MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 4336)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3405 665.0103 1992.0091
3406 997.0225 1992.0304
3407 666.6708 1996.9906
3408 667.6283 1999.8631
3409 667.9542 2000.8408
3410 1001.4406 2000.8666
3411 669.3704 2005.0894
3412 1004.0510 2006.0874
3415 670.6369 2008.8889
3416 670.6923 2009.0551
3418 1007.5222 2013.0298
3419 1008.5309 2015.0472
3420 672.6901 2015.0485
3424 1011.5055 2020.9964
3425 1011.5831 2021.1516
3427 1012.0762 2022.1378
3429 676.3110 2025.9112
3431 1019.0632 2036.1118
3432 680.2605 2037.7597
3433 682.3527 2044.0363
3435 514.0076 2052.0013
3436 514.0174 2052.0405
3438 685.3542 2053.0408
3439 688.0477 2061.1213
3440 689.0754 2064.2044
3441 1033.5536 2065.0926
3452 518.2722 2069.0597
3453 415.0195 2070.0611
3454 1036.5386 2071.0626
3455 691.3638 2071.0696
3457 1041.4158 2080.8170
3464 1042.7850 2083.5554
3474 697.3835 2089.1287
3475 1045.9364 2089.8582
3476 697.6281 2089.8625
3477 1048.5531 2095.0916
3478 699.7094 2096.1064
3479 1049.9286 2097.8426
3480 700.6231 2098.8475
3481 700.6428 2098.9066
3485 1050.5649 2099.1152
3490 700.9716 2099.8930
3491 700.9720 2099.8942
3494 1051.2917 2100.5688
3495 1052.0417 2102.0688
3497 527.0195 2104.0489
3498 702.7090 2105.1052
3500 1054.0387 2106.0628
3506 1054.9504 2107.8862
3507 703.6365 2107.8877
3509 704.0565 2109.1477
3513 704.9534 2111.8384
3514 1056.9271 2111.8396
3515 1057.0603 2112.1060
3516 705.6157 2113.8253
3517 1058.4529 2114.8912
3519 705.9759 2114.9059
3530 1059.5498 2117.0850
3533 707.0320 2118.0742
3535 531.2227 2120.8617
3537 708.0312 2121.0718
3538 531.2756 2121.0733
3539 708.2989 2121.8749
3540 1062.0330 2122.0514
3541 708.3580 2122.0522
3542 531.5204 2122.0525
3543 708.3621 2122.0645
3544 531.5249 2122.0705
3546 531.7203 2122.8521
3547 708.6285 2122.8637
3548 1062.5272 2123.0398
3549 708.6875 2123.0407
3550 531.7676 2123.0413
3551 1062.9441 2123.8736
3552 708.9670 2123.8792
3553 1064.5023 2126.9900
3554 710.0246 2127.0520
3556 710.0320 2127.0742
3557 1064.5496 2127.0846
3558 710.2766 2127.8080
3559 710.3585 2128.0537
3560 710.6282 2128.8628
3561 710.6429 2128.9069
3562 1065.9607 2129.9068
3563 533.7186 2130.8453
3564 711.2916 2130.8530
3565 711.6455 2131.9147
3566 711.6509 2131.9309
3568 1067.9569 2133.8992
3570 1069.5261 2137.0376
3576 713.6211 2137.8415
3577 535.4680 2137.8429
3578 713.6400 2137.8982
3583 713.6893 2138.0461
3584 535.5222 2138.0597
3585 714.3016 2139.8830
3588 536.2275 2140.8809
3591 1072.5330 2143.0514
3592 1072.8958 2143.7770
3593 715.6001 2143.7785
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