MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy2682a
MS data file : Andy2682a.mgf
Database : P-putida 20180924 (5,556 sequences; 1,892,173 residues)
Timestamp : 2 Aug 2026 at 00:52:38 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Oxidation (M), Deamidated (NQ)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 50 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : Default
Number of queries : 4,656

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 20 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 4336)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2530 327.1672 978.4798 978.5056 -26.3 1 8 0.42 +1Score > 22 indicates identity
Score > 17 indicates homology
MVKQIESK + Oxidation (M); Deamidated (NQ)
2830 623.8493 1245.6840 1245.7121 -22.5 0 8 0.77 +1Score > 22 indicates identity
Score > 20 indicates homology
ILEFLAVGAWK
2651 542.3137 1082.6128 1082.6560 -39.9 1 8 0.52 +1Score > 20 indicates identity
Score > 18 indicates homology
GVRLADIALR
2669 549.7789 1097.5432 1097.5427 0.53 1 8 0.66 +1Score > 21 indicates identity
Score > 19 indicates homology
YMLKVNDAK + Oxidation (M); Deamidated (NQ)
3011 483.5616 1447.6630 1447.6976 -24.0 1 8 0.75 +1Score > 21 indicates identity
Score > 20 indicates homology
EELVQAAEKQMR + Oxidation (M); Deamidated (NQ)
3081 373.7039 1490.7865 1490.8569 -47.2 0 8 0.51 +1Score > 23 indicates identity
Score > 18 indicates homology
LVVVGVAGGNLHSIR + Deamidated (NQ)
3207 531.2604 1590.7594 1590.7372 13.9 0 8 0.29 +1Score > 23 indicates identity
Score > 15 indicates homology
SSAELAQETANAAAQK + 2 Deamidated (NQ)
2713 1146.7203 1145.7130 1145.7060 6.16 0 8 0.15 +1Score > 13 indicates identity QSLILAILFK + Deamidated (NQ)
2829 623.8182 1245.6218 1245.6023 15.7 0 8 0.69 +1Score > 23 indicates identity
Score > 19 indicates homology
IVEDMQDGLAR
2079 487.3359 486.3286 486.3166 24.8 0 8 1 +1Score > 22 indicates identity
Score > 21 indicates homology
VGLAK
3032 731.8321 1461.6496 1461.6848 -24.0 1 8 0.66 +1Score > 20 indicates identity
Score > 19 indicates homology
EYDAQPRDLQAR + Deamidated (NQ)
2421 435.7758 869.5370 869.5123 28.4 0 8 0.65 +1Score > 19 indicates identity VTVPWLR
3211 797.9275 1593.8404 1593.8185 13.8 0 8 0.28 +1Score > 21 indicates identity
Score > 15 indicates homology
TTFAMNLVENAVLR + Oxidation (M)
3505 527.7741 2107.0673 2106.9759 43.4 0 8 0.96 +1Score > 23 indicates identity
Score > 20 indicates homology
GNQPQPYQVDGQTWYGIR + Deamidated (NQ)
2785 303.3965 1209.5569 1209.5560 0.70 0 8 0.75 +1Score > 21 indicates identity
Score > 19 indicates homology
MAAGSNQTFQR
3575 713.3634 2137.0684 2136.9716 45.3 0 8 0.91 +1Score > 23 indicates identity
Score > 20 indicates homology
DLQMPPPGMLLFGGMMVNR + 2 Oxidation (M); 2 Deamidated (NQ)
2354 767.3519 766.3446 766.3643 -25.7 1 8 0.75 +1Score > 19 indicates identity MKTTDR + Oxidation (M)
2638 358.1949 1071.5629 1071.5171 42.7 1 8 1 +1Score > 23 indicates identity
Score > 20 indicates homology
CFKAYDIR
2528 489.2591 976.5036 976.5342 -31.3 0 8 0.9 +1Score > 23 indicates identity
Score > 20 indicates homology
FAGQQLSVK
3316 588.6285 1762.8637 1762.9141 -28.6 0 8 0.26 +1Score > 23 indicates identity
Score > 14 indicates homology
SEPDQDLLLFLFGLR + Deamidated (NQ)
3206 398.6971 1590.7593 1590.7372 13.9 0 8 1 +1Score > 23 indicates identity
Score > 20 indicates homology
SSAELAQETANAAAQK + 2 Deamidated (NQ)
