MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2318 353.6948 705.3750
2324 712.8596 711.8523
2325 712.8621 711.8548
2326 712.8647 711.8574
2327 357.1386 712.2626
2333 359.6759 717.3372
2336 367.6627 733.3108
2338 367.6931 733.3716
2341 369.2148 736.4150
2342 739.3613 738.3540
2343 370.7078 739.4010
2349 758.3949 757.3876
2351 380.2365 758.4584
2356 385.1547 768.2948
2357 770.3578 769.3505
2358 773.1182 772.1109
2362 778.4966 777.4893
2363 389.7520 777.4894
2369 783.4513 782.4440
2383 399.6565 797.2984
2394 405.1948 808.3750
2398 409.1825 816.3504
2399 823.1153 822.1080
2403 413.2333 824.4520
2405 833.4390 832.4317
2406 833.4443 832.4370
2419 865.0563 864.0490
2422 436.2479 870.4812
2426 878.4629 877.4556
2427 885.4734 884.4661
2428 886.0410 885.0337
2432 891.1727 890.1654
2435 448.7199 895.4252
2437 300.4842 898.4308
2439 899.4622 898.4549
2443 454.2689 906.5232
2444 454.2848 906.5550
2458 307.8594 920.5564
2459 307.8605 920.5597
2462 462.2150 922.4154
2463 463.7010 925.3874
2466 930.6129 929.6056
2468 466.7353 931.4560
2469 466.7356 931.4566
2470 311.5222 931.5448
2473 312.5151 934.5235
2480 314.1460 939.4162
2484 944.2444 943.2371
2485 472.7359 943.4572
2486 944.4699 943.4626
2489 476.2887 950.5628
2491 476.7801 951.5456
2492 318.8089 953.4049
2494 477.7298 953.4450
2495 955.4852 954.4779
2505 961.4783 960.4710
2507 481.2737 960.5328
2513 484.7379 967.4612
2514 970.3835 969.3762
2515 324.1328 969.3766
2516 485.6958 969.3770
2520 324.5142 970.5208
2524 487.2500 972.4854
2526 325.5350 973.5832
2531 327.2074 978.6004
2532 490.3075 978.6004
2533 490.3089 978.6032
2535 981.3790 980.3717
2543 330.1953 987.5641
2544 495.2518 988.4890
2558 332.1346 993.3820
2563 500.7079 999.4012
2565 502.7652 1003.5158
2566 503.7958 1005.5770
2567 336.8706 1007.5900
2571 505.2278 1008.4410
2573 338.1895 1011.5467
2574 506.7812 1011.5478
2579 340.2106 1017.6100
2582 512.6556 1023.2966
2586 1025.5096 1024.5023
2591 514.6894 1027.3642
2601 348.2032 1041.5878
2602 348.8466 1043.5180
2603 1044.9327 1043.9254
2604 523.2617 1044.5088
2609 1048.5303 1047.5230
2614 350.5474 1048.6204
2615 525.3179 1048.6212
2616 351.1790 1050.5152
2619 1052.5269 1051.5196
2620 528.7979 1055.5812
2626 355.8442 1064.5108
2627 533.2631 1064.5116
2628 1065.5448 1064.5375
2632 535.2927 1068.5708
2636 357.8512 1070.5318
2639 536.7892 1071.5638
2646 539.8433 1077.6720
2647 1080.5486 1079.5413
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