MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2149 535.2538 534.2465
2150 536.2585 535.2512
2151 537.2335 536.2262
2152 539.1582 538.1509
2154 539.3951 538.3878
2155 540.9000 539.8927
2156 542.2827 541.2754
2157 543.1540 542.1467
2158 543.1548 542.1475
2159 545.1353 544.1280
2160 547.1500 546.1427
2161 547.1513 546.1440
2162 548.0714 547.0641
2163 548.2847 547.2774
2164 550.2662 549.2589
2167 553.1396 552.1323
2168 555.0276 554.0203
2169 559.2693 558.2620
2170 559.3255 558.3182
2171 561.1666 560.1593
2172 561.1790 560.1717
2173 562.0873 561.0800
2174 563.1823 562.1750
2176 563.2845 562.2772
2177 564.3615 563.3542
2178 566.3055 565.2982
2179 567.2438 566.2365
2180 567.2446 566.2373
2181 569.2198 568.2125
2182 569.3166 568.3093
2184 573.2777 572.2704
2187 581.2578 580.2505
2189 584.2646 583.2573
2190 585.0984 584.0911
2191 585.1651 584.1578
2192 589.1740 588.1667
2193 589.1843 588.1770
2195 592.3456 591.3383
2196 594.3752 593.3679
2197 595.2727 594.2654
2198 595.2728 594.2655
2199 599.1399 598.1326
2200 599.1807 598.1734
2201 599.3433 598.3360
2203 601.0699 600.0626
2204 301.1488 600.2830
2205 301.6402 601.2658
2206 302.1132 602.2118
2208 605.2145 604.2072
2209 303.1112 604.2078
2212 303.2058 604.3970
2213 303.2142 604.4138
2217 611.2446 610.2373
2218 307.1024 612.1902
2219 307.1024 612.1902
2220 307.1142 612.2138
2221 307.1484 612.2822
2222 307.1576 612.3006
2223 614.2757 613.2684
2224 308.1565 614.2984
2225 308.1567 614.2988
2226 308.6481 615.2816
2228 616.2913 615.2840
2231 619.1351 618.1278
2234 621.0729 620.0656
2237 623.2318 622.2245
2243 630.0013 628.9940
2244 316.1096 630.2046
2253 320.6278 639.2410
2254 320.6322 639.2498
2255 640.2578 639.2505
2256 321.1919 640.3692
2257 641.3768 640.3695
2261 643.0548 642.0475
2262 643.1193 642.1120
2263 324.0185 646.0224
2264 325.1715 648.3284
2265 650.0268 649.0195
2267 651.0825 650.0752
2268 326.1557 650.2968
2271 327.6224 653.2302
2272 656.2526 655.2453
2273 329.1270 656.2394
2274 329.9788 657.9430
2275 330.0479 658.0812
2277 659.3500 658.3427
2279 330.6306 659.2466
2280 331.0901 660.1656
2282 333.1477 664.2808
2283 665.3643 664.3570
2289 334.6100 667.2054
2290 335.6085 669.2024
2292 336.7070 671.3994
2294 337.1129 672.2112
2305 341.1764 680.3382
2311 699.3221 698.3148
2312 700.8385 699.8312
2313 351.1420 700.2694
2314 352.6817 703.3488
2315 705.3456 704.3383
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