MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2683a |
| MS data file | : | Andy2683a.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 2 Aug 2026 at 00:54:18 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Oxidation (M), Deamidated (NQ) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,770 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 36 | Chaperone protein DnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=3 SV=1 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88M23_PSEPK | 35 | description |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88F78_PSEPK | 33 | Non-ribosomal peptide synthetase domain protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4220 PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 23.1 | Q88F78_PSEPK |
33 | 288971 | 15 (1) | 8 (1) | 0.01 | |
| Non-ribosomal peptide synthetase domain protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4220 PE=4 SV=1 | |||||||
| 2 samesets of Q88F78_PSEPK | |||||||
| Q88EQ4_PSEPK |
33 | 0 | 2 (1) | 2 (1) | 0.07 | ||
| description | |||||||
| Q88HK1_PSEPK |
33 | 0 | 1 (1) | 1 (1) | 0.08 | ||
| description | |||||||
15 peptide matches (10 non-duplicate, 5 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2159 |
529.3466 | 528.3393 | 528.3384 | 1.82 | 0 | 33 | 0.0039 | 1Score > 22 indicates identity |
U | K.AVIAR.H | |
| 2556 |
812.4004 | 811.3931 | 811.3534 | 48.9 | 0 | 9 | 0.36 | 2Score > 17 indicates identity |
U | R.QGFDMAK.E + Oxidation (M) | |
| 2698 |
2 |
473.7461 | 945.4776 | 945.4515 | 27.6 | 0 | 6 | 3 | 7Score > 23 indicates identity |
U | R.SENLQAER.D |
| 2702 |
946.4858 | 945.4785 | 945.4515 | 28.5 | 0 | 1 | 9.5 | 5Score > 23 indicates identity |
U | R.SENLQAER.D | |
| 2824 |
509.7354 | 1017.4562 | 1017.4727 | -16.1 | 0 | 5 | 1.8 | 7Score > 20 indicates identity |
U | R.EQSAQVAQR.A + 2 Deamidated (NQ) | |
| 2878 |
523.2988 | 1044.5830 | 1044.5451 | 36.3 | 0 | 6 | 1.2 | 2Score > 22 indicates identity Score > 19 indicates homology |
R.IELGEIESR.L | ||
| 3029 |
575.3549 | 1148.6952 | 1148.6778 | 15.2 | 1 | 1 | 1 | 1Score > 13 indicates identity |
U | K.AVIARHDVLR.T | |
| 3120 |
413.5586 | 1237.6540 | 1237.6891 | -28.4 | 0 | 2 | 4.7 | 6Score > 21 indicates identity |
U | R.ALANAGERPALR.A | |
| 3635 |
1 |
603.3149 | 1806.9229 | 1806.8496 | 40.5 | 1 | 1 | 6.7 | 7Score > 23 indicates identity Score > 22 indicates homology |
U | R.QLDDYARSENLQAER.D |
| 3636 |
2 |
904.4688 | 1806.9230 | 1806.8496 | 40.6 | 1 | 12 | 0.65 | 4Score > 23 indicates identity |
U | R.QLDDYARSENLQAER.D |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88L67_PSEPK | 33 | Multidrug efflux MFS membrane fusion protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2068 PE=4 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88PP4_PSEPK | 33 | Protein secretion ABC efflux system, permease and ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_0804 PE=3 SV=1 |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88MS2_PSEPK | 32 | Transcriptional regulator, TetR family OS=Pseudomonas putida (strain KT2440) GN=PP_1497 PE=4 SV=1 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88IR6_PSEPK | 32 | Glutathione S-transferase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_2933 PE=4 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88NY5_PSEPK | 32 | Amino acid ABC transporter, ATP-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_1068 PE=3 SV=1 | |
| 2 | LOLD_PSEPK | 26 | description | |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HSCA_PSEPK | 32 | Chaperone protein HscA homolog OS=Pseudomonas putida (strain KT2440) GN=hscA PE=3 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYGA_PSEPK | 32 | Glycine--tRNA ligase alpha subunit OS=Pseudomonas putida (strain KT2440) GN=glyQ PE=3 SV=1 |
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