MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3947 874.7653 2621.2741
3950 658.0388 2628.1261
3952 877.3812 2629.1218
3955 878.0978 2631.2716
3956 658.8253 2631.2721
3957 878.1286 2631.3640
3958 659.0974 2632.3605
3959 879.4464 2635.3174
3960 660.0206 2636.0533
3961 880.0247 2637.0523
3962 881.4594 2641.3564
3963 881.7537 2642.2393
3964 661.5801 2642.2913
3966 662.8213 2647.2561
3967 884.1804 2649.5194
3968 885.0144 2652.0214
3969 664.0132 2652.0237
3971 664.3331 2653.3033
3972 665.5754 2658.2725
3974 888.7285 2663.1637
3976 892.4935 2674.4587
3977 669.8892 2675.5277
3978 895.1758 2682.5056
3980 895.8347 2684.4823
3981 896.8395 2687.4967
3982 673.0992 2688.3677
3983 897.1349 2688.3829
3984 673.1048 2688.3901
3985 897.1512 2688.4318
3989 902.1439 2703.4099
3991 905.8008 2714.3806
3992 680.1241 2716.4673
3993 908.8497 2723.5273
3994 909.1942 2724.5608
3995 910.1874 2727.5404
3997 683.6052 2730.3917
3999 913.7194 2738.1364
4001 549.4922 2742.4246
4002 686.8640 2743.4269
4003 915.8452 2744.5138
4004 915.8463 2744.5171
4005 687.6659 2746.6345
4007 918.8489 2753.5249
4008 689.8862 2755.5157
4009 690.3743 2757.4681
4010 921.1517 2760.4333
4014 927.7614 2780.2624
4017 928.1380 2781.3922
4018 698.1111 2788.4153
4019 934.8450 2801.5132
4020 935.1891 2802.5455
4021 935.5592 2803.6558
4022 701.9233 2803.6641
4023 703.3460 2809.3549
4024 703.6544 2810.5885
4025 704.3652 2813.4317
4027 939.8502 2816.5288
4028 706.1539 2820.5865
4029 942.1711 2823.4915
4030 943.1348 2826.3826
4031 943.4750 2827.4032
4033 709.1054 2832.3925
4034 945.1422 2832.4048
4037 710.6489 2838.5665
4038 948.8090 2843.4052
4039 949.1550 2844.4432
4040 952.2058 2853.5956
4041 714.8690 2855.4469
4044 716.1749 2860.6705
4045 954.5716 2860.6930
4047 956.8226 2867.4460
4048 959.1861 2874.5365
4049 961.8710 2882.5912
4050 963.5380 2887.5922
4051 578.9067 2889.4971
4052 968.1149 2901.3229
4053 970.4609 2908.3609
4055 972.1337 2913.3793
4056 729.3531 2913.3833
4057 729.3550 2913.3909
4061 584.3065 2916.4961
4062 730.4329 2917.7025
4063 973.9064 2918.6974
4064 975.7773 2924.3101
4065 975.7806 2924.3200
4066 732.3787 2925.4857
4068 979.6630 2935.9672
4069 981.5311 2941.5715
4070 983.1060 2946.2962
4071 991.1418 2970.4036
4072 743.6104 2970.4125
4073 743.6632 2970.6237
4074 992.3099 2973.9079
4076 993.7537 2978.2393
4077 745.5675 2978.2409
4079 746.6458 2982.5541
4080 746.8909 2983.5345
4081 997.1705 2988.4897
4084 755.4300 3017.6909
4085 1007.8050 3020.3932
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