| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy2682a |
| MS data file | : | Andy2682a.mgf |
| Database | : | P-putida 20180924 (5,556 sequences; 1,892,173 residues) |
| Timestamp | : | 2 Aug 2026 at 00:52:38 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 50 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | Default |
| Number of queries | : | 4,656 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 720.8580 | 1439.7014 | 1439.7191 | -12.2 | 1 | 6 | 1 | 1Score > 22 indicates identityScore > 19 indicates homology |
NSRVMLLASYNR + Oxidation (M); Deamidated (NQ) | |
| 572.2969 | 1142.5792 | 1142.6230 | -38.3 | 1 | 6 | 0.57 | 1Score > 22 indicates identityScore > 16 indicates homology |
RMTIAVPAER | |
| 650.8210 | 1299.6274 | 1299.6717 | -34.1 | 1 | 6 | 0.56 | 1Score > 22 indicates identityScore > 16 indicates homology |
IADGKLNRPCR + Deamidated (NQ) | |
| 604.3815 | 1206.7484 | 1206.7197 | 23.9 | 1 | 6 | 0.26 | 1Score > 13 indicates identity |
LVHTLARQLR + Deamidated (NQ) | |
| 680.3486 | 1358.6826 | 1358.7293 | -34.3 | 0 | 6 | 0.82 | 1Score > 23 indicates identityScore > 17 indicates homology |
ITTLIENGELEK | |
| 512.8005 | 1023.5864 | 1023.5964 | -9.73 | 1 | 6 | 0.82 | 1Score > 17 indicates identity |
LAISNPKALP + Deamidated (NQ) | |
| 450.9045 | 1349.6917 | 1349.7527 | -45.2 | 1 | 6 | 0.37 | 1Score > 22 indicates identityScore > 14 indicates homology |
SRNALGNLGHALK | |
| 478.2508 | 1431.7306 | 1431.6895 | 28.7 | 1 | 6 | 0.75 | 1Score > 23 indicates identityScore > 17 indicates homology |
TYPPQAWERQR + Deamidated (NQ) | |
| 579.3032 | 1156.5918 | 1156.6353 | -37.6 | 1 | 6 | 0.55 | 1Score > 23 indicates identityScore > 16 indicates homology |
FVRLHAETGK | |
| 543.7562 | 2170.9957 | 2171.0494 | -24.8 | 1 | 6 | 0.37 | 1Score > 22 indicates identityScore > 14 indicates homology |
AETGINYENRSLGTTQLFR + 2 Deamidated (NQ) | |
| 1185.8623 | 4739.4201 | 4739.3615 | 12.4 | 0 | 6 | 1.1 | 1Score > 18 indicates identity |
LQGQVFDLIFVNAGVMGPLPQDLEAVHNSDIGDLFMTNAVAPIR + Oxidation (M); 2 Deamidated (NQ) | |
| 531.2810 | 1060.5474 | 1060.5149 | 30.7 | 0 | 6 | 1 | 1Score > 23 indicates identityScore > 18 indicates homology |
INGSGSLQQR + 2 Deamidated (NQ) | |
| 436.7413 | 871.4680 | 871.4664 | 1.87 | 1 | 6 | 0.8 | 1Score > 23 indicates identityScore > 17 indicates homology |
FNHAKVR + Deamidated (NQ) | |
| 491.2784 | 980.5422 | 980.5654 | -23.7 | 1 | 6 | 1.1 | 1Score > 19 indicates identity |
ELEKLPPR | |
| 313.5043 | 937.4911 | 937.4617 | 31.3 | 1 | 6 | 0.54 | 1Score > 20 indicates identityScore > 15 indicates homology |
