MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 105)

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+11

Accession Score Description
1 Q88KQ0_PSEPK 44 ABC efflux transporter, permease/ATP-binding protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2240 PE=3 SV=1

+12

Accession Score Description
Family member distances as a dendrogram 1 Q88JM2_PSEPK 43 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2627 PE=4 SV=1
3 KRT84_HUMAN 34 Keratin, type II cuticular Hb4 OS=Homo sapiens GN=KRT84 PE=2 SV=2
2 Q88RE4_PSEPK 40 description

+13

Accession Score Description
1 Q88E50_PSEPK 43 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4618 PE=4 SV=1

-14

Accession Score Description
1 K1H1_HUMAN 42 Keratin, type I cuticular Ha1 OS=Homo sapiens GN=KRT31 PE=2 SV=3
Score Mass Matches Sequences emPAI
14.1 K1H1_HUMAN 42 48633 6 (1) 4 (1) 0.07
Keratin, type I cuticular Ha1 OS=Homo sapiens GN=KRT31 PE=2 SV=3
9 samesets of K1H1_HUMAN
K1M1_SHEEP 42 48070 4 (1) 4 (1) 0.07
Keratin, type I microfibrillar 48 kDa, component 8C-1 OS=Ovis aries PE=1 SV=2
KRT35_HUMAN 42 51640 3 (1) 3 (1) 0.06
Keratin, type I cuticular Ha5 OS=Homo sapiens GN=KRT35 PE=2 SV=5
K1C10_HUMAN 42 59020 2 (1) 2 (1) 0.06
Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6
KT33B_HUMAN 42 47325 5 (1) 3 (1) 0.07
Keratin, type I cuticular Ha3-II OS=Homo sapiens GN=KRT33B PE=2 SV=3
KRT36_HUMAN 42 53354 2 (1) 2 (1) 0.06
Keratin, type I cuticular Ha6 OS=Homo sapiens GN=KRT36 PE=1 SV=1
K1C15_SHEEP 42 48740 1 (1) 1 (1) 0.07
Keratin, type I cytoskeletal 15 OS=Ovis aries GN=KRT15 PE=2 SV=1
K1H2_HUMAN 42 51793 1 (1) 1 (1) 0.06
Keratin, type I cuticular Ha2 OS=Homo sapiens GN=KRT32 PE=1 SV=3
KRT37_HUMAN 42 51084 1 (1) 1 (1) 0.06
Keratin, type I cuticular Ha7 OS=Homo sapiens GN=KRT37 PE=2 SV=3
KRT38_HUMAN 42 52044 1 (1) 1 (1) 0.06
Keratin, type I cuticular Ha8 OS=Homo sapiens GN=KRT38 PE=2 SV=3

-6 peptide matches (5 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2550   404.2045 806.3944 806.3923 2.72 0 42 0.00058 +1Score > 23 indicates identity U K.LAADDFR.T
3055 +1 396.5445 1186.6117 1186.5829 24.2 0 3 6.5 +9Score > 23 indicates identity R.DNAELENLIR.E + Deamidated (NQ)
3057   594.3148 1186.6150 1186.5829 27.1 0 1 9.6 +9Score > 23 indicates identity R.DNAELENLIR.E + Deamidated (NQ)
3091   610.3200 1218.6254 1218.5979 22.6 0 4 5.3 +6Score > 23 indicates identity K.SDLEAQVESLK.E + Deamidated (NQ)
3129   415.5461 1243.6165 1243.6408 -19.6 0 2 5.9 +7Score > 23 indicates identity
Score > 22 indicates homology		 R.QLVESDINGLR.R + Deamidated (NQ)

+15

Accession Score Description
1 Q88PC1_PSEPK 42 Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_0929 PE=3 SV=1

+16

Accession Score Description
1 Q88JV3_PSEPK 40 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2544 PE=4 SV=1

+17

Accession Score Description
1 KRA3A_SHEEP 38 Keratin, high-sulfur matrix protein, IIIA3A OS=Ovis aries PE=1 SV=1

+18

Accession Score Description
1 Q88ND7_PSEPK 38 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1275 PE=4 SV=1

+19

Accession Score Description
1 Q88GM9_PSEPK 38 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_3692 PE=4 SV=1

+20

Accession Score Description
1 Q88D01_PSEPK 37 Proline iminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pip PE=3 SV=1
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