MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 101–105 (out of 105)

Page: Previous 1 …6 7 8 9 10 11 

+101

Accession Score Description
1 Q88EZ5_PSEPK 16 Periplasmic thiosulfate-binding protein OS=Pseudomonas putida (strain KT2440) GN=PP_4305 PE=4 SV=1

+102

Accession Score Description
1 Q88PB2_PSEPK 15 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_0938 PE=4 SV=1

+103

Accession Score Description
1 Q88F76_PSEPK 15 SyrP protein, putative OS=Pseudomonas putida (strain KT2440) GN=PP_4222 PE=4 SV=1

-104

Accession Score Description
1 SYP_PSEPK 14 Proline--tRNA ligase OS=Pseudomonas putida (strain KT2440) GN=proS PE=3 SV=1
Score Mass Matches Sequences emPAI
104.1 SYP_PSEPK 14 63755 4 (1) 3 (1) 0.05
Proline--tRNA ligase OS=Pseudomonas putida (strain KT2440) GN=proS PE=3 SV=1
2 samesets of SYP_PSEPK
Q88FL7_PSEPK 14 54305 3 (1) 3 (1) 0.06
Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_4072 PE=4 SV=1
Q88N04_PSEPK 14 60507 1 (1) 1 (1) 0.05
5'-nucleotidase OS=Pseudomonas putida (strain KT2440) GN=ushA PE=3 SV=1

-4 peptide matches (3 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2190 +1 547.3010 546.2937 546.2948 -1.94 0 14 0.5 +6Score > 24 indicates identity U R.AGMIR.K
2540   399.2660 796.5174 796.5534 -45.2 0 14 0.043 +1Score > 13 indicates identity U K.LIALIVR.G
4386   589.5057 2942.4921 2942.3545 46.8 1 0 1 +1Score > 22 indicates identity
Score > 13 indicates homology		 U R.SVALMSDFGIGANIDDKHYFGVNWER.D + 2 Deamidated (NQ)

+105

Accession Score Description
1 Q88J48_PSEPK 14 Aldehyde dehydrogenase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_2801 PE=3 SV=1
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