RSADQYAK | |
| 509.9312 | 1526.7718 | 1526.8192 | -31.0 | 0 | 6 | 0.43 | 1Score > 23 indicates identityScore > 14 indicates homology |
QAGVAIEQVSVPSIK + 2 Deamidated (NQ) | |
| 433.2560 | 864.4974 | 864.5042 | -7.81 | 1 | 6 | 0.71 | 1Score > 17 indicates identity |
LGRNHLR | |
| 605.8066 | 1209.5986 | 1209.6063 | -6.33 | 1 | 6 | 0.33 | 1Score > 21 indicates identityScore > 13 indicates homology |
NNLLNFKMAK + Oxidation (M); 2 Deamidated (NQ) | |
| 1059.5486 | 2117.0826 | 2117.0609 | 10.3 | 1 | 6 | 0.31 | 1Score > 23 indicates identityScore > 13 indicates homology |
GVVMLERDVEQATLAEMAR | |
| 328.5127 | 982.5163 | 982.4906 | 26.1 | 1 | 6 | 0.5 | 1Score > 21 indicates identityScore > 15 indicates homology |
QKVGTCYK | |
| 615.3568 | 1228.6990 | 1228.6411 | 47.2 | 1 | 5 | 0.84 | 1Score > 21 indicates identityScore > 17 indicates homology |
RNLLEQIQGR + 3 Deamidated (NQ) | |
| 713.3563 | 2137.0471 | 2137.1354 | -41.3 | 1 | 5 | 0.5 | 1Score > 23 indicates identityScore > 15 indicates homology |
FMGVAAERIAEGAFLLALSR + Oxidation (M) | |
| 350.5461 | 1048.6165 | 1048.6505 | -32.5 | 1 | 5 | 0.97 | 1Score > 18 indicates identity |
VARLVPAPAR | |
| 450.2217 | 898.4288 | 898.4443 | -17.2 | 1 | 5 | 1 | 1Score > 18 indicates identity |
MRGHQAAK + Deamidated (NQ) | |
| 412.2435 | 1233.7087 | 1233.6717 | 30.0 | 1 | 5 | 1.1 | 1Score > 18 indicates identity |
IKAAGVDYELR | |
| 725.8655 | 1449.7164 | 1449.6736 | 29.6 | 0 | 5 | 1 | 1Score > 23 indicates identityScore > 18 indicates homology |
ELVLFDDQSNNR + Deamidated (NQ) | |
| 810.9464 | 3239.7565 | 3239.7336 | 7.08 | 1 | 5 | 1.3 | 1Score > 19 indicates identity |
RQAKPEHKPSLEELLEMLVEQALAVQPR + Deamidated (NQ) | |
| 470.7395 | 939.4644 | 939.4662 | -1.82 | 1 | 5 | 0.85 | 1Score > 20 indicates identityScore > 17 indicates homology |
FSSQTKDK | |
| 800.3928 | 2398.1566 | 2398.2315 | -31.2 | 1 | 5 | 1 | 1Score > 22 indicates identityScore > 18 indicates homology |
QMQSNLGQKTWAPSAVPLELGK + Oxidation (M) | |
| 393.2300 | 784.4454 | 784.4807 | -44.9 | 0 | 5 | 1 | 1Score > 20 indicates identityScore > 18 indicates homology |
LLSPSLR | |
| 1192.1017 | 2382.1888 | 2382.1452 | 18.3 | 0 | 5 | 1 | 1Score > 23 indicates identityScore > 18 indicates homology |
APDDLLDHLENTIGGAGADYVAR | |
| 730.4235 | 1458.8324 | 1458.7653 | 46.0 | 0 | 5 | 1 | 1Score > 20 indicates identityScore > 18 indicates homology |
MANLGLANLIAWR + Oxidation (M); Deamidated (NQ) | |
