MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 105)


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+61

Accession Score Description
1 Q88RR0_PSEPK 26 Smf protein OS=Pseudomonas putida (strain KT2440) GN=PP_0069 PE=4 SV=1

+62

Accession Score Description
1 Q88LF7_PSEPK 26 HlyD family secretion protein OS=Pseudomonas putida (strain KT2440) GN=PP_1976 PE=4 SV=1

+63

Accession Score Description
1 Q88PM9_PSEPK 25 Acetyltransferase, GNAT family OS=Pseudomonas putida (strain KT2440) GN=PP_0820 PE=4 SV=1

+64

Accession Score Description
1 Q88E45_PSEPK 25 description

+65

Accession Score Description
1 Q88KW4_PSEPK 25 3-oxoacyl-(Acyl-carrier-protein) reductase, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2175 PE=3 SV=1

-66

Accession Score Description
1 Q88K56_PSEPK 25 Cysteine desulfurase OS=Pseudomonas putida (strain KT2440) GN=iscS-2 PE=4 SV=1
Score Mass Matches Sequences emPAI
66.1 Q88K56_PSEPK 25 42553 4 (1) 3 (1) 0.08
Cysteine desulfurase OS=Pseudomonas putida (strain KT2440) GN=iscS-2 PE=4 SV=1

-4 peptide matches (4 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2447   700.4346 699.4273 699.4279 -0.83 0 25 0.017 +1Score > 20 indicates identity U K.LAIDLR.T
2489   730.4106 729.4033 729.4021 1.72 0 21 0.11 +6Score > 23 indicates identity U R.LEQLAR.R + Deamidated (NQ)
2490   365.7100 729.4054 729.4021 4.63 0 19 0.17 +7Score > 23 indicates identity U R.LEQLAR.R + Deamidated (NQ)
3528   848.4434 1694.8722 1694.8774 -3.02 1 2 1.6 +5Score > 22 indicates identity
Score > 16 indicates homology
U K.QRIPHTLNLCIDSK.G + Deamidated (NQ)

+67

Accession Score Description
1 K2C1_HUMAN 25 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6

+68

Accession Score Description
1 Q88IC2_PSEPK 25 ABC transporter, permease protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_3077 PE=3 SV=1

+69

Accession Score Description
1 ANXA5_HUMAN 25 Annexin A5 OS=Homo sapiens GN=ANXA5 PE=1 SV=2

+70

Accession Score Description
1 Q88RE9_PSEPK 25 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0181 PE=4 SV=1
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