2359 387.6893 773.3640 773.3668 -3.51 0 8 0.24 +1Score > 21 indicates identity
Score > 14 indicates homology
IDGQNAR + Deamidated (NQ)
2547 331.2091 990.6055 990.5723 33.5 1 8 0.2 +1Score > 13 indicates identity RLPALHQR + Deamidated (NQ)
2996 720.8574 1439.7002 1439.6523 33.3 0 8 0.33 +1Score > 21 indicates identity
Score > 15 indicates homology
LSLEEMMVQSEK + Oxidation (M); Deamidated (NQ)
2560 498.7827 995.5508 995.5110 40.0 0 8 0.78 +1Score > 19 indicates identity VLMQGAFSK + Oxidation (M)
2645 360.2308 1077.6706 1077.6182 48.6 1 8 0.26 +1Score > 14 indicates identity LFTQTAIKR + Deamidated (NQ)
3003 722.3531 1442.6916 1442.6898 1.31 1 8 0.67 +1Score > 22 indicates identity
Score > 18 indicates homology
VMKNDISQMFAK + 2 Oxidation (M)
2862 424.2188 1269.6346 1269.6830 -38.1 1 8 0.82 +1Score > 22 indicates identity
Score > 19 indicates homology
LESWRQGPLGK
2411 421.2323 840.4500 840.4341 18.9 0 8 0.85 +1Score > 19 indicates identity ATVAHELT
3039 733.3438 1464.6730 1464.7177 -30.5 1 7 0.77 +1Score > 22 indicates identity
Score > 19 indicates homology
MVSLMTQDARAAR + Oxidation (M)
3395 660.3352 1977.9838 1977.9578 13.1 1 7 0.37 +1Score > 23 indicates identity
Score > 16 indicates homology
AYGAVRMGLIDNVDQAAGR + 2 Deamidated (NQ)
2570 504.8038 1007.5930 1007.5624 30.4 1 7 0.49 +1Score > 17 indicates identity ALRDHGIAR
2618 526.3320 1050.6494 1050.6590 -9.06 1 7 0.18 +1Score > 13 indicates identity LVYKLFIR
3064 741.3700 1480.7254 1480.7232 1.55 0 7 0.74 +1Score > 22 indicates identity
Score > 19 indicates homology
VADMVIQAEYVAR + Oxidation (M); Deamidated (NQ)
2364 779.4057 778.3984 778.4337 -45.3 1 7 0.44 +1Score > 22 indicates identity
Score > 16 indicates homology
LREYAK
2881 647.8303 1293.6460 1293.6564 -8.04 1 7 0.22 +1Score > 23 indicates identity
Score > 13 indicates homology
DPVKALQQHEK + 2 Deamidated (NQ)
2360 776.3870 775.3797 775.3898 -13.0 0 7 0.51 +1Score > 21 indicates identity
Score > 17 indicates homology
MQLINR + 2 Deamidated (NQ)
2606 523.7635 1045.5124 1045.5161 -3.48 1 7 0.66 +1Score > 23 indicates identity
Score > 18 indicates homology
MSKIMHAGR + Oxidation (M)
2824 623.3082 1244.6018 1244.5884 10.8 1 7 0.73 +1Score > 23 indicates identity
Score > 18 indicates homology
LKGSPEDNEQK + Deamidated (NQ)
3582 535.5181 2138.0433 2138.1154 -33.7 1 7 0.31 +1Score > 22 indicates identity
Score > 15 indicates homology
QSAVLDCHKGITLALANAVR + 2 Deamidated (NQ)
2521 486.2684 970.5222 970.5447 -23.2 1 7 0.25 +1Score > 22 indicates identity
Score > 14 indicates homology
QQTPKIQK + Deamidated (NQ)
2787 404.5465 1210.6177 1210.6095 6.78 1 7 0.95 +1Score > 22 indicates identity
Score > 20 indicates homology
RFYDQGGLAGK
2522 486.2684 970.5222 970.5447 -23.2 0 7 0.75 +1Score > 22 indicates identity
Score > 18 indicates homology
NGLLSPVGSK
2569 504.8038 1007.5930 1007.5624 30.4 1 7 0.53 +1Score > 17 indicates identity ALRDHGIAR
2375 393.2310 784.4474 784.4807 -42.3 0 7 0.81 +1Score > 20 indicates identity
Score > 19 indicates homology
EALIALR
2807 410.5735 1228.6987 1228.6815 13.9 0 7 0.35 +1Score > 21 indicates identity
Score > 15 indicates homology
LVQFLQLNPR + 2 Deamidated (NQ)
2559 332.8574 995.5504 995.5764 -26.1 0 7 0.89 +1Score > 19 indicates identity IGLLGPNGAGK
3258 825.3630 1648.7114 1648.7072 2.56 0 7 0.87 +1Score > 19 indicates identity MSNPNEDSPMILQR + Oxidation (M); 2 Deamidated (NQ)