| 694.3525 | 1386.6904 | 1386.7103 | -14.3 | 1 | 5 | 0.91 | 1Score > 23 indicates identityScore > 17 indicates homology |
ERAALVLESEDR | |
| 415.8985 | 1244.6737 | 1244.6625 | 8.95 | 1 | 5 | 1 | 1Score > 22 indicates identityScore > 18 indicates homology |
RSSWLEQALR | |
| 895.4396 | 2683.2970 | 2683.2356 | 22.9 | 1 | 5 | 0.72 | 1Score > 23 indicates identityScore > 16 indicates homology |
QRLQQNITQMDNMIGQVLDYLR + 7 Deamidated (NQ) | |
| 402.6898 | 1606.7301 | 1606.6722 | 36.0 | 0 | 5 | 1 | 1Score > 21 indicates identityScore > 18 indicates homology |
AFSSMENFTNWTR + Oxidation (M); Deamidated (NQ) | |
| 761.9093 | 1521.8040 | 1521.7688 | 23.2 | 1 | 5 | 0.85 | 1Score > 22 indicates identityScore > 17 indicates homology |
IAGFARGYSAQPER | |
| 407.7617 | 813.5088 | 813.5072 | 2.00 | 0 | 5 | 0.96 | 1Score > 22 indicates identityScore > 18 indicates homology |
LATLLQR | |
| 363.8639 | 1088.5699 | 1088.5614 | 7.74 | 1 | 5 | 1 | 1Score > 23 indicates identityScore > 18 indicates homology |
PTPRQFSQK + Deamidated (NQ) | |
| 802.3994 | 2404.1764 | 2404.1118 | 26.9 | 0 | 5 | 0.7 | 1Score > 22 indicates identityScore > 16 indicates homology |
YTDNFGKPHTLTEDQMVHVR + Oxidation (M); Deamidated (NQ) | |
| 585.9496 | 1754.8270 | 1754.8111 | 9.04 | 1 | 5 | 0.96 | 1Score > 21 indicates identityScore > 17 indicates homology |
ADATLAYAWGKNSSDGK + Deamidated (NQ) | |
| 918.8046 | 2753.3920 | 2753.4025 | -3.81 | 1 | 5 | 1 | 1Score > 22 indicates identityScore > 18 indicates homology |
LNVVIGDEDPLGSFVFRTTGFNSIR + Deamidated (NQ) | |
| 566.2484 | 1130.4822 | 1130.5067 | -21.6 | 0 | 5 | 0.83 | 1Score > 17 indicates identity |
ITFEGDFMR + Oxidation (M) | |
| 377.4186 | 1505.6453 | 1505.6456 | -0.22 | 0 | 5 | 1.1 | 1Score > 18 indicates identity |
MADWQSLDPEAAR + Oxidation (M); Deamidated (NQ) | |
| 880.9843 | 1759.9540 | 1759.9468 | 4.11 | 1 | 5 | 0.77 | 1Score > 21 indicates identityScore > 16 indicates homology |
ALRSVQTLFEELTPR + Deamidated (NQ) | |
| 1060.0438 | 2118.0730 | 2118.1031 | -14.2 | 0 | 5 | 0.43 | 1Score > 23 indicates identityScore > 14 indicates homology |
IFQEASPAISGVIQGAMILR + Oxidation (M); 2 Deamidated (NQ) | |
| 634.8193 | 1267.6240 | 1267.6448 | -16.4 | 0 | 5 | 1 | 1Score > 21 indicates identityScore > 18 indicates homology |
VPSQYYLELR + Deamidated (NQ) | |
| 1155.1138 | 2308.2130 | 2308.1429 | 30.4 | 1 | 5 | 0.42 | 1Score > 22 indicates identityScore > 14 indicates homology |
MPARCRPWSRPAAGVPGTDAR | |
| 404.1928 | 1209.5566 | 1209.5513 | 4.35 | 0 | 5 | 0.75 | 1Score > 21 indicates identityScore > 16 indicates homology |
