MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 105)


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+41

Accession Score Description
1 Q88CD1_PSEPK 29 description

+42

Accession Score Description
1 Q88CY6_PSEPK 29 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_5043 PE=4 SV=1

+43

Accession Score Description
1 Q88JT4_PSEPK 28 Antibiotic biosynthesis protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2563 PE=4 SV=1

+44

Accession Score Description
1 Q88LY2_PSEPK 28 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1794 PE=4 SV=1

+45

Accession Score Description
1 Q88IQ9_PSEPK 28 Antitoxin OS=Pseudomonas putida (strain KT2440) GN=PP_2940 PE=3 SV=1

-46

Accession Score Description
1 Q88JL1_PSEPK 28 Cellulose synthase operon C protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2638 PE=4 SV=1
Score Mass Matches Sequences emPAI
46.1 Q88JL1_PSEPK 28 129595 8 (2) 8 (2) 0.05
Cellulose synthase operon C protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2638 PE=4 SV=1

-8 peptide matches (8 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2165   531.3045 530.2972 530.2813 30.1 0 18 0.47 +4Score > 27 indicates identity R.DALGR.L
2278   617.3265 616.3192 616.3180 1.97 0 27 0.028 +4Score > 24 indicates identity U R.LQQAR.L + 2 Deamidated (NQ)
2520   386.2287 770.4428 770.4511 -10.7 1 23 0.033 +1Score > 21 indicates identity U R.RLQQAR.L
2886   525.2900 1048.5654 1048.5301 33.7 0 5 2 +4Score > 21 indicates identity
Score > 20 indicates homology
U R.QQAQFASLR.R + Deamidated (NQ)
2983   557.7675 1113.5204 1113.5753 -49.3 0 0 1 +2Score > 20 indicates identity
Score > 13 indicates homology
U R.VGVTLMHWR.H + Oxidation (M)
3001   564.7742 1127.5338 1127.5319 1.71 1 2 4.9 +10Score > 21 indicates identity U R.DPGARDAAAQR.E + Deamidated (NQ)
3166   632.3596 1262.7046 1262.6659 30.7 0 1 1 +2Score > 20 indicates identity
Score > 13 indicates homology
U R.QTLAGWLFAEK.R
4190   822.0967 2463.2683 2463.1994 28.0 1 2 0.74 +1Score > 22 indicates identity
Score > 14 indicates homology
U -.MQRMIGVLMLAAITCQADAQPK.D + Oxidation (M); 2 Deamidated (NQ)

1 subset or intersection (8 subset proteins in total)

Score Mass Subset of
HUTH_PSEPK 27 54113 46.1
Histidine ammonia-lyase OS=Pseudomonas putida (strain KT2440) GN=hutH PE=3 SV=1
7 samesets of HUTH_PSEPK
Q88LA7_PSEPK 27 0
description
Q88N21_PSEPK 27 0
description
THIC_PSEPK 27 0
description
Q88CJ4_PSEPK 27 0
description
Q88H01_PSEPK 27 0
description
Q88I36_PSEPK 27 0
description
Q88LD5_PSEPK 27 0
description

+47

Accession Score Description
1 Q88IV6_PSEPK 28 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2893 PE=4 SV=1

+48

Accession Score Description
1 DEF1_PSEPK 28 Peptide deformylase 1 OS=Pseudomonas putida (strain KT2440) GN=def1 PE=3 SV=1

+49

Accession Score Description
1 Q88EE9_PSEPK 27 ATP-dependent DNA helicase RecQ OS=Pseudomonas putida (strain KT2440) GN=recQ PE=4 SV=1

+50

Accession Score Description
1 Q88QJ6_PSEPK 27 L-asparaginase, type I OS=Pseudomonas putida (strain KT2440) GN=ansB PE=4 SV=1
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