3771 794.0867 2379.2383 2379.1554 34.8 0 7 1 +1Score > 22 indicates identity
Score > 20 indicates homology
QLYGGQVLGQSLSAASQTVEDAR + 2 Deamidated (NQ)
2508 481.3021 960.5896 960.5426 49.0 1 7 0.35 +1Score > 15 indicates identity MTELARLK
3324 594.6446 1780.9120 1780.8665 25.5 0 7 0.3 +1Score > 23 indicates identity
Score > 14 indicates homology
AAAADIQAHLEQVAMPK + Oxidation (M); 2 Deamidated (NQ)
2365 390.2435 778.4724 778.4337 49.8 1 7 0.7 +1Score > 18 indicates identity LAREYK
2384 801.4082 800.4009 800.4028 -2.35 0 7 0.49 +1Score > 21 indicates identity
Score > 16 indicates homology
LQPEASR + Deamidated (NQ)
2588 1027.5714 1026.5641 1026.5280 35.1 0 7 0.56 +1Score > 21 indicates identity
Score > 17 indicates homology
HCTLVLQR + Deamidated (NQ)
2743 590.2957 1178.5768 1178.5819 -4.28 0 7 0.33 +1Score > 22 indicates identity
Score > 15 indicates homology
IVEANVDYEK
2841 313.1972 1248.7597 1248.7302 23.6 1 7 0.26 +1Score > 14 indicates identity DHLLLERVVR
3188 784.4467 1566.8788 1566.8504 18.1 0 7 0.38 +1Score > 20 indicates identity
Score > 15 indicates homology
QLAQELDLVLEAPK + Deamidated (NQ)
2595 515.7779 1029.5412 1029.5567 -15.0 1 7 0.37 +1Score > 23 indicates identity
Score > 15 indicates homology
RIITDAEGR
2962 710.8606 1419.7066 1419.7544 -33.6 1 7 0.26 +1Score > 23 indicates identity
Score > 14 indicates homology
LLNLFNEMRAAK + Deamidated (NQ)
3740 782.0679 2343.1819 2343.1165 27.9 1 7 0.34 +1Score > 23 indicates identity
Score > 15 indicates homology
MLDDLLEGRDGQLLANPHYR + Oxidation (M); 2 Deamidated (NQ)
2575 338.1900 1011.5482 1011.5964 -47.7 1 7 0.87 +1Score > 20 indicates identity
Score > 19 indicates homology
NIPLKLQGK + 2 Deamidated (NQ)
3345 923.9700 1845.9254 1845.8679 31.2 0 7 0.29 +1Score > 23 indicates identity
Score > 14 indicates homology
LFQHTLECGNITAGAGR + 2 Deamidated (NQ)
3658 737.0723 2208.1951 2208.2154 -9.21 0 7 0.23 +1Score > 20 indicates identity
Score > 13 indicates homology
QVQVVFQLQPEAQLTPLLR + 2 Deamidated (NQ)
2058 476.2272 475.2199 475.2390 -40.2 0 7 0.54 +1Score > 24 indicates identity
Score > 17 indicates homology
NAGSK
2794 610.3480 1218.6814 1218.6278 44.0 0 7 1 +1Score > 21 indicates identity
Score > 19 indicates homology
LAMLDAATELR + Oxidation (M)
3175 780.8845 1559.7544 1559.7614 -4.43 0 7 1 +1Score > 23 indicates identity
Score > 19 indicates homology
AMDQGVVVINIDNR + Oxidation (M); Deamidated (NQ)
2368 781.4485 780.4412 780.4395 2.23 0 7 0.71 +1Score > 18 indicates identity LWAVHR
2845 632.3617 1262.7088 1262.6652 34.5 1 7 0.49 +1Score > 20 indicates identity
Score > 16 indicates homology
TQQRLLSLMR + Oxidation (M); 2 Deamidated (NQ)
2944 693.3516 1384.6886 1384.6946 -4.33 0 7 0.41 +1Score > 22 indicates identity
Score > 15 indicates homology
LSDDLQNLNPTR
3297 871.9619 1741.9092 1741.9210 -6.72 1 7 1 +1Score > 22 indicates identity
Score > 19 indicates homology
QALQEAEISLANAKQK + Deamidated (NQ)
3073 496.6014 1486.7824 1486.8103 -18.8 1 7 0.71 +1Score > 23 indicates identity
Score > 18 indicates homology
LAGLTATEQRAVTR + Deamidated (NQ)
3159 772.8954 1543.7762 1543.7842 -5.15 1 7 0.46 +1Score > 23 indicates identity
Score > 16 indicates homology
KTLDTQTGQPVEAR + Deamidated (NQ)
2175 563.2627 562.2554 562.2347 36.9 0 7 0.58 +1Score > 20 indicates identity
Score > 17 indicates homology
NSDAR + Deamidated (NQ)
2737 391.2145 1170.6217 1170.5921 25.3 0 7 0.32 +1Score > 23 indicates identity