GITYNQEDLR + 2 Deamidated (NQ) | |
| 656.4133 | 1966.2181 | 1966.1211 | 49.3 | 1 | 5 | 0.32 | 1Score > 13 indicates identity |
LREALATVNGELQALVLR + Deamidated (NQ) | |
| 409.8906 | 1226.6500 | 1226.6870 | -30.2 | 0 | 5 | 0.76 | 1Score > 23 indicates identityScore > 16 indicates homology |
DPASVQLLAVSK | |
| 720.3905 | 2158.1497 | 2158.1019 | 22.2 | 0 | 5 | 1 | 1Score > 21 indicates identityScore > 17 indicates homology |
VFVVNADAIGGIGSSVANSGPAR + Deamidated (NQ) | |
| 929.4721 | 1856.9296 | 1856.8938 | 19.3 | 0 | 5 | 0.65 | 1Score > 23 indicates identityScore > 16 indicates homology |
TVNALEIELQAQHSMR + Oxidation (M); 2 Deamidated (NQ) | |
| 563.0315 | 2248.0969 | 2248.1964 | -44.3 | 1 | 5 | 0.44 | 1Score > 23 indicates identityScore > 14 indicates homology |
LGLRYDIRPDLQVYGNLSR + Deamidated (NQ) | |
| 873.4637 | 2617.3693 | 2617.4223 | -20.3 | 0 | 5 | 1 | 1Score > 21 indicates identityScore > 17 indicates homology |
LAQTPSVQPVLMDIEMPLVPVLAR + Deamidated (NQ) | |
| 511.7936 | 1021.5726 | 1021.6033 | -30.0 | 0 | 5 | 1.5 | 1Score > 19 indicates identity |
LAQSVVLHR | |
| 696.1851 | 3475.8891 | 3475.8021 | 25.0 | 0 | 5 | 1.3 | 1Score > 18 indicates identity |
TAQIAEAAGIALYGGTMLEGSIGTLASAHAFLTLR + Deamidated (NQ) | |
| 518.2757 | 1551.8053 | 1551.7524 | 34.1 | 0 | 5 | 1 | 1Score > 22 indicates identityScore > 17 indicates homology |
LMPVMAVSNSTLQK + 2 Oxidation (M); 2 Deamidated (NQ) | |
| 579.7950 | 1157.5754 | 1157.5605 | 13.0 | 0 | 5 | 1 | 1Score > 23 indicates identityScore > 17 indicates homology |
FSVAQTETFK + Deamidated (NQ) | |
| 864.7540 | 2591.2402 | 2591.3278 | -33.8 | 1 | 5 | 0.4 | 1Score > 22 indicates identityScore > 13 indicates homology |
VVAGDQAAGAQLQEMLRPFVYRR + Oxidation (M); Deamidated (NQ) | |
| 929.5356 | 928.5283 | 928.5025 | 27.8 | 1 | 5 | 1 | 1Score > 23 indicates identityScore > 17 indicates homology |
RMRPTPR + Oxidation (M) | |
| 474.2385 | 1419.6937 | 1419.7279 | -24.1 | 1 | 5 | 0.51 | 1Score > 23 indicates identityScore > 14 indicates homology |
IDTMTTSPADLKK | |
| 303.1599 | 604.3052 | 604.3003 | 8.25 | 0 | 5 | 0.75 | 1Score > 22 indicates identityScore > 16 indicates homology |
LEGMR | |
| 433.2208 | 432.2135 | 432.2332 | -45.6 | 0 | 5 | 0.59 | 1Score > 23 indicates identityScore > 15 indicates homology |
GATGK | |
| 759.4652 | 758.4579 | 758.4286 | 38.6 | 1 | 5 | 1 | 1Score > 22 indicates identityScore > 17 indicates homology |
KVQQQK + Deamidated (NQ) | |
| 552.8275 | 1103.6404 | 1103.5975 | 38.9 | 0 | 5 | 1.6 | 1Score > 19 indicates identity |
