MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2648 541.2487 1080.4828
2649 361.1683 1080.4831
2650 361.8439 1082.5099
2652 361.8814 1082.6224
2655 1085.5564 1084.5491
2661 364.8994 1091.6764
2663 364.8999 1091.6779
2670 1098.5552 1097.5479
2671 367.1670 1098.4792
2672 550.2491 1098.4836
2674 1102.0870 1101.0797
2678 372.5091 1114.5055
2681 1115.5416 1114.5343
2684 559.2534 1116.4922
2689 559.8171 1117.6196
2691 373.9063 1118.6971
2692 374.2388 1119.6946
2694 377.5294 1129.5664
2696 377.8348 1130.4826
2697 1132.5881 1131.5808
2698 378.9007 1133.6803
2699 568.3120 1134.6094
2701 379.2377 1134.6913
2702 568.7811 1135.5476
2704 1136.6003 1135.5930
2705 1136.8778 1135.8705
2706 1139.5826 1138.5753
2707 380.5447 1138.6123
2708 1143.2122 1142.2049
2710 382.1907 1143.5503
2711 572.7880 1143.5614
2712 382.4938 1144.4596
2715 383.1581 1146.4525
2716 1147.4613 1146.4540
2722 577.2932 1152.5718
2723 578.2288 1154.4430
2728 388.5905 1162.7497
2729 583.7295 1165.4444
2730 583.8200 1165.6254
2731 1167.5933 1166.5860
2732 584.8581 1167.7016
2735 586.2135 1170.4124
2738 1171.8060 1170.7987
2739 391.5798 1171.7176
2740 391.8596 1172.5570
2747 593.2075 1184.4004
2751 595.3369 1188.6592
2752 397.5817 1189.7233
2753 397.5821 1189.7245
2763 398.1967 1191.5683
2765 398.8826 1193.6260
2766 598.7902 1195.5658
2770 601.1939 1200.3732
2773 301.6963 1202.7561
2776 603.8514 1205.6882
2778 302.6943 1206.7481
2780 302.6945 1206.7489
2781 302.6951 1206.7513
2782 403.2580 1206.7522
2783 403.2584 1206.7534
2792 406.9273 1217.7601
2793 305.4474 1217.7605
2795 407.2569 1218.7489
2796 407.2578 1218.7516
2797 611.7707 1221.5268
2798 408.5266 1222.5580
2803 409.5936 1225.7590
2812 308.9521 1231.7793
2813 617.7715 1233.5284
2815 618.8580 1235.7014
2818 619.3502 1236.6858
2819 620.7764 1239.5382
2823 622.8218 1243.6290
2831 624.3906 1246.7666
2833 312.9511 1247.7753
2835 624.8950 1247.7754
2836 312.9512 1247.7757
2838 416.9329 1247.7769
2840 416.9331 1247.7775
2849 632.9457 1263.8768
2850 422.5635 1264.6687
2852 423.1634 1266.4684
2853 634.2820 1266.5494
2854 634.2869 1266.5592
2864 426.1564 1275.4474
2872 642.3658 1282.7170
2874 429.5428 1285.6066
2876 646.3026 1290.5906
2877 431.2688 1290.7846
2878 646.7798 1291.5450
2879 431.8635 1292.5687
2880 647.8261 1293.6376
2882 649.8436 1297.6726
2884 434.2120 1299.6142
2886 434.8752 1301.6038
2888 435.9018 1304.6836
2890 436.5260 1306.5562
2891 654.2859 1306.5572
2892 654.8364 1307.6582
2896 658.8038 1315.5930
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