MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2683a
MS data file : Andy2683a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:54:18 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,770

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 105)

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+1

Accession Score Description
1 3FLAG_TEV_PP_2683 7938 Fusion_protein_N_term_3FLAGTEV_in_frame_with_PP_2683

+2

Accession Score Description
1 Q88JG7_PSEPK 1226 Alcohol dehydrogenase, iron-containing OS=Pseudomonas putida (strain KT2440) GN=PP_2682 PE=4 SV=1

+3

Accession Score Description
1 TRYP_PIG 210 Trypsin OS=Sus scrofa PE=1 SV=1

+4

Accession Score Description
1 CH60_PSEPK 142 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1

-5

Accession Score Description
1 Q88M80_PSEPK 82 Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1694 PE=4 SV=1
Score Mass Matches Sequences emPAI
5.1 Q88M80_PSEPK 82 55707 18 (5) 7 (2) 0.19
Uncharacterized protein OS=Pseudomonas putida (strain KT2440) GN=PP_1694 PE=4 SV=1

-18 peptide matches (10 non-duplicate, 8 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2160 +1 529.3467 528.3394 528.3384 2.01 0 29 0.011 +1Score > 22 indicates identity U K.LLGAR.A
2278   617.3265 616.3192 616.3180 1.97 0 23 0.082 +10Score > 24 indicates identity U R.EIAER.A
2321   634.2952 633.2879 633.2904 -3.97 0 3 3.2 +10Score > 20 indicates identity U K.LCEGR.G
2488   730.3755 729.3682 729.4021 -46.4 0 3 1 +1Score > 22 indicates identity
Score > 16 indicates homology		 U R.LQQVDK.A
2566 +1 815.5359 814.5286 814.5025 32.1 1 7 0.59 +5Score > 17 indicates identity U K.LLGARASK.S
2569 -3 408.2718 814.5290 814.5025 32.6 1 12 0.17 +4Score > 17 indicates identity U K.LLGARASK.S
2565   408.2706 814.5266 814.5025 29.7 1 (12) 0.17 +4Score > 17 indicates identity U K.LLGARASK.S
2567   408.2717 814.5288 814.5025 32.4 1 (12) 0.17 +4Score > 17 indicates identity U K.LLGARASK.S
2570   408.2723 814.5300 814.5025 33.9 1 (10) 0.25 +5Score > 17 indicates identity U K.LLGARASK.S
2644   444.2383 886.4620 886.4760 -15.7 0 7 2 +8Score > 23 indicates identity U R.LQQLNLR.L + 3 Deamidated (NQ)
2744 +1 487.2520 972.4894 972.4876 1.91 0 21 0.095 +2Score > 23 indicates identity U R.LEDLQEAR.D
2746   974.4813 973.4740 973.4716 2.49 0 37 0.0022 +2Score > 23 indicates identity U R.LEDLQEAR.D + Deamidated (NQ)
2747 +2 487.7444 973.4742 973.4716 2.72 0 47 0.00024 +2Score > 23 indicates identity U R.LEDLQEAR.D + Deamidated (NQ)

1 subset or intersection (2 subset proteins in total)

Score Mass Subset of
Q88QJ4_PSEPK 29 0 5.1
description
1 sameset of Q88QJ4_PSEPK
SYL_PSEPK 29 97207
Leucine--tRNA ligase OS=Pseudomonas putida (strain KT2440) GN=leuS PE=3 SV=1

+6

Accession Score Description
1 RS4_PSEPK 69 30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1

+7

Accession Score Description
1 Q88I44_PSEPK 60 Inositol monophosphatase family protein OS=Pseudomonas putida (strain KT2440) GN=PP_3157 PE=4 SV=1

+8

Accession Score Description
1 Q88IY0_PSEPK 50 Oxidoreductase, FMN-binding OS=Pseudomonas putida (strain KT2440) GN=PP_2869 PE=4 SV=1

+9

Accession Score Description
Family member distances as a dendrogram 1 Q88JT1_PSEPK 46 Leucine-rich repeat domain protein OS=Pseudomonas putida (strain KT2440) GN=PP_2566 PE=4 SV=1
2 Q88BY3_PSEPK 38 Transposition protein, TnsC-related protein OS=Pseudomonas putida (strain KT2440) GN=PP_5404 PE=4 SV=1

+10

Accession Score Description
1 Q88L42_PSEPK 44 Response regulator NasT OS=Pseudomonas putida (strain KT2440) GN=nasT PE=4 SV=1
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