MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 105)

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+11

Accession Score Description
1 Q88KQ0_PSEPK 44 ABC efflux transporter, permease/ATP-binding protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_2240 PE=3 SV=1

-12

Accession Score Description
Family member distances as a dendrogram 1 Q88JM2_PSEPK 43 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2627 PE=4 SV=1
3 KRT84_HUMAN 34 Keratin, type II cuticular Hb4 OS=Homo sapiens GN=KRT84 PE=2 SV=2
2 Q88RE4_PSEPK 40 description
Cut threshold

Score Mass Matches Sequences emPAI
Q88JM2_PSEPK 43 135367 7 (2) 5 (2) 0.05
Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2627 PE=4 SV=1
Q88RE4_PSEPK 40 0 3 (3) 2 (2) 0.40
description
KRT84_HUMAN 34 65942 6 (2) 3 (2) 0.10
Keratin, type II cuticular Hb4 OS=Homo sapiens GN=KRT84 PE=2 SV=2

-14 peptide matches (12 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
2459 +2 704.3601 703.3528 703.3501 3.94 0 20 0.12 +1Score > 23 indicates identity U X R.DALTER.L
2472   716.4293 715.4220 715.4228 -1.13 0 29 0.013 +2Score > 23 indicates identity U X R.LLDTVR.D
2489   730.4106 729.4033 729.4021 1.72 0 23 0.058 +3Score > 23 indicates identity U X R.ELLAER.L
2490   365.7100 729.4054 729.4021 4.63 0 26 0.034 +3Score > 23 indicates identity U X R.ELLAER.L
2783   994.5327 993.5254 993.5356 -10.2 0 5 1.1 +2Score > 22 indicates identity
Score > 18 indicates homology		 U X K.AALQAVHQR.F + Deamidated (NQ)
2822   508.7600 1015.5054 1015.5046 0.80 1 11 0.94 +8Score > 23 indicates identity U X R.IDQAQARGR.Q + 2 Deamidated (NQ)
3411   748.3948 1494.7750 1494.7289 30.9 1 5 2.3 +2Score > 22 indicates identity
Score > 21 indicates homology		 U X K.WSFLQEQKCIR.S + Deamidated (NQ)
3412   374.7012 1494.7757 1494.7289 31.3 1 10 0.94 +2Score > 22 indicates identity U X K.WSFLQEQKCIR.S + Deamidated (NQ)
3413   499.2668 1494.7786 1494.7289 33.2 1 7 1.7 +2Score > 22 indicates identity U X K.WSFLQEQKCIR.S + Deamidated (NQ)
4045   588.7880 2351.1229 2351.0270 40.8 1 16 0.037 +1Score > 22 indicates identity
Score > 14 indicates homology		 U X K.YEEMQVTAGQHCDNLRNIR.N + Oxidation (M); 2 Deamidated (NQ)
4049   785.0449 2352.1129 2352.0110 43.3 1 2 1 +2Score > 22 indicates identity
Score > 14 indicates homology		 U X K.YEEMQVTAGQHCDNLRNIR.N + Oxidation (M); 3 Deamidated (NQ)

3 subsets and intersections (10 subset proteins in total)

Score Mass Subset of
Q877M4_PSEPK 38 0 12.1
description
7 samesets of Q877M4_PSEPK
Q88LB1_PSEPK 38 136352
Exonuclease SbcC OS=Pseudomonas putida (strain KT2440) GN=sbcC PE=4 SV=1
Q88PC6_PSEPK 38 0
description
Q88JT8_PSEPK 38 48643
Metalloprotease secretion protein OS=Pseudomonas putida (strain KT2440) GN=hasE PE=4 SV=1
Q88FL8_PSEPK 38 0
description
Q88FK2_PSEPK 38 0
description
Q88G97_PSEPK 38 0
description
Q88Q60_PSEPK 38 0
description
Q88I25_PSEPK 29 44163 12.1
Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_3177 PE=4 SV=1
Q88GP4_PSEPK 26 0 12.2
description

+13

Accession Score Description
1 Q88E50_PSEPK 43 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4618 PE=4 SV=1

+14

Accession Score Description
1 K1H1_HUMAN 42 Keratin, type I cuticular Ha1 OS=Homo sapiens GN=KRT31 PE=2 SV=3

+15

Accession Score Description
1 Q88PC1_PSEPK 42 Lipoprotein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_0929 PE=3 SV=1

+16

Accession Score Description
1 Q88JV3_PSEPK 40 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_2544 PE=4 SV=1

+17

Accession Score Description
1 KRA3A_SHEEP 38 Keratin, high-sulfur matrix protein, IIIA3A OS=Ovis aries PE=1 SV=1

+18

Accession Score Description
1 Q88ND7_PSEPK 38 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1275 PE=4 SV=1

+19

Accession Score Description
1 Q88GM9_PSEPK 38 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_3692 PE=4 SV=1

+20

Accession Score Description
1 Q88D01_PSEPK 37 Proline iminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pip PE=3 SV=1
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