MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4201–4300 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4488 1112.9917 5559.9221
4489 1113.1871 5560.8991
4490 927.8265 5560.9153
4491 927.8274 5560.9207
4492 930.3273 5575.9201
4493 1116.5854 5577.8906
4494 937.3299 5617.9357
4495 1124.6007 5617.9671
4496 1134.4043 5666.9851
4497 1146.3792 5726.8596
4498 1146.3922 5726.9246
4499 1149.3823 5741.8751
4500 1151.7917 5753.9221
4501 1153.1841 5760.8841
4503 1155.9802 5774.8646
4504 1158.5886 5787.9066
4505 1159.1825 5790.8761
4506 1161.5878 5802.9026
4507 1162.5834 5807.8806
4508 1162.7870 5808.8986
4509 969.1580 5808.9043
4510 1164.3954 5816.9406
4511 1164.5863 5817.8951
4512 832.1379 5817.9144
4513 970.6682 5817.9655
4514 1165.5865 5822.8961
4515 1165.9872 5824.8996
4516 972.9888 5831.8891
4517 1167.7902 5833.9146
4518 835.1357 5838.8990
4519 974.3243 5839.9021
4520 1170.3861 5846.8941
4521 975.6542 5847.8815
4522 1170.8043 5848.9851
4524 1171.7827 5853.8771
4525 976.8176 5854.8619
4526 837.5581 5855.8558
4527 977.3360 5857.9723
4528 1173.7999 5863.9631
4529 1175.1777 5870.8521
4530 839.9833 5872.8322
4531 979.8209 5872.8817
4532 840.1420 5873.9431
4533 1175.9944 5874.9356
4534 980.9754 5879.8087
4535 981.4775 5882.8213
4536 982.1539 5886.8797
4537 982.8127 5890.8325
4538 1179.3861 5891.8941
4539 983.6530 5895.8743
4540 984.9776 5903.8219
4541 985.3221 5905.8889
4542 1182.5811 5907.8691
4543 1183.1697 5910.8121
4544 1184.9728 5919.8276
4545 987.8403 5920.9981
4547 990.3395 5935.9933
4548 990.5076 5937.0019
4549 1188.6106 5938.0166
4550 992.0118 5946.0271
4551 994.6804 5962.0387
4553 995.5216 5967.0859
4554 995.9974 5969.9407
4555 854.2934 5973.0029
4557 998.3341 5983.9609
4558 1000.3374 5995.9807
4560 1000.5140 5997.0403
4564 1003.0183 6012.0661
4571 1005.6713 6027.9841
4574 1008.1758 6043.0111
4575 1008.1766 6043.0159
4576 1008.1772 6043.0195
4577 1008.3557 6044.0905
4578 1009.1714 6048.9847
4579 865.2897 6049.9770
4580 1010.3489 6056.0497
4581 866.2830 6056.9301
4582 1010.5096 6057.0139
4583 1010.5117 6057.0265
4584 1010.5119 6057.0277
4585 1011.1723 6060.9901
4586 867.4310 6064.9661
4587 867.4376 6065.0123
4589 1011.9986 6065.9479
4590 1012.9930 6071.9143
4591 1013.0054 6071.9887
4592 1013.3295 6073.9333
4595 869.7108 6080.9247
4596 1015.0106 6084.0199
4597 1015.0198 6084.0751
4598 1015.1805 6085.0393
4600 1016.1639 6090.9397
4601 1017.3332 6097.9555
4602 1017.3455 6098.0293
4603 1017.5148 6099.0451
4605 1018.1568 6102.8971
4606 873.1360 6104.9011
4607 1018.4985 6104.9473
4608 1019.5048 6110.9851
4609 1019.6763 6112.0141
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