MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2897 658.8500 1315.6854
2898 659.4178 1316.8210
2899 659.9109 1317.8072
2900 661.2462 1320.4778
2903 441.5723 1321.6951
2904 441.5723 1321.6951
2906 441.5730 1321.6972
2907 662.2707 1322.5268
2908 441.8502 1322.5288
2909 662.4056 1322.7966
2913 663.5980 1325.1814
2914 663.5980 1325.1814
2915 663.5986 1325.1826
2916 663.5994 1325.1842
2917 444.8622 1331.5648
2919 333.8986 1331.5653
2924 670.8410 1339.6674
2925 670.9021 1339.7896
2930 452.9249 1355.7529
2934 680.9107 1359.8068
2938 683.1086 1364.2026
2939 458.2490 1371.7252
2940 687.9261 1373.8376
2943 690.8340 1379.6534
2948 465.6062 1393.7968
2950 699.3192 1396.6238
2951 467.2281 1398.6625
2955 469.5878 1405.7416
2959 705.3466 1408.6786
2960 709.8285 1417.6424
2965 475.2493 1422.7261
2989 716.8729 1431.7312
2990 359.7005 1434.7729
2991 479.2655 1434.7747
2992 479.2672 1434.7798
2994 720.3188 1438.6230
3002 481.2429 1440.7069
3006 482.8981 1445.6725
3016 363.1917 1448.7377
3018 726.8599 1451.7052
3025 729.8394 1457.6642
3026 729.8412 1457.6678
3033 488.2243 1461.6511
3044 737.8611 1473.7076
3045 738.4525 1474.8904
3049 739.3674 1476.7202
3052 370.4131 1477.6233
3061 740.4302 1478.8458
3066 741.8769 1481.7392
3069 742.8574 1483.7002
3072 496.5461 1486.6165
3074 373.1994 1488.7685
3075 497.2638 1488.7696
3077 745.8428 1489.6710
3078 497.5651 1489.6735
3079 373.4257 1489.6737
3082 497.9370 1490.7892
3083 498.6061 1492.7965
3084 498.8808 1493.6206
3087 499.8781 1496.6125
3088 749.8746 1497.7346
3089 749.9355 1497.8564
3090 750.8098 1499.6050
3092 501.5347 1501.5823
3093 501.5348 1501.5826
3094 502.5951 1504.7635
3095 502.6026 1504.7860
3096 753.8295 1505.6444
3113 502.9896 1505.9470
3116 504.6063 1510.7971
3117 506.1961 1515.5665
3118 506.1968 1515.5686
3119 758.7922 1515.5698
3120 758.7939 1515.5732
3128 508.2771 1521.8095
3132 508.2794 1521.8164
3133 762.1069 1522.1992
3135 762.9287 1523.8428
3137 509.9315 1526.7727
3139 765.4056 1528.7966
3141 766.3654 1530.7162
3142 511.2654 1530.7744
3143 766.7766 1531.5386
3144 511.5214 1531.5424
3145 511.5215 1531.5427
3148 512.5562 1534.6468
3151 512.6545 1534.9417
3152 513.6083 1537.8031
3153 513.6100 1537.8082
3155 771.9313 1541.8480
3156 772.8862 1543.7578
3157 386.9471 1543.7593
3158 515.5941 1543.7605
3166 518.2771 1551.8095
3172 518.9418 1553.8036
3173 778.9218 1555.8290
3174 520.5820 1558.7242
3176 391.1902 1560.7317
3177 781.3745 1560.7344
3178 391.7163 1562.8361
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