MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2683a |
| MS data file | : | Andy2683a.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 2 Aug 2026 at 00:54:18 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,770 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | 3FLAG_TEV_PP_2683 | 7938 | Fusion_protein_N_term_3FLAGTEV_in_frame_with_PP_2683 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG7_PSEPK | 1226 | Alcohol dehydrogenase, iron-containing OS=Pseudomonas putida (strain KT2440) GN=PP_2682 PE=4 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 210 | Trypsin OS=Sus scrofa PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 3.1 | TRYP_PIG |
210 | 25078 | 7 (6) | 4 (4) | 1.12 | |
| Trypsin OS=Sus scrofa PE=1 SV=1 | |||||||
7 peptide matches (7 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2603 |
842.5103 | 841.5030 | 841.5022 | 1.04 | 0 | 18 | 0.11 | 1Score > 21 indicates identity |
U | R.VATVSLPR.S | |
| 2604 |
421.7589 | 841.5032 | 841.5022 | 1.30 | 0 | 50 | 6.2e-005 | 1Score > 21 indicates identity |
U | R.VATVSLPR.S | |
| 2877 |
523.2878 | 1044.5610 | 1044.5564 | 4.50 | 0 | 54 | 4.5e-005 | 1Score > 23 indicates identity Score > 23 indicates homology |
U | K.LSSPATLNSR.V | |
| 2881 |
523.7784 | 1045.5422 | 1045.5404 | 1.81 | 0 | 52 | 5.2e-005 | 1Score > 24 indicates identity Score > 21 indicates homology |
U | K.LSSPATLNSR.V + Deamidated (NQ) | |
| 3857 |
738.0423 | 2211.1051 | 2211.0807 | 11.0 | 0 | 56 | 2.4e-005 | 1Score > 23 indicates identity |
U | R.LGEHNIDVLEGNEQFINAAK.I + Deamidated (NQ) | |
| 3951 |
762.0668 | 2283.1786 | 2283.1569 | 9.50 | 0 | 56 | 2.1e-005 | 1Score > 22 indicates identity |
U | K.IITHPNFNGNTLDNDIMLIK.L + Deamidated (NQ) | |
| 3954 |
762.3952 | 2284.1638 | 2284.1409 | 10.0 | 0 | 52 | 6.4e-005 | 1Score > 23 indicates identity |
U | K.IITHPNFNGNTLDNDIMLIK.L + 2 Deamidated (NQ) |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 142 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88M80_PSEPK | 82 | Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1694 PE=4 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS4_PSEPK | 69 | 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88I44_PSEPK | 60 | Inositol monophosphatase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3157 PE=4 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88IY0_PSEPK | 50 | Oxidoreductase, FMN-binding OS=Pseudomonas putida (strain KT2440) GN=PP_2869 PE=4 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JT1_PSEPK | 46 | Leucine-rich repeat domain protein OS=Pseudomonas putida (strain KT2440) GN=PP_2566 PE=4 SV=1 | |
| 2 | Q88BY3_PSEPK | 38 | Transposition protein, TnsC-related protein OS=Pseudomonas putida (strain KT2440) GN=PP_5404 PE=4 SV=1 | |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88L42_PSEPK | 44 | Response regulator NasT OS=Pseudomonas putida (strain KT2440) GN=nasT PE=4 SV=1 |
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