FVVAEPSTVR | |
| 344.1875 | 1029.5407 | 1029.4992 | 40.3 | 1 | 5 | 1 | 1Score > 23 indicates identityScore > 17 indicates homology |
RAEHENFK | |
| 623.3426 | 1244.6706 | 1244.6448 | 20.8 | 1 | 5 | 0.41 | 1Score > 22 indicates identityScore > 13 indicates homology |
ARLLMQWGDR | |
| 508.6099 | 1522.8079 | 1522.8355 | -18.1 | 0 | 5 | 0.45 | 1Score > 21 indicates identityScore > 14 indicates homology |
QAPVVANNVLVALGR + 3 Deamidated (NQ) | |
| 801.7528 | 2402.2366 | 2402.1965 | 16.7 | 0 | 5 | 1 | 1Score > 22 indicates identityScore > 17 indicates homology |
IGDSDNHVQLLEPLSPESPVQK + Deamidated (NQ) | |
| 714.3895 | 2140.1467 | 2140.1099 | 17.2 | 1 | 5 | 0.52 | 1Score > 22 indicates identityScore > 14 indicates homology |
LINGVVDPLSGAHMLERYR + Deamidated (NQ) | |
| 403.2516 | 804.4886 | 804.4857 | 3.60 | 0 | 5 | 1 | 1Score > 17 indicates identity |
LIYQIR | |
| 502.8891 | 1505.6455 | 1505.6529 | -4.91 | 1 | 5 | 1.2 | 1Score > 18 indicates identity |
AGRDQTDVAAEGCR + Deamidated (NQ) | |
| 546.6234 | 1636.8484 | 1636.8072 | 25.2 | 0 | 5 | 0.91 | 1Score > 22 indicates identityScore > 17 indicates homology |
ADMLHFPLEFFVR + Oxidation (M) | |
| 674.3920 | 2020.1542 | 2020.1429 | 5.59 | 1 | 5 | 1 | 1Score > 17 indicates identity |
VNVNIIGLDGRPQLKNLR + 2 Deamidated (NQ) | |
| 495.6025 | 1483.7857 | 1483.7743 | 7.67 | 1 | 5 | 0.53 | 1Score > 22 indicates identityScore > 14 indicates homology |
LAADGSPLSGGQKQR | |
| 673.3572 | 672.3499 | 672.3483 | 2.46 | 0 | 5 | 0.52 | 1Score > 22 indicates identityScore > 14 indicates homology |
ELPFAP | |
| 1050.5679 | 2099.1212 | 2099.0535 | 32.3 | 1 | 5 | 0.39 | 1Score > 21 indicates identityScore > 13 indicates homology |
ILLDGQPVTDFDPDQLRR + 2 Deamidated (NQ) | |
| 786.7573 | 2357.2501 | 2357.1711 | 33.5 | 0 | 5 | 0.74 | 1Score > 22 indicates identityScore > 16 indicates homology |
EQLDQVGSGGVTGATGLLGNNLVR + 3 Deamidated (NQ) | |
| 1055.5538 | 4218.1861 | 4218.0038 | 43.2 | 1 | 5 | 1.6 | 1Score > 19 indicates identity |
AIAPNAVNVNFYGTTETPQAMAFHTLDPEMDNARVPLGK + Oxidation (M); 2 Deamidated (NQ) | |
| 695.6404 | 2083.8994 | 2083.9741 | -35.8 | 0 | 4 | 1.4 | 1Score > 18 indicates identity |
MVFNGGALIGCDLGTMNVAK + Oxidation (M); Deamidated (NQ) | |
| 469.7269 | 937.4392 | 937.4559 | -17.7 | 0 | 4 | 0.9 | 1Score > 20 indicates identityScore > 17 indicates homology |
FAHFNFR | |
| 342.4850 | 1024.4332 | 1024.4317 | 1.39 | 0 | 4 | 1 | 1Score > 17 indicates identity |
MQESVMQR + Oxidation (M); Deamidated (NQ) | |