Score > 14 indicates homology
QQAYLATTFK + Deamidated (NQ)
3053 739.8190 1477.6234 1477.6547 -21.2 0 7 0.79 +1Score > 18 indicates identity AIEQFMQQYYR + 2 Deamidated (NQ)
2688 559.8070 1117.5994 1117.5563 38.6 1 7 0.27 +1Score > 23 indicates identity
Score > 13 indicates homology
HDIPMGRHR
3390 657.3741 1969.1005 1969.0619 19.6 0 6 0.97 +1Score > 19 indicates identity LDLLTAEVVESLNAILQQ + Deamidated (NQ)
2607 349.5116 1045.5130 1045.5161 -2.98 1 6 0.49 +1Score > 23 indicates identity
Score > 16 indicates homology
MSKIMHAGR + Oxidation (M)
3803 810.0646 2427.1720 2427.1603 4.82 0 6 0.26 +1Score > 23 indicates identity
Score > 13 indicates homology
FIGVGGPLMEAEGLQSYFPMER
4096 769.6843 3074.7081 3074.6012 34.8 1 6 0.75 +1Score > 18 indicates identity SVDQPWVRIPFPGAPEQGMLVIELPQR + Oxidation (M)
2775 603.8010 1205.5874 1205.5677 16.4 0 6 0.42 +1Score > 22 indicates identity
Score > 15 indicates homology
LAGTDPDYAQR
2418 432.2532 862.4918 862.4582 39.0 0 6 0.44 +1Score > 20 indicates identity
Score > 15 indicates homology
QVAMALSK + Oxidation (M)
3343 462.2302 1844.8917 1844.8363 30.0 1 6 0.32 +1Score > 23 indicates identity
Score > 14 indicates homology
QAQVFDSKAAQQYMGR + Oxidation (M); 2 Deamidated (NQ)
2431 445.2635 888.5124 888.5029 10.8 1 6 0.85 +1Score > 20 indicates identity
Score > 18 indicates homology
KDLLSSAR
2871 640.8296 1279.6446 1279.6408 2.99 0 6 0.87 +1Score > 22 indicates identity
Score > 18 indicates homology
ETGVDYSILAGR
2188 581.3315 580.3242 580.3445 -35.0 0 6 0.83 +1Score > 18 indicates identity VGIHR
2540 492.2650 982.5154 982.5600 -45.3 1 6 0.9 +1Score > 21 indicates identity
Score > 18 indicates homology
KFVIDPHK
2964 475.2400 1422.6982 1422.6959 1.59 1 6 0.3 +1Score > 22 indicates identity
Score > 14 indicates homology
GTTCKQIIQMAR + Oxidation (M); Deamidated (NQ)
2630 533.7539 1065.4932 1065.5342 -38.5 0 6 0.66 +1Score > 21 indicates identity
Score > 17 indicates homology
ATYPSATVEK
3146 512.2780 1533.8122 1533.8263 -9.22 0 6 0.53 +1Score > 23 indicates identity
Score > 16 indicates homology
IAEQAGIQALAVHGR + Deamidated (NQ)
2911 662.8998 1323.7850 1323.8238 -29.3 1 6 0.55 +1Score > 16 indicates identity LEIARALGAVALK
3828 821.7580 2462.2522 2462.2111 16.7 1 6 1 +1Score > 22 indicates identity
Score > 19 indicates homology
LPSIGGVNLSQMGNAQGAAEKQFK + Oxidation (M); 2 Deamidated (NQ)
2887 652.3986 1302.7826 1302.7408 32.1 1 6 0.51 +1Score > 16 indicates identity LAARNLFTELR
4465 1185.6240 4738.4669 4738.3775 18.9 0 6 0.83 +1Score > 18 indicates identity LQGQVFDLIFVNAGVMGPLPQDLEAVHNSDIGDLFMTNAVAPIR + Oxidation (M); Deamidated (NQ)
2493 477.7101 953.4056 953.4427 -38.9 1 6 0.67 +1Score > 17 indicates identity QHNDQRR + Deamidated (NQ)
2285 334.1528 666.2910 666.2829 12.2 0 6 0.67 +1Score > 17 indicates identity MTPMR + 2 Oxidation (M)
2534 981.3771 980.3698 980.4134 -44.5 0 6 0.24 +1Score > 13 indicates identity NSGSAGWMR + Oxidation (M)
3826 821.7491 2462.2255 2462.2032 9.03 0 6 1 +1Score > 22 indicates identity
Score > 19 indicates homology
MINAQLLQSMVDASNDGIVVAEK + Oxidation (M); Deamidated (NQ)
2918 666.7899 1331.5652 1331.6292 -48.0 1 6 0.79 +1Score > 18 indicates identity VFAGPANDPKCR + Deamidated (NQ)
3758 790.4091 2368.2055 2368.1349 29.8 1 6 0.35 +1Score > 23 indicates identity
Score > 14 indicates homology
HYPQGAHFAHSVGYVGRINEK + 2 Deamidated (NQ)
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