MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3783 599.5520 2394.1789
3784 799.3961 2395.1665
3787 799.4093 2395.2061
3788 800.1052 2397.2938
3795 803.4157 2407.2253
3796 805.0996 2412.2770
3797 805.4225 2413.2457
3798 805.6987 2414.0743
3800 806.7543 2417.2411
3801 808.7678 2423.2816
3802 809.4202 2425.2388
3805 811.4232 2431.2478
3806 811.7437 2432.2093
3807 811.7579 2432.2519
3808 812.4770 2434.4092
3813 814.4254 2440.2544
3814 814.7697 2441.2873
3815 815.4355 2443.2847
3816 816.3438 2446.0096
3817 612.5107 2446.0137
3818 816.4559 2446.3459
3819 816.7569 2447.2489
3821 818.7342 2453.1808
3822 818.7501 2453.2285
3829 616.5714 2462.2565
3831 824.0728 2469.1966
3832 824.0858 2469.2356
3833 618.3168 2469.2381
3835 824.4276 2470.2610
3836 825.0724 2472.1954
3838 496.0583 2475.2551
3839 619.8211 2475.2553
3840 826.1371 2475.3895
3844 829.0603 2484.1591
3846 829.4057 2485.1953
3847 622.3155 2485.2329
3848 831.0615 2490.1627
3853 832.4668 2494.3786
3854 833.0664 2496.1774
3858 627.7930 2507.1429
3859 836.7227 2507.1463
3861 838.3937 2512.1593
3862 838.7170 2513.1292
3865 839.4102 2515.2088
3866 839.4211 2515.2415
3867 840.1087 2517.3043
3868 840.7272 2519.1598
3869 841.0704 2520.1894
3870 631.3051 2521.1913
3871 841.7850 2522.3332
3873 631.7861 2523.1153
3875 842.4659 2524.3759
3878 633.8027 2531.1817
3879 844.7359 2531.1859
3880 844.8043 2531.3911
3881 846.0585 2535.1537
3882 846.0590 2535.1552
3883 848.3978 2542.1716
3884 848.3989 2542.1749
3888 849.1273 2544.3601
3890 849.7706 2546.2900
3892 637.7961 2547.1553
3893 851.0968 2550.2686
3894 851.3824 2551.1254
3895 851.3910 2551.1512
3896 851.8187 2552.4343
3897 853.3873 2557.1401
3898 640.5427 2558.1417
3899 853.7304 2558.1694
3900 854.4011 2560.1815
3901 854.4011 2560.1815
3902 641.0536 2560.1853
3904 854.7326 2561.1760
3906 856.7277 2567.1613
3907 643.0446 2568.1493
3909 858.4039 2572.1899
3910 858.4045 2572.1917
3911 644.2832 2573.1037
3914 644.5353 2574.1121
3915 859.0732 2574.1978
3917 859.4243 2575.2511
3918 644.8235 2575.2649
3919 859.7274 2576.1604
3922 646.5372 2582.1197
3923 861.7184 2582.1334
3925 646.5422 2582.1397
3926 647.3123 2585.2201
3927 864.0307 2589.0703
3929 864.4048 2590.1926
3931 865.1691 2592.4855
3932 865.7623 2594.2651
3933 867.0420 2598.1042
3934 520.6292 2598.1096
3935 650.5351 2598.1113
3936 650.7852 2599.1117
3938 868.0797 2601.2173
3940 870.4432 2608.3078
3942 654.5327 2614.1017
3945 874.3658 2620.0756
3946 874.7231 2621.1475
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