| 661.8522 | 1321.6898 | 1321.7103 | -15.4 | 0 | 4 | 0.75 | 1Score > 22 indicates identityScore > 16 indicates homology |
ATGELHVAGVGVGR | |
| 1064.5342 | 2127.0538 | 2127.0419 | 5.63 | 0 | 4 | 0.55 | 1Score > 23 indicates identityScore > 14 indicates homology |
AQIPHSIPTQTQGLFMNSR + 2 Deamidated (NQ) | |
| 1150.5631 | 1149.5558 | 1149.5124 | 37.8 | 0 | 4 | 0.88 | 1Score > 22 indicates identityScore > 16 indicates homology |
LWEMAENTR + Deamidated (NQ) | |
| 508.2764 | 1521.8074 | 1521.7973 | 6.61 | 0 | 4 | 0.68 | 1Score > 22 indicates identityScore > 15 indicates homology |
ALANTLACASVYLR | |
| 552.2673 | 551.2600 | 551.2591 | 1.66 | 0 | 4 | 1.2 | 1Score > 18 indicates identity |
AQYGI + Deamidated (NQ) | |
| 1080.5059 | 2158.9972 | 2159.1018 | -48.4 | 1 | 4 | 0.44 | 1Score > 21 indicates identityScore > 13 indicates homology |
AFMARGGQLQNNRPVTDIR + Oxidation (M) | |
| 479.6424 | 2393.1756 | 2393.1910 | -6.43 | 1 | 4 | 0.59 | 1Score > 22 indicates identityScore > 15 indicates homology |
HAGVFSVASEVQPGAQHVAAMRK + Oxidation (M); Deamidated (NQ) | |
| 702.8976 | 1403.7806 | 1403.7409 | 28.3 | 0 | 4 | 0.55 | 1Score > 20 indicates identityScore > 14 indicates homology |
FADGVATLLDAGVR | |
| 1042.9579 | 2083.9012 | 2083.9741 | -34.9 | 0 | 4 | 1.5 | 1Score > 19 indicates identity |
MVFNGGALIGCDLGTMNVAK + Oxidation (M); Deamidated (NQ) | |
| 955.4936 | 2863.4590 | 2863.5014 | -14.8 | 1 | 4 | 1 | 1Score > 22 indicates identityScore > 17 indicates homology |
LPSPHLLKQQMPLPSELAQQVQAHR + Oxidation (M); 2 Deamidated (NQ) | |
| 454.2441 | 1359.7105 | 1359.7034 | 5.20 | 1 | 4 | 1 | 1Score > 23 indicates identityScore > 17 indicates homology |
VEENPQLQKFK + Deamidated (NQ) | |
| 607.2546 | 606.2473 | 606.2544 | -11.7 | 0 | 4 | 0.77 | 1Score > 16 indicates identity |
AGSCGR | |
| 633.8958 | 1265.7770 | 1265.7278 | 38.9 | 1 | 4 | 0.51 | 1Score > 14 indicates identity |
ACLPLLRAQPK | |
| 425.8691 | 1274.5855 | 1274.5826 | 2.23 | 0 | 4 | 1 | 1Score > 21 indicates identityScore > 17 indicates homology |
NCVTAVGFDHR | |
| 822.7805 | 2465.3197 | 2465.3770 | -23.2 | 0 | 4 | 1 | 1Score > 21 indicates identityScore > 17 indicates homology |
IWQIVWAVAGMVGLVAGLLWGAR | |
| 703.0349 | 2106.0829 | 2106.0229 | 28.5 | 1 | 4 | 0.41 | 1Score > 23 indicates identityScore > 13 indicates homology |
GVTRQAGNEIDEFVTEALR + 2 Deamidated (NQ) | |
| 871.4581 | 4352.2541 | 4352.1039 | 34.5 | 0 | 4 | 1.7 | 1Score > 19 indicates identity |
QAAAAPSTPVEQQGGQPLAGLQVLCVDNEDSILIGMNSLLSR + 5 Deamidated (NQ) |
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