MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 105)

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+1

Accession Score Description
1 3FLAG_TEV_PP_2683 7938 Fusion_protein_N_term_3FLAGTEV_in_frame_with_PP_2683

+2

Accession Score Description
1 Q88JG7_PSEPK 1226 Alcohol dehydrogenase, iron-containing OS=Pseudomonas putida (strain KT2440) GN=PP_2682 PE=4 SV=1

+3

Accession Score Description
1 TRYP_PIG 210 Trypsin OS=Sus scrofa PE=1 SV=1

-4

Accession Score Description
1 CH60_PSEPK 142 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
Score Mass Matches Sequences emPAI
4.1 CH60_PSEPK 142 56765 9 (7) 9 (7) 0.48
60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

-9 peptide matches (9 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2087   487.3256 486.3183 486.3166 3.60 0 33 0.0049 +1Score > 22 indicates identity U K.VAAVK.A
2476   360.1779 718.3412 718.3398 1.95 0 41 0.00069 +1Score > 21 indicates identity U K.APGFGDR.R
2668   461.2561 920.4976 920.4967 0.99 0 16 0.04 +1Score > 22 indicates identity
Score > 14 indicates homology		 U K.SFGAPTITK.D
D:\Xcalibur\Data\Jennifer\PRT1487 Andy\Andy2683a.raw

Score > 20 indicates identity
1.66 0 24 0.022 1 ALLNLVER   (match in 74)
-10.5 1 16 0.13 2 VGLVRVER  
-37.6 1 15 0.16 3 KVLAELVR  
2673   927.5637 926.5564 926.5800 -25.5 0 14 0.19 -4Score > 20 indicates identity U R.ALAAIIDLK.G
40.9 0 10 0.57 5 AIPTAALNR   + Deamidated (NQ)
40.9 1 9 0.59 6 DLAPKVQR   + Deamidated (NQ)
24.4 0 9 0.73 7 GWPVISLR  
1.64 0 7 1.1 8 TAGPILSLR  
-10.5 1 6 1.2 9 GRILLEAR  
40.9 1 6 1.3 10 KLAPVDQR   + Deamidated (NQ)
2815   337.8461 1010.5165 1010.5145 1.96 0 44 0.0003 +1Score > 21 indicates identity U R.VEDALHATR.A
3120   413.5586 1237.6540 1237.6527 1.02 1 23 0.038 +1Score > 21 indicates identity U K.ARVEDALHATR.A
3315   469.2364 1404.6874 1404.6853 1.45 1 36 0.00045 +1Score > 23 indicates identity
Score > 15 indicates homology		 U K.AVAAGMNPMDLKR.G + 2 Oxidation (M)
4108   801.4235 2401.2487 2401.1973 21.4 0 20 0.094 +1Score > 22 indicates identity U K.ANDAAGDGTTTATVLAQAIVNEGLK.A + Deamidated (NQ)
4360   917.8403 2750.4991 2750.4636 12.9 0 64 1.8e-006 +1Score > 19 indicates identity U K.AGRPLLIVAEDVEGEALATLVVNNMR.G + Deamidated (NQ)

+5

Accession Score Description
1 Q88M80_PSEPK 82 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1694 PE=4 SV=1

+6

Accession Score Description
1 RS4_PSEPK 69 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1

+7

Accession Score Description
1 Q88I44_PSEPK 60 Inositol monophosphatase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3157 PE=4 SV=1

+8

Accession Score Description
1 Q88IY0_PSEPK 50 Oxidoreductase, FMN-binding OS=Pseudomonas putida (strain KT2440) GN=PP_2869 PE=4 SV=1

+9

Accession Score Description
Family member distances as a dendrogram 1 Q88JT1_PSEPK 46 Leucine-rich repeat domain protein OS=Pseudomonas putida (strain KT2440) GN=PP_2566 PE=4 SV=1
2 Q88BY3_PSEPK 38 Transposition protein, TnsC-related protein OS=Pseudomonas putida (strain KT2440) GN=PP_5404 PE=4 SV=1

+10

Accession Score Description
1 Q88L42_PSEPK 44 Response regulator NasT OS=Pseudomonas putida (strain KT2440) GN=nasT PE=4 SV=1
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