MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4088 761.1453 3040.5521
4089 1019.9033 3056.6881
4091 613.2964 3061.4456
4092 1024.2286 3069.6640
4093 1024.8818 3071.6236
4094 1025.5763 3073.7071
4097 769.6852 3074.7117
4099 1028.5620 3082.6642
4101 1032.5804 3094.7194
4102 1033.5052 3097.4938
4103 1035.8901 3104.6485
4106 1042.0646 3123.1720
4107 1044.5294 3130.5664
4108 783.9416 3131.7373
4111 784.9026 3135.5813
4112 1046.8615 3137.5627
4113 785.4387 3137.7257
4115 629.3293 3141.6101
4116 1050.2382 3147.6928
4117 1050.8628 3149.5666
4118 1052.8546 3155.5420
4119 1052.9076 3155.7010
4120 1057.5291 3169.5655
4121 635.7669 3173.7981
4122 794.4576 3173.8013
4123 1061.9238 3182.7496
4125 1066.5396 3196.5970
4126 804.4036 3213.5853
4127 647.1719 3230.8231
4128 647.8027 3233.9771
4129 1079.8693 3236.5861
4131 649.5410 3242.6686
4132 811.6746 3242.6693
4133 1085.9309 3254.7709
4134 816.1970 3260.7589
4135 1088.9255 3263.7547
4136 1089.2020 3264.5842
4139 818.1608 3268.6141
4140 1090.5553 3268.6441
4141 818.1695 3268.6489
4157 1094.2820 3279.8242
4158 822.1678 3284.6421
4159 823.6650 3290.6309
4160 1099.2562 3294.7468
4161 1099.2650 3294.7732
4162 1099.2676 3294.7810
4163 824.7048 3294.7901
4164 1099.5957 3295.7653
4165 1101.2604 3300.7594
4166 1101.2614 3300.7624
4167 1101.5891 3301.7455
4168 826.4472 3301.7597
4169 1101.5973 3301.7701
4170 826.4532 3301.7837
4171 661.7533 3303.7301
4172 1103.2657 3306.7753
4173 827.7015 3306.7769
4174 662.5269 3307.5981
4175 1103.5487 3307.6243
4176 1106.0715 3315.1927
4177 664.3479 3316.7031
4178 830.1854 3316.7125
4179 1106.5853 3316.7341
4180 830.1910 3316.7349
4181 830.1932 3316.7437
4182 830.1938 3316.7461
4183 830.1938 3316.7461
4184 1106.5905 3316.7497
4185 1106.6013 3316.7821
4186 1107.3145 3318.9217
4187 1107.9392 3320.7958
4188 831.6940 3322.7469
4190 832.6746 3326.6693
4191 1110.5947 3328.7623
4192 833.6954 3330.7525
4193 833.9387 3331.7257
4194 834.1786 3332.6853
4195 1111.9070 3332.6992
4196 834.1835 3332.7049
4197 834.4106 3333.6133
4199 667.7444 3333.6856
4201 834.6606 3334.6133
4203 835.6956 3338.7533
4204 835.6992 3338.7677
4205 1114.2316 3339.6730
4207 835.9368 3339.7181
4208 836.6777 3342.6817
4209 837.1698 3344.6501
4211 1116.2432 3345.7078
4212 838.1848 3348.7101
4213 671.9405 3354.6661
4214 1119.5626 3355.6660
4215 839.9282 3355.6837
4216 841.1843 3360.7081
4218 841.9243 3363.6681
4219 1124.5503 3370.6291
4220 843.6652 3370.6317
4222 843.9224 3371.6605
4223 675.3425 3371.6761
4224 1127.5778 3379.7116
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