MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2683a |
| MS data file | : | Andy2683a.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 2 Aug 2026 at 00:54:18 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,770 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 3FLAG_TEV_PP_2683 | 7938 | Fusion_protein_N_term_3FLAGTEV_in_frame_with_PP_2683 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG7_PSEPK | 1226 | Alcohol dehydrogenase, iron-containing OS=Pseudomonas putida (strain KT2440) GN=PP_2682 PE=4 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 210 | Trypsin OS=Sus scrofa PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 142 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88M80_PSEPK | 82 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1694 PE=4 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS4_PSEPK | 69 | 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88I44_PSEPK | 60 | Inositol monophosphatase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3157 PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 7.1 | Q88I44_PSEPK |
60 | 28118 | 2 (2) | 1 (1) | 0.25 | |
| Inositol monophosphatase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3157 PE=4 SV=1 | |||||||
2 peptide matches (2 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2489 |
730.4106 | 729.4033 | 729.4021 | 1.72 | 0 | 42 | 0.00087 | 1Score > 23 indicates identity |
U | R.ELIQAR.Y + Deamidated (NQ) | |
| 2490 |
365.7100 | 729.4054 | 729.4021 | 4.63 | 0 | 42 | 0.00083 | 1Score > 23 indicates identity |
U | R.ELIQAR.Y + Deamidated (NQ) |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88IY0_PSEPK | 50 | Oxidoreductase, FMN-binding OS=Pseudomonas putida (strain KT2440) GN=PP_2869 PE=4 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JT1_PSEPK | 46 | Leucine-rich repeat domain protein OS=Pseudomonas putida (strain KT2440) GN=PP_2566 PE=4 SV=1 | |
| 2 | Q88BY3_PSEPK | 38 | Transposition protein, TnsC-related protein OS=Pseudomonas putida (strain KT2440) GN=PP_5404 PE=4 SV=1 | |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88L42_PSEPK | 44 | Response regulator NasT OS=Pseudomonas putida (strain KT2440) GN=nasT PE=4 SV=1 |
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