MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4225 846.4210 3381.6549
4227 847.4180 3385.6429
4228 847.6569 3386.5985
4229 678.3279 3386.6031
4230 847.9279 3387.6825
4231 1130.8978 3389.6716
4232 848.6658 3390.6341
4235 1131.5930 3391.7572
4236 849.1635 3392.6249
4237 1131.8865 3392.6377
4238 849.4129 3393.6225
4239 849.4241 3393.6673
4240 1132.2548 3393.7426
4241 849.4465 3393.7569
4242 1133.9104 3398.7094
4243 852.4282 3405.6837
4244 852.8966 3407.5573
4245 1138.2157 3411.6253
4246 1140.2611 3417.7615
4247 855.6945 3418.7489
4248 858.9267 3431.6777
4249 860.4386 3437.7253
4250 861.9252 3443.6717
4251 863.4449 3449.7505
4252 1151.2578 3450.7516
4253 868.4474 3469.7605
4254 1157.5950 3469.7632
4258 1172.5370 3514.5892
4262 1174.9501 3521.8285
4263 881.7152 3522.8317
4264 1175.2888 3522.8446
4268 1188.6357 3562.8853
4269 1194.5835 3580.7287
4270 899.6951 3594.7513
4272 918.2119 3668.8185
4273 918.2213 3668.8561
4274 735.9791 3674.8591
4277 922.9797 3687.8897
4278 739.5728 3692.8276
4279 742.1638 3705.7826
4281 928.9809 3711.8945
4282 934.7323 3734.9001
4285 938.7125 3750.8209
4286 943.4301 3769.6913
4288 756.1349 3775.6381
4289 630.2820 3775.6483
4291 630.2833 3775.6561
4293 756.1436 3775.6816
4294 630.4459 3776.6317
4295 756.3372 3776.6496
4296 756.3380 3776.6536
4297 630.4496 3776.6539
4298 756.3384 3776.6556
4299 756.3386 3776.6566
4300 630.4501 3776.6569
4301 756.3398 3776.6626
4302 756.3398 3776.6626
4303 756.3404 3776.6656
4304 630.4521 3776.6689
4305 630.4542 3776.6815
4306 756.3438 3776.6826
4308 946.4908 3781.9341
4309 757.3957 3781.9421
4310 946.7364 3782.9165
4311 946.7366 3782.9173
4312 759.3339 3791.6331
4313 759.5369 3792.6481
4314 950.4836 3797.9053
4315 950.4878 3797.9221
4316 950.4924 3797.9405
4318 762.7343 3808.6351
4319 762.9383 3809.6551
4320 953.4860 3809.9149
4322 955.2486 3816.9653
4323 764.9835 3819.8811
4324 765.7314 3823.6206
4325 766.1379 3825.6531
4326 958.5312 3830.0957
4328 960.4968 3837.9581
4329 961.4634 3841.8245
4330 966.7154 3862.8325
4333 777.1828 3880.8776
4334 651.6323 3903.7501
4335 651.7962 3904.7335
4336 651.7990 3904.7503
4337 651.8009 3904.7617
4338 784.8041 3918.9841
4339 980.7543 3918.9881
4341 787.8002 3933.9646
4342 984.5005 3933.9729
4343 986.9954 3943.9525
4344 791.7463 3953.6951
4345 659.9579 3953.7037
4346 659.9583 3953.7061
4347 791.7491 3953.7091
4348 989.5013 3953.9761
4349 791.9492 3954.7096
4350 792.8267 3959.0971
4351 997.0163 3984.0361
4352 802.7990 4008.9586
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