3969 860.3896 860.4141 -28.4 0 16 0.18 +7Score > 21 indicates identity U R.GGQPAFQR.T + Deamidated (NQ)
2636   877.3998 876.3925 876.4301 -42.9 0 6 1.1 +3Score > 19 indicates identity U K.TTTPASSGR.G
2667   459.2477 916.4808 916.4978 -18.5 0 5 1.1 +2Score > 24 indicates identity
Score > 18 indicates homology
U R.TLQVGGVSR.L + Deamidated (NQ)
2908   532.8115 1063.6084 1063.5695 36.6 1 3 3.1 +4Score > 20 indicates identity U K.TLSELKMAR.Q + Oxidation (M)

+76

Accession Score Description
1 Q88PE1_PSEPK 23 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0909 PE=4 SV=1

+77

Accession Score Description
1 Q88FD9_PSEPK 23 Histidine kinase OS=Pseudomonas putida (strain KT2440) GN=kdpD PE=4 SV=1

+78

Accession Score Description
1 Q7WTF3_9ACTN 23 NanA3 OS=Streptomyces nanchangensis OX=204925 GN=nanA3 PE=4 SV=1

+79

Accession Score Description
1 Q88G33_PSEPK 23 Phage DNA helicase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3893 PE=4 SV=1

+80

Accession Score Description
1 Q88L71_PSEPK 23 Multidrug efflux RND membrane fusion protein OS=Pseudomonas putida (strain KT2440) GN=PP_2064 PE=4 SV=1
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