MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4336)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2038 469.1187 468.1114
2039 469.1192 468.1119
2040 469.2415 468.2342
2041 469.2430 468.2357
2042 469.2442 468.2369
2043 470.4228 469.4155
2044 471.0979 470.0906
2045 471.1143 470.1070
2046 471.1335 470.1262
2047 471.1337 470.1264
2048 472.2907 471.2834
2049 473.0773 472.0700
2050 473.1139 472.1066
2051 473.1140 472.1067
2052 473.1485 472.1412
2053 473.1627 472.1554
2054 474.9814 473.9741
2055 475.0934 474.0861
2056 475.1263 474.1190
2057 475.3263 474.3190
2059 476.2833 475.2760
2060 478.0653 477.0580
2061 478.2310 477.2237
2062 479.2292 478.2219
2063 479.2307 478.2234
2064 479.2622 478.2549
2065 481.1184 480.1111
2066 481.2625 480.2552
2067 483.0646 482.0573
2068 483.1344 482.1271
2069 483.1817 482.1744
2070 483.2457 482.2384
2071 484.0688 483.0615
2072 485.1142 484.1069
2073 485.1282 484.1209
2074 485.1497 484.1424
2075 487.0876 486.0803
2076 487.1267 486.1194
2077 487.1295 486.1222
2078 487.1296 486.1223
2080 488.2279 487.2206
2081 489.0822 488.0749
2082 489.1089 488.1016
2083 489.1094 488.1021
2085 492.2278 491.2205
2086 493.0216 492.0143
2087 493.0219 492.0146
2088 493.0224 492.0151
2089 493.0230 492.0157
2090 493.1941 492.1868
2091 493.2470 492.2397
2092 495.2225 494.2152
2094 495.2618 494.2545
2095 495.2619 494.2546
2096 496.2797 495.2724
2097 497.1703 496.1630
2098 497.2726 496.2653
2099 497.2761 496.2688
2100 499.0374 498.0301
2101 499.2908 498.2835
2102 500.2750 499.2677
2103 501.1093 500.1020
2104 501.1451 500.1378
2105 501.1451 500.1378
2106 501.1454 500.1381
2107 502.3384 501.3311
2108 503.1258 502.1185
2109 504.2256 503.2183
2110 505.1415 504.1342
2111 507.2220 506.2147
2112 508.2887 507.2814
2113 509.1978 508.1905
2114 510.2402 509.2329
2115 511.1301 510.1228
2116 512.3088 511.3015
2117 513.1426 512.1353
2118 513.1442 512.1369
2119 515.1246 514.1173
2120 515.1248 514.1175
2123 517.1404 516.1331
2124 517.1887 516.1814
2125 517.3127 516.3054
2126 518.0610 517.0537
2127 519.1193 518.1120
2128 519.1567 518.1494
2129 520.0768 519.0695
2130 520.2310 519.2237
2131 520.2322 519.2249
2132 522.2363 521.2290
2133 525.2816 524.2743
2134 525.2898 524.2825
2135 526.2363 525.2290
2136 527.1606 526.1533
2138 528.3058 527.2985
2139 529.3354 528.3281
2141 532.0759 531.0686
2142 533.1361 532.1288
2143 533.1362 532.1289
2147 533.3317 532.3244
2148 535.0930 534.0857
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