MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3179 521.9530 1562.8372
3180 521.9542 1562.8408
3181 391.7175 1562.8409
3182 782.9238 1563.8330
3186 784.4355 1566.8564
3194 526.5974 1576.7704
3195 395.2032 1576.7837
3200 528.2488 1581.7246
3205 796.3608 1590.7070
3212 533.5660 1597.6762
3214 533.5731 1597.6975
3218 534.9604 1601.8594
3221 402.2028 1604.7821
3223 536.5839 1606.7299
3227 804.9167 1607.8188
3229 805.3990 1608.7834
3231 805.9226 1609.8306
3232 537.6177 1609.8313
3233 537.6200 1609.8382
3234 537.6206 1609.8400
3235 806.4282 1610.8418
3236 538.8908 1613.6506
3238 405.2025 1616.7809
3239 539.9345 1616.7817
3241 540.2641 1617.7705
3243 541.2902 1620.8488
3246 542.5765 1624.7077
3247 544.2192 1629.6358
3252 409.4429 1633.7425
3253 545.6441 1633.9105
3254 409.4851 1633.9113
3257 549.5433 1645.6081
3259 827.4345 1652.8544
3261 552.0043 1652.9911
3263 553.9627 1658.8663
3264 830.4462 1658.8778
3268 416.7375 1662.9209
3271 557.3008 1668.8806
3272 558.2726 1671.7960
3275 837.9275 1673.8404
3276 841.4278 1680.8410
3278 563.5944 1687.7614
3281 848.8534 1695.6922
3282 849.3672 1696.7198
3286 569.2519 1704.7339
3288 427.7372 1706.9197
3290 575.9861 1724.9365
3291 863.8439 1725.6732
3292 576.2424 1725.7054
3293 863.9196 1725.8246
3295 871.8438 1741.6730
3296 581.5657 1741.6753
3299 436.6769 1742.6785
3300 875.9922 1749.9698
3301 877.8809 1753.7472
3302 878.4116 1754.8086
3304 878.9218 1755.8290
3311 440.9886 1759.9253
3319 886.3909 1770.7672
3320 886.7522 1771.4898
3325 597.6093 1789.8061
3326 449.4806 1793.8933
3327 897.9546 1793.8946
3328 599.3058 1794.8956
3329 902.9825 1803.9504
3333 604.0307 1809.0703
3334 906.3044 1810.5942
3336 608.3524 1822.0354
3339 613.6190 1837.8352
3340 920.3833 1838.7520
3341 922.4333 1842.8520
3347 925.5370 1849.0594
3349 464.4933 1853.9441
3354 625.9478 1874.8216
3355 938.4852 1874.9558
3356 630.2827 1887.8263
3357 632.6348 1894.8826
3359 951.9954 1901.9762
3361 476.9876 1903.9213
3362 635.9849 1904.9329
3364 955.5097 1909.0048
3365 637.7281 1910.1625
3370 639.6609 1915.9609
3372 959.4874 1916.9602
3373 961.6935 1921.3724
3375 966.9948 1931.9750
3376 967.4840 1932.9534
3377 969.0444 1936.0742
3378 969.9762 1937.9378
3380 651.6590 1951.9552
3381 977.9584 1953.9022
3382 652.3094 1953.9064
3387 656.7300 1967.1682
3389 493.2531 1968.9833
3393 988.3133 1974.6120
3394 988.5122 1975.0098
3398 992.4233 1982.8320
3399 662.2877 1983.8413
3400 663.3499 1987.0279
3401 664.3564 1990.0474
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